Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,804,019 T→C 7.9% intergenic (‑103/‑41) PP_3361 ← / → PP_3362 aminotransferase/AsnC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,804,0190TC 7.9% 173.8 / 4.9 64intergenic (‑103/‑41)PP_3361/PP_3362aminotransferase/AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (34/24);  new base C (2/3);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GCTTGCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAG  >  NC_002947/3803875‑3804163
                                                                                                                                                |                                                                                                                                                
gCTTGCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGa                                                                                                                                              >  3:86339/1‑149 (MQ=255)
 cTTGCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGAc                                                                                                                                             <  7:393762/149‑1 (MQ=255)
  ttGCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGAcc                                                                                                                                            >  5:391143/1‑149 (MQ=255)
  ttGCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCACCGAAACGGACACACTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGAcc                                                                                                                                            >  1:259759/1‑149 (MQ=255)
           aCCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTCGGAACCGGCATAATg                                                                                                                                   <  5:107604/149‑1 (MQ=255)
             cGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTCGGAACCGGCATAATgcc                                                                                                                                 <  7:2921/149‑3 (MQ=255)
              gAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACg                                                                                                                                >  2:8888/1‑149 (MQ=255)
                 gTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGc                                                                                                                              >  6:199882/1‑148 (MQ=255)
                    ccGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAg                                                                                                                           <  1:8888/148‑1 (MQ=255)
                            gAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAg                                                                                                                  >  5:135969/1‑149 (MQ=255)
                                 gTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCt                                                                                                             >  1:176840/1‑149 (MQ=255)
                                      tCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAAt                                                                                                        <  6:135969/149‑1 (MQ=255)
                                         tatCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCg                                                                                                     >  1:111668/1‑149 (MQ=255)
                                           tcatcaGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATAAAggg                                                                                                                >  6:415392/1‑136 (MQ=255)
                                             atcaGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGa                                                                                                  <  3:421556/148‑1 (MQ=255)
                                              tcaGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGTGGCTGCAATGCGTGAcc                                                                                                >  4:132650/1‑149 (MQ=255)
                                              tcaGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTGCCGACAGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCgggccc                                                                                                >  4:329306/1‑144 (MQ=255)
                                                aGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCt                                                                                               <  5:166288/148‑1 (MQ=255)
                                                aGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCt                                                                                               <  2:111668/148‑1 (MQ=255)
                                                 ggTTCCGAGCTCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTgg                                                                                             <  4:400901/149‑1 (MQ=255)
                                                  gTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGa                                                                                            >  2:257207/1‑149 (MQ=255)
                                                       gAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCt                                                                                        >  4:55728/1‑148 (MQ=255)
                                                                 aTTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACCCCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGAACGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATcgc                                                                               >  6:439616/1‑147 (MQ=255)
                                                                  ttGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATcgc                                                                               >  7:90991/1‑146 (MQ=255)
                                                                  ttGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATcgc                                                                               >  1:54777/1‑146 (MQ=255)
                                                                   tGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGAt                                                                                  >  7:210342/1‑142 (MQ=255)
                                                                   tGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGAt                                                                                  <  8:210342/142‑1 (MQ=255)
                                                                       tttCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGATCTGGACGCTAAAGATCGCGAGATTc                                                                        <  2:176840/148‑1 (MQ=255)
                                                                         tCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCT‑AAGATCGCGAGATTCTTg                                                                     <  1:239674/148‑1 (MQ=255)
                                                                          cAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTg                                                                     <  2:54777/148‑1 (MQ=255)
                                                                           aaTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAg                                                                   <  8:7655/149‑1 (MQ=255)
                                                                           aaTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAg                                                                   <  5:439616/149‑1 (MQ=255)
                                                                            aTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGc                                                                                                           >  4:259975/1‑108 (MQ=255)
                                                                            aTAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGc                                                                                                           <  3:259975/108‑1 (MQ=255)
                                                                                   gTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGATCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACttt                                                            <  1:297364/148‑1 (MQ=255)
                                                                                          gccgccGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAc                                                    <  5:199882/149‑1 (MQ=255)
                                                                                              ccgAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAcgcgc                                                <  4:86339/149‑1 (MQ=255)
                                                                                                       acacCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTgcgc                                       >  5:168327/1‑149 (MQ=255)
                                                                                                         acCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCt                                      >  2:201959/1‑148 (MQ=255)
                                                                                                           ccTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTc                                     >  6:255174/1‑147 (MQ=255)
                                                                                                            cTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAg                                  >  7:63595/1‑149 (MQ=255)
                                                                                                              gCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGc                                >  4:29341/1‑149 (MQ=255)
                                                                                                               cATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGCACTGGACGCGAAAGATCGCGAGATTCTTGAGGGACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCg                               >  6:321646/1‑149 (MQ=255)
                                                                                                                    ggTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAgc                          <  3:55728/149‑1 (MQ=255)
                                                                                                                      tCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAgccg                        >  1:65513/1‑149 (MQ=255)
                                                                                                                         ggCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAgccggcc                     <  3:132650/149‑1 (MQ=255)
                                                                                                                          gcgcAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAgccggccg                    >  5:211832/1‑149 (MQ=255)
                                                                                                                                cAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAgg              >  7:336240/1‑149 (MQ=255)
                                                                                                                                 cggCATAATGCCTGTTGCGCCCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGc             >  7:324479/2‑149 (MQ=255)
                                                                                                                                 aGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGc             <  8:63595/149‑1 (MQ=255)
                                                                                                                                 aGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGc             >  7:335412/1‑149 (MQ=255)
                                                                                                                                  ggCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTgc                                         <  8:300965/120‑1 (MQ=255)
                                                                                                                                  ggCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTgc                                         >  7:300965/1‑120 (MQ=255)
                                                                                                                                  ggCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGcc            >  1:403015/1‑149 (MQ=255)
                                                                                                                                   gCATAATGCCTGTTGCGCCCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCt           >  5:85192/1‑149 (MQ=255)
                                                                                                                                      ttATGCCGGTCGGAACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCAt                 >  3:245988/1‑140 (MQ=255)
                                                                                                                                      ttATGCCGGTCGGAACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCAt                 <  4:245988/140‑1 (MQ=255)
                                                                                                                                       tATGCCGGTCGGAACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTcc       >  8:119974/1‑149 (MQ=255)
                                                                                                                                        aTGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTcc       <  5:255174/148‑1 (MQ=255)
                                                                                                                                           ccGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAcgcgc                                                <  1:153219/104‑1 (MQ=255)
                                                                                                                                           ccGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGAcgcgc                                                >  2:153219/1‑104 (MQ=255)
                                                                                                                                           ccGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGaa   <  2:259759/149‑1 (MQ=255)
                                                                                                                                           ccGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGGATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCGTACCCg     >  6:444926/1‑147 (MQ=255)
                                                                                                                                            cGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAg  >  6:2722/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCTTGCCGAAAACCGAGGTGCCGGGTGGGAAAGGTCTGTCATATCATCAGGTTCCGAGCGCGCTGATTGTTTTTCAATAGCTTGTCATGCGCCGCCGAAACGGACACCCTGCATACGGTCGGGCGCAACAGGCATTATGCCGGTTCCGACCGGCATAATGACGGCAAGCTTATCAAGGGGCTGCAATGCGTGACCTGGACGCTAAAGATCGCGAGATTCTTGAGGTACTTTCGAAGGACGCGCGTATTGCGCTCAAGGCGTTAGCCGGCCGCATAGGCCTTTCCCGAAG  >  NC_002947/3803875‑3804163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑