Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 295,246 G→A 5.6% G217E (GGG→GAG)  ssuB → aliphatic sulfonates ABC transporter ATP‑binding protein SsuB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947295,2460GA 5.6% 328.2 / 4.9 107G217E (GGG→GAG) ssuBaliphatic sulfonates ABC transporter ATP‑binding protein SsuB
Reads supporting (aligned to +/- strand):  ref base G (56/45);  new base A (3/3);  total (59/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01

Only 100 of 109 total aligned reads displayed.

CTGGATGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCAA  >  NC_002947/295098‑295391
                                                                                                                                                    |                                                                                                                                                 
cTGGATGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCgg                                                                                                                                                   <  5:295447/149‑1 (MQ=255)
   gATGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCggg                                                                                                                                                  <  8:248638/147‑1 (MQ=255)
   gATGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGc                                                                                                                                                 >  7:329983/1‑148 (MQ=255)
     tGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCg                                                                                                                                              <  1:238922/149‑1 (MQ=255)
     tGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCCAGGTCGGGCTc                                                                                                                                               >  3:221788/1‑148 (MQ=255)
      gCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGa                                                                                                                                             >  4:186489/1‑149 (MQ=255)
        gTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGAcc                                                                                                                                           >  6:185426/1‑149 (MQ=255)
        gTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGAcc                                                                                                                                           <  4:128950/149‑1 (MQ=255)
            aCCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGAGCCCGACCTcg                                                                                                                                        <  6:402952/148‑2 (MQ=255)
             cccTTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGAGCCCGACCTcgcc                                                                                                                                      <  4:299151/149‑4 (MQ=255)
             cccGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGAGCCCGACCTcgcc                                                                                                                                      <  8:315032/149‑4 (MQ=255)
              ccGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTg                                                                                                                                     >  1:392877/1‑149 (MQ=255)
                   tCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGa                                                                                                                                 <  6:178178/148‑1 (MQ=255)
                   tCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGa                                                                                                                                 <  7:42163/148‑1 (MQ=255)
                      agaTGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTg                                                                                                                             >  7:169516/1‑149 (MQ=255)
                         tGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCa                                                                                                                          >  2:202420/1‑149 (MQ=255)
                          gcagcaACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCAc                                                                                                                         >  2:344382/1‑149 (MQ=255)
                           cagcaACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACg                                                                                                                        <  8:41913/149‑1 (MQ=255)
                            agcaACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACgg                                                                                                                       >  5:72196/1‑149 (MQ=255)
                               aaCTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGccc                                                                                                                    >  1:48796/1‑149 (MQ=255)
                                       gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGgcgc                                                                                                             <  8:169516/148‑1 (MQ=255)
                                       gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGcgcg                                                                                                            >  6:253558/1‑149 (MQ=255)
                                       gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGcgcg                                                                                                            >  5:50113/1‑149 (MQ=255)
                                       gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGcgcg                                                                                                            <  2:304043/149‑1 (MQ=255)
                                           gCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGt                                                                                                        >  3:203115/1‑149 (MQ=255)
                                           gCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGt                                                                                                        <  1:202420/149‑1 (MQ=255)
                                           gCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGt                                                                                                        >  4:219694/1‑149 (MQ=255)
                                             cTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTc                                                                                                      <  8:229725/149‑1 (MQ=255)
                                              tgtgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGt                                                                                                           >  6:235541/1‑143 (MQ=255)
                                              tgtgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGt                                                                                                           <  5:235541/143‑1 (MQ=255)
                                                tgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                   <  2:247350/149‑1 (MQ=255)
                                                  gCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCAcc                                                                                                 >  5:25395/1‑149 (MQ=255)
                                                    gTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGt                                                                                               <  5:253558/149‑1 (MQ=255)
                                                     tCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTc                                                                                              >  1:203155/1‑149 (MQ=255)
                                                        gCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTgg                                                                                           <  6:364728/149‑1 (MQ=255)
                                                         cACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGc                                                                                          <  3:186489/149‑1 (MQ=255)
                                                         cACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGc                                                                                          >  4:103382/1‑149 (MQ=255)
                                                          aCGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCAcc                                                                                                 <  2:128105/141‑1 (MQ=255)
                                                          aCGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCAcc                                                                                                 >  1:128105/1‑141 (MQ=255)
                                                          aCGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGcc                                                                                         >  2:318276/1‑149 (MQ=255)
                                                            ggCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCcgc                                                                                       <  2:48796/149‑1 (MQ=255)
                                                            ggCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCcgc                                                                                       >  8:302618/1‑149 (MQ=255)
                                                               ttCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCt                                                                                    >  8:62416/1‑149 (MQ=255)
                                                                 cACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACAGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTg                                                                                   >  3:163378/1‑148 (MQ=255)
                                                                  aCTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTgg                                                                                  >  2:178786/1‑148 (MQ=255)
                                                                  aCTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTgg                                                                                  <  6:50113/148‑1 (MQ=255)
                                                                      tACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGaaa                                                                               <  1:318276/147‑1 (MQ=255)
                                                                      tACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGc                                                                             >  6:379298/1‑149 (MQ=255)
                                                                      tACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGc                                                                             >  4:115074/1‑149 (MQ=255)
                                                                        ctgctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCg                                                                            <  5:185426/148‑1 (MQ=255)
                                                                         tgctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCg                                                                            >  8:252897/1‑147 (MQ=255)
                                                                           ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                          <  2:203155/147‑1 (MQ=255)
                                                                           ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAg                                                                         <  8:329983/148‑1 (MQ=255)
                                                                           ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGt                                                                        >  1:413195/1‑149 (MQ=255)
                                                                           ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGt                                                                        >  6:344850/1‑149 (MQ=255)
                                                                            tgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTg                                                                       >  6:442013/1‑149 (MQ=255)
                                                                              gTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                          <  3:242947/144‑1 (MQ=255)
                                                                                cACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTc                                                                    >  6:290242/1‑148 (MQ=255)
                                                                                    cACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCg                                                               <  4:203115/149‑1 (MQ=255)
                                                                                     acgacgTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCgt                                                              >  4:259519/1‑149 (MQ=255)
                                                                                           tCAGCGTGTCGGTTGCCGTGGCTGACCGGGTGATCCTGTTCGAGGACGGCGAGGTCGGGCTCGACGTGACTGTTGACCTGGGACGGCGGCTGGGGCGTGGTTCGGACCGTCTGGGCGCGCTTGAAAGAGAAGTGCTCAACGGTGTTCtt                                                        <  2:414481/149‑2 (MQ=255)
                                                                                           tCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTg                                                        <  4:163378/149‑1 (MQ=255)
                                                                                           tCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGGGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTg                                                        <  8:353437/149‑1 (MQ=255)
                                                                                                gAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAAcc                                                                <  5:173771/136‑1 (MQ=255)
                                                                                                gAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAAcc                                                                >  6:173771/1‑136 (MQ=255)
                                                                                                gAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCAc                                                   >  1:22379/1‑149 (MQ=255)
                                                                                                        tGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCCTGTTCTGTCCACCCCGGGCa                                           >  2:195173/1‑149 (MQ=255)
                                                                                                         gCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCAc                                          >  7:196927/1‑149 (MQ=255)
                                                                                                           cGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTg                                        >  4:269712/1‑149 (MQ=255)
                                                                                                            gTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTgc                                       >  3:197265/1‑149 (MQ=255)
                                                                                                             tGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTgcg                                      >  8:376871/1‑149 (MQ=255)
                                                                                                              ggCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTgcgc                                     >  1:377063/1‑149 (MQ=255)
                                                                                                                 tGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGccc                                   >  7:5775/1‑148 (MQ=255)
                                                                                                                  gACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGccc                                   >  4:148293/1‑147 (MQ=255)
                                                                                                                   aCCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                   >  5:353797/1‑82 (MQ=255)
                                                                                                                   aCCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                   <  6:353797/82‑1 (MQ=255)
                                                                                                                    ccGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGc                               <  5:379298/149‑1 (MQ=255)
                                                                                                                     cGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGcc                              <  7:62416/149‑1 (MQ=255)
                                                                                                                         tGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGAt                          >  5:379487/1‑149 (MQ=255)
                                                                                                                           aTCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATcc                        >  4:14740/1‑149 (MQ=255)
                                                                                                                           aTCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATcc                        >  5:36133/1‑149 (MQ=255)
                                                                                                                             ccTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCCGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTg                      >  5:155739/1‑149 (MQ=255)
                                                                                                                              cTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTg                      <  6:379487/148‑1 (MQ=255)
                                                                                                                              cTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTg                      >  7:54022/1‑148 (MQ=255)
                                                                                                                                gATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                          >  2:79121/1‑94 (MQ=255)
                                                                                                                                  tCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGcc                 >  4:124644/1‑149 (MQ=255)
                                                                                                                                    gAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGccc                <  1:344382/148‑1 (MQ=255)
                                                                                                                                     aGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAg                   >  7:376258/1‑144 (MQ=255)
                                                                                                                                     aGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAg                   <  8:376258/144‑1 (MQ=255)
                                                                                                                                        aCGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTg           <  4:197265/149‑1 (MQ=255)
                                                                                                                                         cagtgAGGTCGAGCCCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGc          >  8:106271/5‑149 (MQ=255)
                                                                                                                                          ggCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGcc         >  1:85605/1‑149 (MQ=255)
                                                                                                                                          ggCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGcc         <  6:318551/149‑1 (MQ=255)
                                                                                                                                           gtcaGGTCGAGCCCGAACTCGCTGTTGACCTGGCACGGCCCCGAGCGCGAGGTTCGCACAGTCTGGCCGCCCTGGAAAGCGAAGTGCTCACCCGTGTTCAGTCAACCCCGGGCACTGCGCCCCAGCCGGATCCTGTAGACACTATGccc        >  4:85944/4‑149 (MQ=255)
                                                                                                                                           gCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGcc                              <  2:203782/127‑1 (MQ=255)
                                                                                                                                           gCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGcc                              >  1:203782/1‑127 (MQ=255)
                                                                                                                                            tgAGGTCGAGCCCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGGCCa       >  6:51176/2‑149 (MQ=255)
                                                                                                                                               ggTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGc    <  7:376871/149‑1 (MQ=255)
                                                                                                                                                gTCGGGCTCGACCTGACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGc    <  3:219694/148‑1 (MQ=255)
                                                                                                                                                 tCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCaa  <  3:124644/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGGATGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCAA  >  NC_002947/295098‑295391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑