| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,578,429 | T→A | 7.1% | intergenic (+106/‑218) | PP_3158 → / → PP_5589 | hypothetical protein/rhs repeat‑associated core domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,578,429 | 0 | T | A | 7.1% | 166.3 / 3.1 | 56 | intergenic (+106/‑218) | PP_3158/PP_5589 | hypothetical protein/rhs repeat‑associated core domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (20/32); new base A (2/2); total (22/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01 | |||||||||||
GCGCAGAGCCTGTTGCTGATGAACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCATTGCTCCGTGTTGAAGTACAATG > NC_002947/3578282‑3578575 | gcgcAGAGCCTGTTGCTGATGAACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCAttt > 8:260112/1‑149 (MQ=255) gcAGAGCCTGTTGCTGATGAACTTCCTGCCGGTGCAGTAAGAGGCCGCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCAttttt > 2:148075/1‑149 (MQ=255) cAGAGCCTGTTGCTGATGAACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTg < 8:8876/149‑1 (MQ=255) agagCCTGTTGCTGATGAACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGc < 5:125658/149‑1 (MQ=255) gtgatgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCAAAAATGCCAGTTGCAGc < 8:277905/147‑1 (MQ=255) tgatgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACAGGGATTTTTTGCAGTTGGAGCg > 4:577/1‑148 (MQ=255) tgatgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCAAAAAAGAGTTTCGTCGGGCTGCAACAGGGATTTTTTGCAGtttgagc > 1:147699/1‑142 (MQ=255) gatgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAg > 7:282174/1‑149 (MQ=255) atgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGCGCGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAg > 1:284992/1‑149 (MQ=255) atgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGa < 4:293089/147‑1 (MQ=255) atgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAg < 7:183895/148‑1 (MQ=255) atgaACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGACCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCAATTTTGACAGTTGCAGCGa > 6:434844/1‑147 (MQ=255) aaCTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTa < 4:217303/149‑1 (MQ=255) aCTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCTTCGGGCTGCAACTTGCATTTTTGCCAGTTGCAGCGAGTGTAt < 6:142839/149‑1 (MQ=255) ccgtggCAGTAAGAGGCCTCTGC‑CGCAGGCCGTTTGCGAAATGCCCTTACTTGAATGTGTATTGTGTTGATTAGTGTTCTTTAGCGATTCAACGTTTCGTCTGGCTGAAACTGTCATTTTTGCCTGTTGCAGCGAGTGTATGACAGCCt < 8:347868/144‑1 (MQ=255) ccGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGc < 3:577/147‑1 (MQ=255) gTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTTCCAGTTGCAGCGAGTGTATGACAGCCTAg > 3:178231/1‑148 (MQ=255) cAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAAtt < 1:148075/149‑1 (MQ=255) gTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCa < 7:161052/149‑1 (MQ=255) gTAAGACGCCTGTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATTAGGATTGAGTTGATGAGTGTTCATTAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCa < 6:15208/149‑1 (MQ=255) atgagGCCTCTTC‑CGCACGCCCTTTCCTAAATGCCCATACATGAATGAGGATTGAGTTGATTAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCaa < 4:258341/146‑1 (MQ=255) aaGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCaa < 3:38548/148‑1 (MQ=255) agagGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAg < 8:316699/148‑1 (MQ=255) gacgCCGCTGC‑CGCACGCCCGTGGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAggg < 6:300706/146‑1 (MQ=255) gCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGGATTTTTTGCAGTTGCAGCGAGTGTATTAAAGCCGAGAATTAAAGGGaaa > 8:55974/1‑149 (MQ=255) gCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGaaa < 2:284992/149‑1 (MQ=255) cGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTTCAAATGGCATTTTTTGCAGTTGCAGCGAGTGTATGACAGCCTATAATTTAAGGGAAATgttgttgtat > 7:144374/1‑144 (MQ=255) ccTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAgg > 5:153949/1‑149 (MQ=255) tttGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGGTGCAACAGGCAATTTTTGCAGTTGCGACGGAGGTATTTCAGCCTAGAATTAAAGGGAAAATGTGTTATGTAAGGt > 6:40355/1‑148 (MQ=255) gCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCAt < 6:153949/148‑1 (MQ=255) gAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACAAGCATTTTTTCCAGTTGAAGCGAGTGTATGACAACCTACAATTCAAGTGAAAAGGTGTTATCTAAGGTCATTa > 4:334456/1‑148 (MQ=255) aaTGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATc < 1:14266/149‑1 (MQ=255) tGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGt < 3:334456/149‑1 (MQ=255) atgaatgaGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTc < 7:260112/149‑1 (MQ=255) atgaatgaGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACAGGCATTTTTTCCAGTTGCAGCGAGTGTATTAAAGCCTAGAATTCAAAGGAAATTGTTTTATATAAGGTCATTAATAGTGAAACGTTc > 2:122030/1‑149 (MQ=255) tgaatgaGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCg < 4:178231/149‑1 (MQ=255) aatgaGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGc < 1:436489/148‑1 (MQ=255) atgaGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTACGCaa < 8:282174/149‑1 (MQ=255) atgaGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTAAAAGGAAAATGTTTTGTGTAATGTCATTTATAGTGAAAAGTTCGCaa > 6:387225/1‑149 (MQ=255) aGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGc < 1:122030/148‑1 (MQ=255) ggATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGt < 7:286731/149‑1 (MQ=255) aGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTTCCAGTTTCAGCGAGTGTATGACAGCCTAGAATTAAAGGGAAATGGTGTTGTCTAAGGTAATTAATCGTGAAACGTTAGCAAGCGTaaga > 8:106420/1‑144 (MQ=255) aGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGt < 7:106420/143‑1 (MQ=255) gTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGcc > 5:184968/1‑149 (MQ=255) atgaGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGGATTTTTGCCAGTTGCAGCGAGTGTATGAAAGCCTAGAATTCAAGGGAAATGGTGTTATCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTAc > 5:194383/1‑149 (MQ=255) aGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGGATTTTTTGCAGTTTCAGCGAGTGTATTACAGCCTAGAATTAAAGGGAAATGGTGTTCTCTAAAGTCAATTATAGTGAAAAGTTCGCAAGCGTACACCCCCTACGgc > 8:48512/1‑149 (MQ=255) gtTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACAGGGGTTTTTGGCAGTTGGGGGGGGTGGTTGGCAGCGTAGAATTGAAGGGGAATGGGGTTGTGTAAGGGGATTAATGGTGGAACGTTGGGAAGAGTAGACGGCCGACGgcgcg > 8:72531/1‑148 (MQ=255) tGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTg < 5:40355/149‑1 (MQ=255) gCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAgg < 3:32751/77‑1 (MQ=255) tCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTggg < 6:202894/143‑1 (MQ=255) tCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTggg > 5:202894/1‑143 (MQ=255) cGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCa < 5:387225/149‑1 (MQ=255) tGCAACTGGCAAAAATGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCATTGCTCCGTGtt > 8:382159/1‑148 (MQ=255) gCAACTGGCAAAAATGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGtt < 5:127104/92‑1 (MQ=38) gCAACTGGCAAAAATGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGtt > 6:127104/1‑92 (MQ=38) aaCTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCATTGCTCCGTGTTGAAg < 6:184968/149‑1 (MQ=255) tGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCATTGCTCCGTGTTGAAGTa > 4:284398/1‑148 (MQ=255) aTTTTTGCCAGTTGCAGCGAGTGTTTGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTAATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCATTGCTCCGTGTTGAAGTACAATg < 3:170356/149‑1 (MQ=255) | GCGCAGAGCCTGTTGCTGATGAACTTCCTGCCGGTGCAGTAAGAGGCCTCTGC‑CGCACGCCCTTTGCGAAATGCCCATACATGAATGAGGATTGAGTTGATGAGTGTTCATGAGCGAATCAACGTTTCGTCGGGCTGCAACTGGCATTTTTGCCAGTTGCAGCGAGTGTATGACAGCCTAGAATTCAAGGGAAATGGTGTTCTCTAAGGTCATTAATCGTGAAACGTTCGCAAGCGTACACGCCCTACGGCGCTATTTGGGGTGATGGCCATTGCTCCGTGTTGAAGTACAATG > NC_002947/3578282‑3578575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |