Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,632,086	A→C	7.9%	E327A (GAG→GCG) ‡	PP_5590 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,632,086	0	A	C	7.9%	172.8 / 5.1	60	E327A (GAG→GCG) ‡	PP_5590	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/25);  new base C (3/2);  total (33/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

CCGACCCCGGCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGACACCTGGGCTGCC  >  NC_002947/3631938‑3632234                                                                                                                                                     |                                                                                                                                                     ccGACCCCGGCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGa                                                                                                                                                      <  2:164241/149‑1 (MQ=255) ccGACCCCGGCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGGTCACCGGTCGCCTCGACCGCTTCTAGTGGAAGCTTGTGGGCAATAAGGATATTTACATACCCTACAACGCCAACAAGGTGTTCACCGCCAGCAAAGCCGa                                                                                                                                                      <  8:232576/149‑1 (MQ=255)  cGACCCCGGCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAg                                                                                                                                                     >  1:185563/1‑149 (MQ=255)      cccGGCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACATGGTGTTCACCGCCAGCAACGCCGAGgcgc                                                                                                                                                 >  4:28633/1‑149 (MQ=255)          gCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAGCGCCTCGGCgttgct                                                                                                                                             <  4:30144/149‑1 (MQ=255)            cACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAGCGCCTCGGCGTTGCTgg                                                                                                                                           <  7:23532/149‑1 (MQ=255)             aCCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGCGGCGCTGCTGGc                                                                                                                                          >  8:386073/1‑149 (MQ=255)                       tCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCAc                                                                                                                                <  7:63424/149‑1 (MQ=255)                       tCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCAc                                                                                                                                <  2:185563/149‑1 (MQ=255)                           gAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTg                                                                                                                             >  6:100884/1‑148 (MQ=255)                              cTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAAcc                                                                                                                         <  5:294198/149‑1 (MQ=255)                               ttCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACCCCGAGGCGCTGCTGGCCCCCCATCACCTGAAccc                                                                                                                        >  1:283632/1‑149 (MQ=255)                                  gATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGa                                                                                                                     >  4:160097/1‑149 (MQ=255)                                   aTGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGAc                                                                                                                    >  1:228107/1‑149 (MQ=255)                                    tGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAAccc                                                                                                                        <  6:319796/144‑1 (MQ=255)                                    tGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAAccc                                                                                                                        >  5:319796/1‑144 (MQ=255)                                          gTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATcc                                                                                                             >  5:241763/1‑149 (MQ=255)                                           tACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATc                                                                                                              <  2:28866/147‑1 (MQ=255)                                              gtgtTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTg                                                                                                         >  8:90783/1‑149 (MQ=255)                                               tgtTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTgg                                                                                                        <  2:228107/149‑1 (MQ=255)                                               tgtTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTgg                                                                                                        <  1:88696/149‑1 (MQ=255)                                                 ttCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGa                                                                                                      <  5:100884/149‑1 (MQ=255)                                                    aaCGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCt                                                                                                   <  2:283632/149‑1 (MQ=255)                                                                gACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGtgtgtg                                                                                       <  7:90783/149‑1 (MQ=255)                                                                   cGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCACGACGCCATCCGCTGGGAGCTGCACCGTGTgtgggt                                                                                    >  4:147845/1‑149 (MQ=255)                                                                        cGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACACGGTGTTCACCGCCAGCACCGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGggtggt                                                                                 <  2:44216/147‑1 (MQ=255)                                                                           gTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGAc                                                                                                                    <  2:76736/109‑1 (MQ=255)                                                                           gTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGAc                                                                                                                    >  1:76736/1‑109 (MQ=255)                                                                           gTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTCAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAgg                                                                            <  6:87188/149‑1 (MQ=255)                                                                            tGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGCAGCTGCACCGTGTGTGGGTGGTGGAGGc                                                                           <  1:244384/149‑1 (MQ=255)                                                                                   tggtggGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCt                                                                     >  5:48213/1‑148 (MQ=255)                                                                                    ggtggGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTg                                                                    >  7:187456/1‑148 (MQ=255)                                                                                     gtggGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTgaa                                                                  <  8:187456/149‑1 (MQ=255)                                                                                     gtggGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTga                                                                   <  1:150550/148‑1 (MQ=255)                                                                                       gggCAAGAAGGAGATGTACATCCCCTACAACGCCAACATGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTgaaga                                                                <  3:28633/149‑1 (MQ=255)                                                                                        ggCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGAc                                                                                                                    >  8:210366/1‑96 (MQ=255)                                                                                        ggCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTgaaga                                                                <  1:247035/148‑1 (MQ=255)                                                                                         gCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAAt                                                              >  1:42337/1‑149 (MQ=255)                                                                                          cAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATg                                                             >  1:400458/1‑149 (MQ=255)                                                                                          cAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGCTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGCGTGTGGGTGGTGGAGGCGACGCTGAAGAATg                                                             >  1:297880/1‑149 (MQ=255)                                                                                             gaagGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGcc                                                          <  4:92466/149‑1 (MQ=255)                                                                                              aagGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGc                                                           >  1:301312/1‑147 (MQ=255)                                                                                                ggAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAgc                                                       >  7:137094/1‑149 (MQ=255)                                                                                                         cATCCCCTACAACGCCAACAAGCTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTTAACACCGACGCCAACCGCTGGGAGCTGAACCGTGTGTGGGTGGTGGAGTCGACGATGAAGAATTGCCAGCGCCATCTGa                                              >  3:79167/1‑149 (MQ=255)                                                                                                           tCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATg                                            >  6:189657/1‑149 (MQ=255)                                                                                                           tCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACCCCGAGCCGCTGCTGGCCCCCCATCACCTGAACACCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCACCGCCATCTGaag                                            >  5:21328/1‑147 (MQ=255)                                                                                                             cccTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGAACCGTGTGTGGGTGGTGGAGGCGAAGCTGAAGAATGGACATCGCCATGTGATGcc                                          >  2:254928/1‑149 (MQ=255)                                                                                                                  cAACGCCAACTAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGt                                      <  6:21328/148‑1 (MQ=255)                                                                                                                  cAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCcgcg                                       <  6:241763/147‑1 (MQ=255)                                                                                                                  cAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGt                                      <  4:123898/148‑1 (MQ=255)                                                                                                                            aaGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACctacta                           >  6:193719/1‑149 (MQ=255)                                                                                                                                  ttCACCGCCAGCAACGCCAAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTcga                     >  5:110141/1‑149 (MQ=255)                                                                                                                                    cACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTcgac                    >  4:395534/1‑148 (MQ=255)                                                                                                                                     aCCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTcgacga                  <  3:160097/149‑1 (MQ=255)                                                                                                                                      ccGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGaa                 <  7:386073/149‑1 (MQ=255)                                                                                                                                       cGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAg                >  8:91554/1‑149 (MQ=255)                                                                                                                                         ccAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGac              <  2:289758/149‑1 (MQ=255)                                                                                                                                          cagcagCGCCTCGGCGTTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTCGCACCTACTACCTCGACGAAGaca             >  5:230067/1‑149 (MQ=255)                                                                                                                                           aGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGacac            >  5:61189/1‑149 (MQ=255)                                                                                                                                            gCAGCGCCTCGGCGTTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGACAcc           >  6:258293/1‑149 (MQ=255)                                                                                                                                              aaCGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGACACCTg         >  3:344582/1‑149 (MQ=255)                                                                                                                                                  ccGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGACACCTGGGCt     >  7:34946/1‑149 (MQ=255)                                                                                                                                                     aGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGACACCTGGGCTGcc  <  2:301312/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCGACCCCGGCCACCGCCGGCGTCATGAGCTTCGATGAGCTGTACGTGTTCAACGGTCGCCTCGACCGCTTCGAGTGGAAGCTGGTGGGCAAGAAGGAGATGTACATCCCCTACAACGCCAACAAGGTGTTCACCGCCAGCAACGCCGAGGCGCTGCTGGCCCCCCATCACCTGAACCCCGACGCCATCCGCTGGGAGCTGCACCGTGTGTGGGTGGTGGAGGCGACGCTGAAGAATGGCCAGCGCCATGTGATGCCGCGTAGCACCTACTACCTCGACGAAGACACCTGGGCTGCC  >  NC_002947/3631938‑3632234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑