Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,558,242 A→G 5.2% K181E (AAG→GAG)  PP_3142 → sugar transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,558,2420AG 5.2% 224.3 / 2.4 76K181E (AAG→GAG) PP_3142sugar transferase
Reads supporting (aligned to +/- strand):  ref base A (34/38);  new base G (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

TGAACTGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCA  >  NC_002947/3558097‑3558386
                                                                                                                                                   |                                                                                                                                                
tgAACTGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAgg                                                                                                                                               >  5:322047/1‑149 (MQ=255)
  aaCTGCAAAAACGCTTTACCGTCCCCGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGc                                                                                                                                             <  4:81938/149‑1 (MQ=255)
  aaCTGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAggg                                                                                                                                              <  3:188022/148‑1 (MQ=255)
  aaCTGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGc                                                                                                                                             <  3:62246/149‑1 (MQ=255)
   aCTGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGc                                                                                                                                             >  2:411822/1‑148 (MQ=255)
     tGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGAt                                                                                                                                          >  8:210719/1‑149 (MQ=255)
       cAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGAt                                                                                                                                          >  7:117179/1‑147 (MQ=255)
       cAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGa                                                                                                                                        <  4:334943/149‑1 (MQ=255)
       cAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGGTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGAt                                                                                                                                          >  3:63497/1‑147 (MQ=255)
        aaaaaCGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAg                                                                                                                                       >  2:179632/1‑149 (MQ=255)
         aaaaCGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTGGACAAGGGCGATGAGc                                                                                                                                      <  1:411822/149‑1 (MQ=255)
            aCGCCTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAg                                                                                                                                   <  1:25726/149‑1 (MQ=255)
              gCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCAATCAGAAAATCAGCAACAAACCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGAAAggg                                                                                                                                 >  7:207501/1‑149 (MQ=255)
               cTTTACCGCCCACGCGAGTCTGGATAATGCCCTTATCATGGGCAAGTGTGACGTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACGCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGc                                                                                                                                <  8:103753/149‑1 (MQ=255)
                 ttACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCg                                                                                                                              >  7:59627/1‑149 (MQ=255)
                   aCCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCgg                                                                                                                             >  1:333881/1‑148 (MQ=255)
                     cGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGGGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGaaa                                                                                                                          <  7:396204/149‑1 (MQ=255)
                        ccACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTg                                                                                                                       >  4:380433/1‑149 (MQ=255)
                                gTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGc                                                                                                               <  6:322047/149‑1 (MQ=255)
                                    ggATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAag                                                                                                            >  2:279462/1‑148 (MQ=255)
                                     gATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAagc                                                                                                           <  7:389525/148‑1 (MQ=255)
                                     gATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAag                                                                                                            >  7:58356/1‑147 (MQ=255)
                                         aTGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAgcg                                                                                                       <  2:190466/148‑1 (MQ=255)
                                             ccTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGc                                                                                                  <  3:380433/149‑1 (MQ=255)
                                             ccTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGc                                                                                                  >  4:310909/1‑149 (MQ=255)
                                                   catcGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGc                                                                                            >  2:271063/1‑149 (MQ=255)
                                                   catcGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGc                                                                                            >  7:231562/1‑149 (MQ=255)
                                                    atcGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGaa                                                                                                                  <  7:101893/126‑1 (MQ=255)
                                                    atcGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGaa                                                                                                                  >  8:101893/1‑126 (MQ=255)
                                                     tcGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTg                                                                                          >  5:286861/1‑149 (MQ=255)
                                                       ggCAAGGGTGACCTTGCTAACTTCCTCTATCAGAAAATCAGCAACAACCCGTTGTTTGGCGAGCGGGTTCTGGTGTGCGT‑G‑TTGCTTGACAAGGGCGATGAGCATGGGCGGGAAAGTGCAGAAGGCAAGCAGCGCCTGCCGGTGCTgg                                                                                         <  1:304008/148‑1 (MQ=255)
                                                       ggCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGACAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTggg                                                                                        <  1:286533/149‑1 (MQ=255)
                                                            cGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGt                                                                                                                        >  7:41118/1‑112 (MQ=255)
                                                            cGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGt                                                                                                                        <  8:41118/112‑1 (MQ=255)
                                                             ggTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCatat                                                                                  >  7:159664/1‑149 (MQ=255)
                                                             ggTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCatat                                                                                  <  3:310909/149‑1 (MQ=255)
                                                              gTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATAtt                                                                                 >  1:75826/1‑149 (MQ=255)
                                                              gTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATAtt                                                                                 <  8:58356/149‑1 (MQ=255)
                                                                 aCCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGc                                                                              <  1:279462/149‑1 (MQ=255)
                                                                   cTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCt                                                                                                    <  1:157920/125‑1 (MQ=255)
                                                                   cTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCt                                                                                                    >  2:157920/1‑125 (MQ=255)
                                                                   cTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTg                                                                                <  1:266503/145‑1 (MQ=255)
                                                                   cTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTg                                                                                >  2:266503/1‑145 (MQ=255)
                                                                      gCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCt                                                                         >  4:179071/1‑149 (MQ=255)
                                                                         aaCTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGTAGAGTGATTGACAAGGGAGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTc                                                                        >  1:131042/1‑149 (MQ=255)
                                                                           cTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACGAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTcgac                                                                     >  4:153951/1‑148 (MQ=255)
                                                                           cTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACGAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTcgac                                                                     >  4:147627/1‑148 (MQ=255)
                                                                                  tATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGTGACCTCGACGAGCtg                                                               <  5:211860/147‑1 (MQ=255)
                                                                                        aaaaTCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCg                                                       >  3:178053/1‑149 (MQ=255)
                                                                                           aTCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAag                                                                                                            <  2:296750/93‑1 (MQ=255)
                                                                                           aTCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAag                                                                                                            >  1:296750/1‑93 (MQ=255)
                                                                                           aTCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggca                                                    >  2:365705/1‑149 (MQ=255)
                                                                                            tCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcat                                                   >  2:89201/1‑149 (MQ=255)
                                                                                              aGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatgg                                                 <  3:185283/149‑1 (MQ=255)
                                                                                              aGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatg                                                  <  1:89201/148‑1 (MQ=255)
                                                                                               gCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCAAATTGGCGACCTCGACGAGCTGGTGGCCCggcatggc                                                <  1:271063/149‑1 (MQ=255)
                                                                                                 aacaacCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACGAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatggcat                                              <  3:153951/149‑1 (MQ=255)
                                                                                                 aacaacCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACGAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatggcat                                              <  3:147627/149‑1 (MQ=255)
                                                                                                 aacaacCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatggcat                                              >  4:6554/1‑149 (MQ=255)
                                                                                                   caacCCGTGGTTTGGCTAGAGGGAGCAGGGCTGCTT‑G‑GTCATTGACAACGGCGATGAGAAAGGGCCGTAAAGTGACGAAGGCAAGAAGCGAATGCCCGTGCTGGGGCTTATTGGCGACCTCGACGAGCTCGTGGCCCGGCATGGCATTc                                            >  6:267289/1‑149 (MQ=255)
                                                                                                      cccGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGATCTGGTGGCCCGGCATGGCATTCGTa                                         >  1:316989/1‑149 (MQ=255)
                                                                                                       ccGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTAc                                        <  1:179632/149‑1 (MQ=255)
                                                                                                          tGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGgt                                     <  8:231562/149‑1 (MQ=255)
                                                                                                            gTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTc                                            >  7:24757/1‑140 (MQ=255)
                                                                                                            gTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTc                                            <  8:24757/140‑1 (MQ=255)
                                                                                                               tGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTAc                                 <  6:286861/148‑1 (MQ=255)
                                                                                                               tGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTAc                                 <  8:117179/148‑1 (MQ=255)
                                                                                                                 gCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTg                              <  3:6554/149‑1 (MQ=255)
                                                                                                                 gCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTg                              >  6:159582/1‑149 (MQ=255)
                                                                                                                  cGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTgg                             <  2:333881/149‑1 (MQ=255)
                                                                                                                   gAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTGGt                            <  8:159664/149‑1 (MQ=255)
                                                                                                                               gggTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGTGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTg                  <  7:330138/147‑1 (MQ=255)
                                                                                                                                ggTGCGT‑G‑GTGCTGGACAAGGGCGATGAGCAAGGGCCGGAACGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGGGGCCCGGCATGGCATTCCTACGGTGTACCTGGTGACGCCGCTgggg               <  4:63497/149‑1 (MQ=255)
                                                                                                                                  tGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTgggggg             <  3:179071/149‑1 (MQ=255)
                                                                                                                                        g‑gtgCTTGACAAGGTGGATGAGCAAGGGCCGGAAAGTGCCGAAGGGAAGCAGCGGCTGCCGGTGCTGGGGCTTAGTGGGGACCTCGACGAGCTGGTGGCGCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCg        <  5:267289/149‑1 (MQ=255)
                                                                                                                                               tGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGtca  >  2:148596/1‑149 (MQ=255)
                                                                                                                                                   aaGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatg                                                  <  4:180215/97‑1 (MQ=255)
                                                                                                                                                   aaGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCggcatg                                                  >  3:180215/1‑97 (MQ=255)
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TGAACTGCAAAAACGCTTTACCGCCCACGCGCGTCTGGATAATGCCCTTATCATCGGCAACGGTGACCTGGCCAACTTCCTCTATCAGAAAATCAGCAACAACCCGTGGTTTGGCGAGCGGGTGCTGGGGTGCGT‑G‑GTGCTTGACAAGGGCGATGAGCAAGGGCCGGAAAGTGCCGAAGGCAAGCAGCGCCTGCCGGTGCTGGGGCATATTGGCGACCTCGACGAGCTGGTGGCCCGGCATGGCATTCGTACGGTGTACCTGGTGACGCCGCTGGGGGGCTCCGAAGTCA  >  NC_002947/3558097‑3558386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑