Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_002947 | 3,717,845 | T→G | 10.0% | N250H (AAC→CAC) | paaG ← | 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_002947 | 3,717,845 | 0 | T | G | 10.0% | 226.5 / 7.8 | 76 | N250H (AAC→CAC) | paaG | 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase |
Reads supporting (aligned to +/- strand): ref base T (36/32); new base G (3/5); total (39/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.75e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGTGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTG > NC_002947/3717705‑3717992 | ggTGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCtt < 4:344895/149‑1 (MQ=255) ggccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTGAACGCCTTcat < 1:294330/149‑3 (MQ=255) gccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTcgcg < 5:166232/149‑1 (MQ=255) gccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTcgcg < 8:302827/149‑1 (MQ=255) gccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTcgcg > 7:198843/1‑149 (MQ=255) gccggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTcccg > 8:384640/1‑149 (MQ=255) cggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCgg > 3:4349/1‑148 (MQ=255) ggccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTa < 2:181638/149‑1 (MQ=255) gccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGc > 1:419921/1‑138 (MQ=255) gccTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGc < 2:419921/138‑1 (MQ=255) gggCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCt > 4:334990/1‑149 (MQ=255) cgcgACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCCGCGCCCCGTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCt < 5:117499/149‑1 (MQ=255) cgcgACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCt > 4:90784/1‑149 (MQ=255) aCCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTgcgc < 8:112184/149‑1 (MQ=255) gggCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTg > 4:191277/1‑149 (MQ=255) ggCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTg < 4:4349/148‑1 (MQ=255) cGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGc > 7:403439/1‑147 (MQ=255) gtgccGGCGCCATGGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAgg > 4:415955/4‑149 (MQ=255) ccGGCGCCATGGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGgcgc > 4:44029/1‑149 (MQ=255) cGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTa > 7:298879/1‑123 (MQ=255) cGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTa < 8:298879/123‑1 (MQ=255) cGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCt > 4:9951/1‑149 (MQ=255) aTGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGGGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTCCCAGGCTCTGCAGGAcg > 8:360830/1‑149 (MQ=255) ggCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTcgcg > 3:264203/1‑149 (MQ=255) gCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCgctccagc > 7:46649/1‑149 (MQ=255) ccGATCCCCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCgctccagctc > 2:281062/1‑148 (MQ=255) aTCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCtccagctccagc > 3:155705/1‑149 (MQ=255) aTCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCtccagctccagc > 4:334944/1‑149 (MQ=255) aCCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTgc < 1:83187/149‑1 (MQ=255) ccGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTgcg > 6:12731/1‑149 (MQ=255) cGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTgcgc < 3:415955/149‑1 (MQ=255) cacaTTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCaaaa < 3:334990/147‑1 (MQ=255) aTTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCAtt < 8:82066/149‑1 (MQ=255) aTTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCAtt < 4:264203/149‑1 (MQ=255) ttgcttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTg < 8:198843/149‑1 (MQ=255) ttcttgcGAGTGCGGCCATTTTCAGCGCCCCTTGACCTCTGGCGCGCGTGTGTGCATGTATCCGTGCTCTCCCTTGCGGGTTTCCTCCGGCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCAtt < 1:343649/147‑1 (MQ=255) ttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTcg < 4:398028/112‑1 (MQ=255) ttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCg > 8:353741/1‑148 (MQ=255) ttgcGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCg > 8:203346/1‑148 (MQ=255) gTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTgg < 4:412755/149‑1 (MQ=255) ggCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCAtt < 3:90784/149‑1 (MQ=255) ggCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCAtt > 6:238066/1‑149 (MQ=255) ggCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCAtt < 5:189874/149‑1 (MQ=255) cATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAg > 1:116625/1‑149 (MQ=255) ttCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTg > 2:8173/1‑149 (MQ=255) aGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTgcgc < 3:9951/149‑1 (MQ=255) gcgcCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCt > 8:279932/1‑149 (MQ=255) cccTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGAt > 1:244835/1‑149 (MQ=255) cTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCa < 1:340472/149‑1 (MQ=255) gAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAgt < 7:384640/148‑1 (MQ=255) aaGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAgtgt < 5:12731/149‑1 (MQ=255) gCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAg > 7:339160/1‑149 (MQ=255) gcgtgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTgg > 8:192386/1‑117 (MQ=255) gcgtgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTgg < 7:192386/117‑1 (MQ=255) cgtgcgtTTGTTCATGAAGGCGTTCTCGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGt < 7:360830/149‑1 (MQ=255) gtgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCg > 8:22221/1‑147 (MQ=255) tgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAg < 2:116625/149‑1 (MQ=255) tgcgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAg < 3:44029/149‑1 (MQ=255) cgtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGGTCAGTGTCAGGTCGTAGGt < 8:403439/149‑1 (MQ=255) gtTTGTTCATGATGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGt < 3:239975/148‑1 (MQ=255) gtTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGt > 4:239975/1‑148 (MQ=255) ttGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGtggg > 4:307020/1‑149 (MQ=255) gcgAAGGCGTGAACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCa > 3:167324/3‑84 (MQ=255) gcgAAGGCGTGAACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCa < 4:167324/82‑1 (MQ=255) aTGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCACCTCCATTGTCGATAGTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGtgggctggg < 1:281062/148‑1 (MQ=255) cgAAGGCGTGAACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCgcgc < 6:418682/61‑1 (MQ=255) cgAAGGCGTGAACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTg > 6:442075/2‑119 (MQ=255) cgAAGGCGTGAACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTg < 5:442075/118‑1 (MQ=255) cgAAGGCGTGAACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCAGCCAGGCGCTGCAGGTCgcgc > 5:418682/2‑62 (MQ=38) aGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGggcggc > 3:218465/1‑148 (MQ=255) aGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGgcggcg > 7:362867/1‑149 (MQ=255) ggCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGa < 8:46649/149‑1 (MQ=255) gTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGtgggctgg < 8:112657/139‑1 (MQ=255) gTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGtgggctgg > 7:112657/1‑139 (MQ=255) gTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGt > 7:43690/1‑149 (MQ=255) gTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGt > 1:248528/1‑149 (MQ=255) ttCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTg > 4:140494/1‑149 (MQ=255) | GGTGGCCGGCCTGGGCCGCGACCTGGGCGATACCGGCGCCCATGGCGCCGGCACCGATCACCGCCACCTGCACATTGCTTGCGAGTGCGGCCATTTTCAGCGCCCCTTGAAGGCTGGCGTGCGTTTGTTCATGAAGGCGTTCACGCCTTCGCGGTAGTCCTC‑‑GCTGCGCCCTGCCAGGCGCTGCAGGTCGCGCTCCAGCTCCAGCTGCGCCTCAAAACCATTGTCGAAACTGGCATTGAGGCTGCGCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTG > NC_002947/3717705‑3717992 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |