Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,653,195	C→A	5.9%	Q361K (CAG→AAG) ‡	PP_3218 →	NtaA/SnaA/SoxA family monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,653,195	0	C	A	5.9%	217.1 / 3.1	68	Q361K (CAG→AAG) ‡	PP_3218	NtaA/SnaA/SoxA family monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (28/36);  new base A (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

TTTCAGCCAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTAC  >  NC_002947/3653050‑3653339                                                                                                                                                   |                                                                                                                                                 tttCAGCCAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGt                                                                                                                                               >  6:103484/1‑149 (MQ=255)     acccAGTACGACCCTGCTGCCCCCTTCCCGCAAGAGAGCCTCGAGTCTGCACGGAGCACCTTTTTC‑ACGAATGCCAAGCGAATTGCCGACCAGGCGCGGGACAAGGCCTTCAGGCTGCCTCAGGCTGTTCACTTTGGCCCTCAgcaacg                                                                                                                                           <  4:127758/147‑6 (MQ=255)       ccAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGc                                                                                                                                         >  8:35728/1‑149 (MQ=255)             cGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTAACTGACGGCCaaact                                                                                                                                   <  3:69482/149‑5 (MQ=255)              gACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGc                                                                                                                                  >  7:150294/1‑149 (MQ=255)               aCCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGcc                                                                                                                                 >  7:195114/1‑149 (MQ=255)                ccTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCt                                                                                                                                >  1:378016/1‑149 (MQ=255)                 cTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgg                                                                                                                                     >  3:76500/1‑143 (MQ=255)                 cTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgg                                                                                                                                     <  4:76500/143‑1 (MQ=255)                     aTGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGtt                                                                                                                           <  4:316763/149‑1 (MQ=255)                      tGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGTCCGTCAGTTACGGCCAGGGGCCTTCGTTg                                                                                                                          >  7:64527/1‑149 (MQ=255)                        gcccGTTCACTCAAGAGAGCCTGTAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCAAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTgg                                                                                                                         <  8:367876/147‑1 (MQ=255)                         cccGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGtt                                                                                                                       <  5:103484/149‑1 (MQ=255)                               cccGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTCTCCCAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGc                                                                                                                   <  1:363977/148‑1 (MQ=255)                                ccGCAAGAGACCCTGGAGTTTGCACGGAGCAGCTTTTTC‑AGGAATGCCAAGCGTATTGCGGAGCAGGCGGGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCg                                                                                                                 <  3:270869/148‑1 (MQ=255)                                 cGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGa                                                                                                                >  6:292475/1‑148 (MQ=255)                                 cGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGa                                                                                                                >  2:432977/1‑148 (MQ=255)                                 cGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGa                                                                                                                >  5:429825/1‑148 (MQ=255)                                  gCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGaaa                                                                                                              <  7:35728/149‑1 (MQ=255)                                  gCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGaaa                                                                                                              >  8:26107/1‑149 (MQ=255)                                   cAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAAc                                                                                                             >  3:354561/1‑149 (MQ=255)                                       agagCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTg                                                                                                         <  8:64527/149‑1 (MQ=255)                                            cTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTg                                                                                                                          >  5:420600/1‑127 (MQ=255)                                            cTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTg                                                                                                                          <  6:420600/127‑1 (MQ=255)                                               gAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGa                                                                                                  <  5:180637/148‑1 (MQ=255)                                               gAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGa                                                                                                  <  1:377007/148‑1 (MQ=255)                                                  tttGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGa                                                                                               <  6:321237/148‑1 (MQ=255)                                                      cACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGcc                                                                                          <  4:206630/149‑1 (MQ=255)                                                      cACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGcc                                                                                          <  4:199736/149‑1 (MQ=255)                                                      cACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGcc                                                                                          <  2:378016/149‑1 (MQ=255)                                                                     tC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgggg                                                                                                                                   <  2:424675/93‑1 (MQ=255)                                                                     tC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgggg                                                                                                                                   >  1:424675/1‑93 (MQ=255)                                                                        aCGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAgcgc                                                                          <  7:343447/148‑1 (MQ=255)                                                                        aCGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGcgcg                                                                         >  6:371423/1‑149 (MQ=255)                                                                               ccAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTg                                                                  >  5:25531/1‑149 (MQ=255)                                                                                cgaGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTgg                                                                 <  5:407140/147‑1 (MQ=255)                                                                                 aaGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGa                                                                >  5:185733/1‑149 (MQ=255)                                                                                 aaGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGa                                                                >  8:234133/1‑149 (MQ=255)                                                                                   gCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATg                                                              <  6:182605/149‑1 (MQ=255)                                                                                   gCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATg                                                              >  5:182605/1‑149 (MQ=255)                                                                                     gTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATggg                                                            <  8:195114/149‑1 (MQ=255)                                                                                      tATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGt                                                           <  5:371423/149‑1 (MQ=255)                                                                                       aTTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGt                                                           <  8:16574/148‑1 (MQ=255)                                                                                          gCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCa                                                        <  5:292475/148‑1 (MQ=255)                                                                                              aCCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATc                                                   >  3:368712/1‑149 (MQ=255)                                                                                               ccAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCt                                                  <  1:432977/149‑1 (MQ=255)                                                                                                   gcgcGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACAt                                              >  6:399516/1‑149 (MQ=255)                                                                                                   gcgcGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTGGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACAt                                              <  3:365869/149‑1 (MQ=255)                                                                                                              ggCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTg                                   <  5:399516/149‑1 (MQ=255)                                                                                                                ccTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                 >  5:294824/1‑149 (MQ=255)                                                                                                                ccTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                 <  2:105809/149‑1 (MQ=255)                                                                                                                 cTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                 >  4:180229/1‑148 (MQ=255)                                                                                                                 cTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                 >  2:66735/1‑148 (MQ=255)                                                                                                                    cAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCa                               <  7:26107/147‑1 (MQ=255)                                                                                                                       tCTGCGTCAGGCTGTTGAGTTTGGCAGTCAGTTACGGCCAGGGGCCTTCGATGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCAAGATcc                          >  6:191808/1‑149 (MQ=255)                                                                                                                          gCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGa                                                                                        <  5:402773/84‑1 (MQ=255)                                                                                                                          gCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGa                                                                                        >  6:402773/1‑84 (MQ=255)                                                                                                                               aGGCTGTTGAGTTTGGCCGTCAGTTACTGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAatat                                                    <  8:31249/115‑1 (MQ=255)                                                                                                                               aGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGtt                   >  2:35299/1‑148 (MQ=255)                                                                                                                                 gCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTa                 <  4:354561/148‑1 (MQ=255)                                                                                                                                 gCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTAc                <  6:185733/149‑1 (MQ=255)                                                                                                                                     ttGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCa                                                        <  1:248744/105‑1 (MQ=255)                                                                                                                                     ttGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCa                                                        >  2:248744/1‑105 (MQ=255)                                                                                                                                        cccctGGCCGTAACTGACGGCCAGCGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCgaag           >  2:268135/5‑147 (MQ=255)                                                                                                                                         gTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGGTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGaagaag        <  6:25531/149‑1 (MQ=255)                                                                                                                                         ccctGGCCGTAACTGACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCgaagaa         <  5:249050/145‑1 (MQ=255)                                                                                                                                         ccctGGCCGTAACTGACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCgaag           >  6:249050/4‑146 (MQ=255)                                                                                                                                               ccGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTAc  <  6:429825/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 TTTCAGCCAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTAC  >  NC_002947/3653050‑3653339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑