| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,882,813 | A→G | 10.5% | intergenic (+56/‑169) | oprN → / → PP_3428 | multidrug RND transporter outer membrane protein OprN/TPR repeat‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,882,813 | 0 | A | G | 10.5% | 142.0 / 8.0 | 57 | intergenic (+56/‑169) | oprN/PP_3428 | multidrug RND transporter outer membrane protein OprN/TPR repeat‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (27/24); new base G (2/4); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
AGGGTGAAGTCGACCTTTATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGCTCTGC > NC_002947/3882666‑3882956 | aGGGTGAAGTCGACCTTTATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAg > 2:15273/1‑149 (MQ=255) tGAAGTCGACCTTTATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCGGGcc > 6:6455/1‑148 (MQ=255) aaGTCGACCTTTATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCAt < 8:236141/148‑1 (MQ=255) gACCTTTATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCcg > 1:419639/1‑149 (MQ=255) tATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCGGGCCATGGCCTGCgggg < 5:384830/148‑1 (MQ=255) tATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCGGGCCATGGCCTGCggg < 5:6455/147‑1 (MQ=255) tATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCgggg < 7:94643/148‑1 (MQ=255) aTCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCGGGCCATGGCCTGCGGGGc < 5:89465/148‑1 (MQ=255) aTCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGc < 4:400771/148‑1 (MQ=255) aTCGAGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCt > 2:292068/1‑149 (MQ=255) gcgGCCTCGTCGCGCTCTACCAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCCCTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCtttt < 6:423893/149‑1 (MQ=255) cgGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCGGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCttttt < 7:245022/149‑1 (MQ=255) aTCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGTCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATg > 5:228264/1‑149 (MQ=255) aTCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGAGCACTGATTCCACTGCCTCAACGACTCCATTGGTTGGCTCCTCCCCCAACCGTTTTCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATg > 5:234158/1‑149 (MQ=255) tagTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCAAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGt < 2:110290/147‑1 (MQ=255) gTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGttt < 7:51360/149‑1 (MQ=255) tctcGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTg > 6:424883/1‑149 (MQ=255) cTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGACTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATct > 6:303303/1‑147 (MQ=255) tACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTa > 4:128248/1‑149 (MQ=255) cAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTAt > 6:8146/1‑148 (MQ=255) ggCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCAc < 3:26267/149‑1 (MQ=255) gCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCAcc < 2:419639/149‑1 (MQ=255) cTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTTGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCAcccc > 5:306381/1‑148 (MQ=255) gTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATAc > 3:404975/1‑149 (MQ=255) ggcTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTTGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGt < 6:306381/149‑1 (MQ=255) ggcTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGt > 2:264933/1‑149 (MQ=255) ggTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGcc < 2:277134/108‑1 (MQ=255) ggTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGcc > 1:277134/1‑108 (MQ=255) ggTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCg > 5:288461/1‑148 (MQ=255) tGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAg < 1:264933/149‑1 (MQ=255) gcgcgcACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGgcctgg > 1:241806/1‑149 (MQ=255) cgcgcACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGgcctggc < 1:292068/149‑1 (MQ=255) cTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGgcctgtcgcctgg < 5:303303/149‑1 (MQ=255) aTTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGgcctggcgcctggc < 5:424883/147‑1 (MQ=255) tCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAAc > 1:164487/1‑149 (MQ=255) aCTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGc > 4:363036/1‑149 (MQ=255) aCTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGGTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGTGCCTGGCCAACGGc < 1:15273/149‑1 (MQ=255) tCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGc > 2:42404/1‑143 (MQ=255) tCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGc < 1:42404/143‑1 (MQ=255) aCGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATCTATGCCATCGTATACACCCCAATAAGCAGTGCATGGAATGGCACCGCAGGCCTGGCGCCTGGCCAACGGCGTCCGcgga > 6:30183/1‑148 (MQ=255) gtcTCCTTTGGATGGGTCCTCGCCCATCCGTTTGCCGCGCAGGCCATGGCCCGCGGGGCTTTATTATGTTTGCGATCGTATTCACGCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCcggttc < 3:409710/147‑1 (MQ=255) tCCTTTGGTTGGCTCCTCCCCCACCCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCAACGTATTCCCCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCCACGGCGGGCGcggttcggt > 6:128770/1‑149 (MQ=255) cTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCcggttcggt < 3:128248/147‑1 (MQ=255) cTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCGTTTTTATGTTTGCCATCGTATTGCACCCAAAACGCAGTGCATGGACTGCCACCGCAGGCCTGGCGCCTGGCCACCGGCGTCCCCGGTTCGGGTg > 4:271823/1‑149 (MQ=255) gttggCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCAGCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCAttt < 8:122566/149‑1 (MQ=255) ggCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTTCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCCCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTAcc > 8:275372/1‑149 (MQ=255) tcctccCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCTCGGTTCGGTTGCATTTAccccc > 2:196888/1‑149 (MQ=255) cccAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTg > 1:252789/1‑149 (MQ=255) ccAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTgg < 2:241806/149‑1 (MQ=255) aaCCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGcc < 4:404975/149‑1 (MQ=255) ccGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGccc > 8:113932/1‑148 (MQ=255) gCCCCGCGGGCCATGGCCTGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCcggttcgg < 6:170978/118‑1 (MQ=255) gCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGctct > 2:332175/1‑149 (MQ=255) ccccGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTTGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGCTCTg < 1:332175/149‑1 (MQ=255) cccGCGGGCCATGGCCTGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGCTCTGc > 7:342211/1‑149 (MQ=255) cccGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGCTCTGc < 6:288461/149‑1 (MQ=255) cccGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGCTCTGc > 5:186996/1‑149 (MQ=255) | AGGGTGAAGTCGACCTTTATCGCGGCATCGTCTCGATCTACAAGGCCCTGGGTGGCGGCTGGCAACCGGAGACGGTGGCCAGCGCGCACTGATTCCACTGCCTCAACGACTCCTTTGGTTGGCTCCTCCCCCAACCGTTTGCCCCGCAGGCCATGGCCCGCGGGGCTTTTTTATGTTTGCCATCGTATTCACCCCAATACGCAGTGCATGGAATGCCACCGCAGGCCTGGCGCCTGGCCAACGGCGTGCCCGGTTCGGTTGCATTTACCCCCCGGCTGGCCCAAGCTCTGC > NC_002947/3882666‑3882956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |