Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,653,197 G→C 5.9% Q361H (CAG→CAC) ‡ PP_3218 → NtaA/SnaA/SoxA family monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,653,1970GC 5.9% 221.5 / 3.7 68Q361H (CAG→CAC) ‡PP_3218NtaA/SnaA/SoxA family monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (28/36);  new base C (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

TTTCAGCCAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTACCGTTG  >  NC_002947/3653050‑3653344
                                                                                                                                                    |                                                                                                                                                   
tttCAGCCAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGt                                                                                                                                                    >  6:103484/1‑149 (MQ=255)
    acccAGTACGACCCTGCTGCCCCCTTCCCGCAAGAGAGCCTCGAGTCTGCACGGAGCACCTTTTTC‑ACGAATGCCAAGCGAATTGCCGACCAGGCGCGGGACAAGGCCTTCAGGCTGCCTCAGGCTGTTCACTTTGGCCCTCAgcaacg                                                                                                                                                <  4:127758/147‑6 (MQ=255)
      ccAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGc                                                                                                                                              >  8:35728/1‑149 (MQ=255)
            cGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTAACTGACGGCCaaact                                                                                                                                        <  3:69482/149‑5 (MQ=255)
             gACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGc                                                                                                                                       >  7:150294/1‑149 (MQ=255)
              aCCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGcc                                                                                                                                      >  7:195114/1‑149 (MQ=255)
               ccTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCt                                                                                                                                     >  1:378016/1‑149 (MQ=255)
                cTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgg                                                                                                                                          >  3:76500/1‑143 (MQ=255)
                cTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgg                                                                                                                                          <  4:76500/143‑1 (MQ=255)
                    aTGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGtt                                                                                                                                <  4:316763/149‑1 (MQ=255)
                     tGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGTCCGTCAGTTACGGCCAGGGGCCTTCGTTg                                                                                                                               >  7:64527/1‑149 (MQ=255)
                       gcccGTTCACTCAAGAGAGCCTGTAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCAAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTgg                                                                                                                              <  8:367876/147‑1 (MQ=255)
                        cccGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGtt                                                                                                                            <  5:103484/149‑1 (MQ=255)
                              cccGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTCTCCCAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGc                                                                                                                        <  1:363977/148‑1 (MQ=255)
                               ccGCAAGAGACCCTGGAGTTTGCACGGAGCAGCTTTTTC‑AGGAATGCCAAGCGTATTGCGGAGCAGGCGGGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCg                                                                                                                      <  3:270869/148‑1 (MQ=255)
                                cGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGa                                                                                                                     >  6:292475/1‑148 (MQ=255)
                                cGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGa                                                                                                                     >  2:432977/1‑148 (MQ=255)
                                cGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGa                                                                                                                     >  5:429825/1‑148 (MQ=255)
                                 gCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGaaa                                                                                                                   <  7:35728/149‑1 (MQ=255)
                                 gCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGaaa                                                                                                                   >  8:26107/1‑149 (MQ=255)
                                  cAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAAc                                                                                                                  >  3:354561/1‑149 (MQ=255)
                                      agagCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTg                                                                                                              <  8:64527/149‑1 (MQ=255)
                                           cTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTg                                                                                                                               >  5:420600/1‑127 (MQ=255)
                                           cTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTg                                                                                                                               <  6:420600/127‑1 (MQ=255)
                                              gAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGa                                                                                                       <  5:180637/148‑1 (MQ=255)
                                              gAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGa                                                                                                       <  1:377007/148‑1 (MQ=255)
                                                 tttGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGa                                                                                                    <  6:321237/148‑1 (MQ=255)
                                                     cACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGcc                                                                                               <  4:206630/149‑1 (MQ=255)
                                                     cACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGcc                                                                                               <  4:199736/149‑1 (MQ=255)
                                                     cACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGcc                                                                                               <  2:378016/149‑1 (MQ=255)
                                                                    tC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgggg                                                                                                                                        <  2:424675/93‑1 (MQ=255)
                                                                    tC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAgggg                                                                                                                                        >  1:424675/1‑93 (MQ=255)
                                                                       aCGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAgcgc                                                                               <  7:343447/148‑1 (MQ=255)
                                                                       aCGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGcgcg                                                                              >  6:371423/1‑149 (MQ=255)
                                                                              ccAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTg                                                                       >  5:25531/1‑149 (MQ=255)
                                                                               cgaGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTgg                                                                      <  5:407140/147‑1 (MQ=255)
                                                                                aaGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGa                                                                     >  5:185733/1‑149 (MQ=255)
                                                                                aaGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGa                                                                     >  8:234133/1‑149 (MQ=255)
                                                                                  gCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATg                                                                   <  6:182605/149‑1 (MQ=255)
                                                                                  gCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATg                                                                   >  5:182605/1‑149 (MQ=255)
                                                                                    gTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATggg                                                                 <  8:195114/149‑1 (MQ=255)
                                                                                     tATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGt                                                                <  5:371423/149‑1 (MQ=255)
                                                                                      aTTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGt                                                                <  8:16574/148‑1 (MQ=255)
                                                                                         gCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCa                                                             <  5:292475/148‑1 (MQ=255)
                                                                                             aCCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATc                                                        >  3:368712/1‑149 (MQ=255)
                                                                                              ccAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCt                                                       <  1:432977/149‑1 (MQ=255)
                                                                                                  gcgcGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACAt                                                   >  6:399516/1‑149 (MQ=255)
                                                                                                  gcgcGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTGGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACAt                                                   <  3:365869/149‑1 (MQ=255)
                                                                                                             ggCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTg                                        <  5:399516/149‑1 (MQ=255)
                                                                                                               ccTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                      >  5:294824/1‑149 (MQ=255)
                                                                                                               ccTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                      <  2:105809/149‑1 (MQ=255)
                                                                                                                cTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                      >  4:180229/1‑148 (MQ=255)
                                                                                                                cTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGc                                      >  2:66735/1‑148 (MQ=255)
                                                                                                                   cAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCa                                    <  7:26107/147‑1 (MQ=255)
                                                                                                                      tCTGCGTCAGGCTGTTGAGTTTGGCAGTCAGTTACGGCCAGGGGCCTTCGATGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCAAGATcc                               >  6:191808/1‑149 (MQ=255)
                                                                                                                         gCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGa                                                                                             <  5:402773/84‑1 (MQ=255)
                                                                                                                         gCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGa                                                                                             >  6:402773/1‑84 (MQ=255)
                                                                                                                              aGGCTGTTGAGTTTGGCCGTCAGTTACTGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAatat                                                         <  8:31249/115‑1 (MQ=255)
                                                                                                                              aGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGtt                        >  2:35299/1‑148 (MQ=255)
                                                                                                                                gCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTa                      <  4:354561/148‑1 (MQ=255)
                                                                                                                                gCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTAc                     <  6:185733/149‑1 (MQ=255)
                                                                                                                                    ttGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCa                                                             <  1:248744/105‑1 (MQ=255)
                                                                                                                                    ttGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCa                                                             >  2:248744/1‑105 (MQ=255)
                                                                                                                                       cccctGGCCGTAACTGACGGCCAGCGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCgaag                >  2:268135/5‑147 (MQ=255)
                                                                                                                                        gTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGGTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGaagaag             <  6:25531/149‑1 (MQ=255)
                                                                                                                                        ccctGGCCGTAACTGACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCgaagaa              <  5:249050/145‑1 (MQ=255)
                                                                                                                                        ccctGGCCGTAACTGACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCgaag                >  6:249050/4‑146 (MQ=255)
                                                                                                                                              ccGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTAc       <  6:429825/149‑1 (MQ=255)
                                                                                                                                                   aGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTACCGTtg  <  7:234133/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTCAGCCAGTACGACCTGGATGCCCCGTTCCCGCAAGAGAGCCTGGAGTTTGCACGGAGCAGCTTTTTC‑ACGAATGCCAAGCGTATTGCCGACCAGGCGCGGGACAAGGCCTTCAGTCTGCGTCAGGCTGTTGAGTTTGGCCGTCAGTTACGGCCAGGGGCCTTCGTTGGTTCGGCCGAAACCGTGGCGGCGAAGATGGCCGAGTGGTTCGAAGCGCGGGCGCTGGATGGGTTCAATATCTACATTGGTCATCCTGGCCAGTTCCGGCGGTTTACCGAAGAAGTGGTACCGTTG  >  NC_002947/3653050‑3653344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑