Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,915,472 G→C 5.7% K147N (AAG→AAC)  PP_3455 → multidrug RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,915,4720GC 5.7% 211.4 / 3.5 70K147N (AAG→AAC) PP_3455multidrug RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (38/28);  new base C (2/2);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

GCCGTTCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGC  >  NC_002947/3915328‑3915620
                                                                                                                                                  |                                                                                                                                                    
gcCGTTCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCa                                                                                                                                                  <  6:252207/149‑1 (MQ=255)
  cGTTCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGt                                                                                                                                                >  6:246596/1‑149 (MQ=255)
    ttCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGttt                                                                                                                                              >  6:121674/1‑149 (MQ=255)
    ttCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGttt                                                                                                                                              >  7:295087/1‑149 (MQ=255)
     tCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTc                                                                                                                                             >  6:137512/1‑149 (MQ=255)
         ggCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGc                                                                                                                                         <  5:121674/149‑1 (MQ=255)
          gCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAGTTCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGc                                                                                                                                         <  1:90086/149‑1 (MQ=255)
          gCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTg                                                                                                                                          >  2:82122/1‑147 (MQ=255)
             gACCATGACAGCGCCCGCGCCACACTGGCCAAGGCCGGAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAgg                                                                                                                                      >  1:78094/1‑149 (MQ=255)
               ccATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCg                                                                                                                                   <  2:78094/149‑1 (MQ=255)
                   gACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATg                                                                                                                                >  7:61400/1‑148 (MQ=255)
                   gACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGc                                                                                                                               >  8:219360/1‑149 (MQ=255)
                    aCAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGc                                                                                                                               <  1:82122/148‑1 (MQ=255)
                     cAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCg                                                                                                                             <  5:137512/149‑1 (MQ=255)
                      aGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACCACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCg                                                                                                                             >  7:238817/1‑148 (MQ=255)
                          cccGCGCCACCCTGGCCAAGGCC‑GCAGCCACCCGGTCCCAGGCGCGCCTGCAAGAGCAGCGCTCCCGCGAGCTGGTCGCCGACAAAG‑CCGTCAGCCGCCAGGCATACGCCACCGCCCAGGCCCGGTTCCTGCCGGCCGATGCCGAAGTg                                                                                                                        >  7:372532/1‑149 (MQ=255)
                            cgcgCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCgccctggc                                                                                                                      >  3:302190/1‑142 (MQ=255)
                                ccACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGaa                                                                                                                  <  2:394110/149‑1 (MQ=255)
                                     cTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAg                                                                                                             <  3:164894/149‑1 (MQ=255)
                                          cAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGggcgg                                                                                                        >  8:317496/1‑149 (MQ=255)
                                             ggCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGgcgc                                                                                                     <  6:55078/149‑1 (MQ=255)
                                              gCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCt                                                                                                    >  6:53703/1‑149 (MQ=255)
                                              gCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCt                                                                                                    <  4:282931/149‑1 (MQ=255)
                                                  gAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGa                                                                                                 <  1:376833/149‑1 (MQ=255)
                                                  gAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGa                                                                                                 <  8:61400/149‑1 (MQ=255)
                                                    aGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACAACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAgc                                                                                               >  4:82302/1‑149 (MQ=255)
                                                    aGCCACCCGGTACCAGGCGCGCCTACAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAgc                                                                                               <  2:124779/149‑1 (MQ=255)
                                                       cACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGAGGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAgcgcg                                                                                            >  6:330262/1‑149 (MQ=255)
                                                       cACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAgcgcg                                                                                            >  8:265272/1‑149 (MQ=255)
                                                        aCCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAgcgcgc                                                                                           >  6:310457/1‑149 (MQ=255)
                                                           cGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGC‑GCGCTCCAgcgccgccct                                                                                       <  7:39250/149‑7 (MQ=255)
                                                             gTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGc                                                                                      <  5:246596/149‑1 (MQ=255)
                                                               aCCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCt                                                                                     >  4:197187/1‑148 (MQ=255)
                                                                  aGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGccc                                                                                         >  6:280751/1‑141 (MQ=255)
                                                                  aGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGccc                                                                                         <  5:280751/141‑1 (MQ=255)
                                                                  aGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAAc                                                                                 <  5:53703/149‑1 (MQ=255)
                                                                        gcCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGc                                                                           >  8:386677/1‑149 (MQ=255)
                                                                             cAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAggg                                                                                                           >  6:102509/1‑112 (MQ=255)
                                                                             cAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAggg                                                                                                           <  5:102509/112‑1 (MQ=255)
                                                                              aaGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGc                                                                      >  6:323385/1‑148 (MQ=255)
                                                                               agagCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGCATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCa                                                                    <  7:228420/149‑1 (MQ=255)
                                                                                   cagcTCTACCGGGAGCTGGTCGACGACAAAG‑CGGTCAGCCGCCATGATTACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGggcg                                                                                                         <  8:247505/108‑1 (MQ=255)
                                                                                   cagcGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCAGCCAGGAATACGACAACGCCAAGGACAGGTTCCTGCAGGCAGATGCAGAAGTGGCCGAAGCCAGggcg                                                                                                         >  7:247505/1‑108 (MQ=255)
                                                                                    agcGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAACGCCAGTTTCCTGCAGGCCGCTGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTg                                                               >  6:436770/1‑149 (MQ=255)
                                                                                    agcGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAACGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGt                                                                >  6:430893/1‑148 (MQ=255)
                                                                                     gcgcTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGa                                                              <  2:324507/149‑1 (MQ=255)
                                                                                         tACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACcgc                                                          >  4:302492/1‑149 (MQ=255)
                                                                                                     gTCGACGACAACG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTTGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGTCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCg                                               <  8:150784/148‑1 (MQ=255)
                                                                                                     gTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCgg                                              >  7:339264/1‑149 (MQ=255)
                                                                                                     gTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCgg                                              <  5:55269/149‑1 (MQ=255)
                                                                                                        gacgacAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGcc                                            <  7:265272/148‑1 (MQ=255)
                                                                                                        gacgacAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCg                                           >  4:416010/1‑149 (MQ=255)
                                                                                                             cAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCg                                      >  2:67781/1‑149 (MQ=255)
                                                                                                              aaaG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGCCCGAAGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCgg                                     >  5:84581/1‑149 (MQ=255)
                                                                                                              aaaG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGCTTCCTGCAGGCCGATGCCAAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCgg                                     >  5:138424/1‑149 (MQ=255)
                                                                                                                    cGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCcgcg                                >  4:298811/1‑149 (MQ=255)
                                                                                                                        aGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCa                            >  8:43129/1‑149 (MQ=255)
                                                                                                                           cgccAGGAATACGACAACGCCAACGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGt                         <  5:436770/149‑1 (MQ=255)
                                                                                                                           cgccAGGAATACGACAACGCCAACGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGt                         <  5:430893/149‑1 (MQ=255)
                                                                                                                               aGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc                     <  5:323385/149‑1 (MQ=255)
                                                                                                                               aGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc                     >  3:394957/1‑149 (MQ=255)
                                                                                                                                ggAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCAcc                     >  5:307738/1‑148 (MQ=255)
                                                                                                                                   aTACGCCAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGGGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAg                 <  8:372532/149‑1 (MQ=255)
                                                                                                                                   aTACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAg                 <  7:317496/149‑1 (MQ=255)
                                                                                                                                   aTACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAg                 >  8:317952/1‑149 (MQ=255)
                                                                                                                                   aTACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAg                 <  3:298811/149‑1 (MQ=255)
                                                                                                                                    tACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCCAAgg                >  5:113106/1‑149 (MQ=255)
                                                                                                                                       gACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGgcgc             >  7:164771/1‑149 (MQ=255)
                                                                                                                                        aCAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGgcgcg            >  8:77176/1‑149 (MQ=255)
                                                                                                                                               cAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCg     >  1:383222/1‑149 (MQ=255)
                                                                                                                                                  ggCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGc  <  6:307738/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCCGTTCAAGGCCGACCATGACAGCGCCCGCGCCACACTGGCCAAGGCC‑GAAGCCACCCGGTACCAGGCGCGCCTGCAAGAGCAGCGCTACCGCGAGCTGGTCGACGACAAAG‑CCGTCAGCCGCCAGGAATACGACAACGCCAAGGCCAGTTTCCTGCAGGCCGATGCCGAAGTGGCCGAAGCCAGGGCGGCGCTGGAGCGCGCCCGGCTGAACCTCGGCTATGCCACCGTGACCGCGCCGATCTCCGGCCGTATCGGCCGCGCCCAGGTCACCGAAGGCGCGCTGGTCGGGC  >  NC_002947/3915328‑3915620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑