Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 295,249 T→C 5.6% L218P (CTC→CCC)  ssuB → aliphatic sulfonates ABC transporter ATP‑binding protein SsuB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947295,2490TC 5.6% 319.4 / 6.2 107L218P (CTC→CCC) ssuBaliphatic sulfonates ABC transporter ATP‑binding protein SsuB
Reads supporting (aligned to +/- strand):  ref base T (56/45);  new base C (3/3);  total (59/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

Only 100 of 108 total aligned reads displayed.

TGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCAACTGCGC  >  NC_002947/295103‑295397
                                                                                                                                                  |                                                                                                                                                    
tGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCg                                                                                                                                                    <  1:238922/149‑1 (MQ=255)
tGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCCAGGTCGGGCTc                                                                                                                                                     >  3:221788/1‑148 (MQ=255)
 gCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGa                                                                                                                                                   >  4:186489/1‑149 (MQ=255)
   gTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGAcc                                                                                                                                                 >  6:185426/1‑149 (MQ=255)
   gTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGAcc                                                                                                                                                 <  4:128950/149‑1 (MQ=255)
        cccTTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGAGCCCGACCTcgcc                                                                                                                                            <  4:299151/149‑4 (MQ=255)
        cccGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGAGCCCGACCTcgcc                                                                                                                                            <  8:315032/149‑4 (MQ=255)
         ccGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTg                                                                                                                                           >  1:392877/1‑149 (MQ=255)
              tCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGa                                                                                                                                       <  7:42163/148‑1 (MQ=255)
              tCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGa                                                                                                                                       <  6:178178/148‑1 (MQ=255)
                 agaTGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTg                                                                                                                                   >  7:169516/1‑149 (MQ=255)
                    tGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCa                                                                                                                                >  2:202420/1‑149 (MQ=255)
                     gcagcaACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCAc                                                                                                                               >  2:344382/1‑149 (MQ=255)
                      cagcaACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACg                                                                                                                              <  8:41913/149‑1 (MQ=255)
                       agcaACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACgg                                                                                                                             >  5:72196/1‑149 (MQ=255)
                          aaCTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGccc                                                                                                                          >  1:48796/1‑149 (MQ=255)
                                  gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGcgcg                                                                                                                  >  5:50113/1‑149 (MQ=255)
                                  gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGcgcg                                                                                                                  >  6:253558/1‑149 (MQ=255)
                                  gAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGcgcg                                                                                                                  <  2:304043/149‑1 (MQ=255)
                                      gCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGt                                                                                                              >  4:219694/1‑149 (MQ=255)
                                      gCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGt                                                                                                              <  1:202420/149‑1 (MQ=255)
                                      gCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGt                                                                                                              >  3:203115/1‑149 (MQ=255)
                                        cTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTc                                                                                                            <  8:229725/149‑1 (MQ=255)
                                         tgtgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGt                                                                                                                 <  5:235541/143‑1 (MQ=255)
                                         tgtgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGt                                                                                                                 >  6:235541/1‑143 (MQ=255)
                                           tgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                         <  2:247350/149‑1 (MQ=255)
                                           tgGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                         <  6:72196/149‑1 (MQ=255)
                                             gCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCAcc                                                                                                       >  5:25395/1‑149 (MQ=255)
                                               gTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGt                                                                                                     <  5:253558/149‑1 (MQ=255)
                                                tCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTc                                                                                                    >  1:203155/1‑149 (MQ=255)
                                                   gCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTgg                                                                                                 <  6:364728/149‑1 (MQ=255)
                                                    cACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGc                                                                                                <  3:186489/149‑1 (MQ=255)
                                                    cACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGc                                                                                                >  4:103382/1‑149 (MQ=255)
                                                     aCGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCAcc                                                                                                       >  1:128105/1‑141 (MQ=255)
                                                     aCGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCAcc                                                                                                       <  2:128105/141‑1 (MQ=255)
                                                     aCGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGcc                                                                                               >  2:318276/1‑149 (MQ=255)
                                                       ggCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCcgc                                                                                             <  2:48796/149‑1 (MQ=255)
                                                       ggCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCcgc                                                                                             >  8:302618/1‑149 (MQ=255)
                                                          ttCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCt                                                                                          >  8:62416/1‑149 (MQ=255)
                                                            cACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACAGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTg                                                                                         >  3:163378/1‑148 (MQ=255)
                                                             aCTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTgg                                                                                        >  2:178786/1‑148 (MQ=255)
                                                             aCTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTgg                                                                                        <  6:50113/148‑1 (MQ=255)
                                                                 tACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGaaa                                                                                     <  1:318276/147‑1 (MQ=255)
                                                                 tACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGc                                                                                   >  4:115074/1‑149 (MQ=255)
                                                                 tACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGc                                                                                   >  6:379298/1‑149 (MQ=255)
                                                                   ctgctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCg                                                                                  <  5:185426/148‑1 (MQ=255)
                                                                    tgctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCg                                                                                  >  8:252897/1‑147 (MQ=255)
                                                                      ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                                <  2:203155/147‑1 (MQ=255)
                                                                      ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAg                                                                               <  8:329983/148‑1 (MQ=255)
                                                                      ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGt                                                                              >  6:344850/1‑149 (MQ=255)
                                                                      ctgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGt                                                                              >  1:413195/1‑149 (MQ=255)
                                                                       tgGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTg                                                                             >  6:442013/1‑149 (MQ=255)
                                                                         gTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                                <  3:242947/144‑1 (MQ=255)
                                                                         gTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                                >  4:242947/1‑144 (MQ=255)
                                                                           cACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTc                                                                          >  6:290242/1‑148 (MQ=255)
                                                                              ccACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAAcc                                                                      >  3:367191/1‑149 (MQ=255)
                                                                               cACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCg                                                                     <  4:203115/149‑1 (MQ=255)
                                                                                acgacgTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCgt                                                                    >  4:259519/1‑149 (MQ=255)
                                                                                      tCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTg                                                              <  4:163378/149‑1 (MQ=255)
                                                                                      tCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGGGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTg                                                              <  8:353437/149‑1 (MQ=255)
                                                                                           gAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAAcc                                                                      <  5:173771/136‑1 (MQ=255)
                                                                                           gAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCAc                                                         <  2:413195/149‑1 (MQ=255)
                                                                                           gAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCAc                                                         >  1:22379/1‑149 (MQ=255)
                                                                                                   tGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCCTGTTCTGTCCACCCCGGGCa                                                 >  2:195173/1‑149 (MQ=255)
                                                                                                    gCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCAc                                                >  7:196927/1‑149 (MQ=255)
                                                                                                      cGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTg                                              >  5:318551/1‑149 (MQ=255)
                                                                                                      cGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTg                                              >  4:269712/1‑149 (MQ=255)
                                                                                                       gTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTgc                                             >  3:197265/1‑149 (MQ=255)
                                                                                                        tGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTgcg                                            >  8:376871/1‑149 (MQ=255)
                                                                                                         ggCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTgcgc                                           >  1:377063/1‑149 (MQ=255)
                                                                                                            tGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGccc                                         >  7:5775/1‑148 (MQ=255)
                                                                                                             gACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGccc                                         >  4:148293/1‑147 (MQ=255)
                                                                                                              aCCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                         <  6:353797/82‑1 (MQ=255)
                                                                                                              aCCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCa                                                                                                         >  5:353797/1‑82 (MQ=255)
                                                                                                               ccGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGc                                     <  5:379298/149‑1 (MQ=255)
                                                                                                                cGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGcc                                    <  7:62416/149‑1 (MQ=255)
                                                                                                                    tGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGAt                                >  5:379487/1‑149 (MQ=255)
                                                                                                                      aTCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATcc                              >  4:14740/1‑149 (MQ=255)
                                                                                                                      aTCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATcc                              >  5:36133/1‑149 (MQ=255)
                                                                                                                      aTCCTGAGCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATc                               <  6:25395/148‑1 (MQ=255)
                                                                                                                        ccTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCCGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTg                            >  5:155739/1‑149 (MQ=255)
                                                                                                                         cTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTg                            >  7:54022/1‑148 (MQ=255)
                                                                                                                         cTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTg                            <  6:379487/148‑1 (MQ=255)
                                                                                                                           gATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                                >  2:79121/1‑94 (MQ=255)
                                                                                                                           gATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGaa                                                                                <  1:79121/94‑1 (MQ=255)
                                                                                                                               gAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGccc                      <  1:344382/148‑1 (MQ=255)
                                                                                                                                aGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAg                         >  7:376258/1‑144 (MQ=255)
                                                                                                                                aGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAg                         <  8:376258/144‑1 (MQ=255)
                                                                                                                                   aCGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTg                 <  4:197265/149‑1 (MQ=255)
                                                                                                                                    cagtgAGGTCGAGCCCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGc                >  8:106271/5‑149 (MQ=255)
                                                                                                                                     ggCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGcc               <  6:318551/149‑1 (MQ=255)
                                                                                                                                      gCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGcc                                    <  2:203782/127‑1 (MQ=255)
                                                                                                                                      gCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGcc                                    >  1:203782/1‑127 (MQ=255)
                                                                                                                                       tgAGGTCGAGCCCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGGCCa             >  6:51176/2‑149 (MQ=255)
                                                                                                                                          ggTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGc          <  7:376871/149‑1 (MQ=255)
                                                                                                                                           gTCGGGCTCGACCTGACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGc          <  3:219694/148‑1 (MQ=255)
                                                                                                                                           gTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCa         <  8:196927/149‑1 (MQ=255)
                                                                                                                                            tCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCaa        <  3:124644/149‑1 (MQ=255)
                                                                                                                                                gCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCAACTgc    >  8:342088/1‑149 (MQ=255)
                                                                                                                                                  tCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCAACTgcgc  >  2:179325/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGCGTTGACCCGTATCGAGATGCAGCAACTGATCGAACGCCTGTGGCGTCAGCACGGCTTCACTGTACTGCTGGTCACCCACGACGTCAGCGAGGCGGTTGCCGTGGCTGACCGGGTGATCCTGATCGAGGACGGCGAGGTCGGGCTCGACCTCACTGTTGACCTGGCACGGCCCCGGGCGCGTGGTTCGCACCGTCTGGCCGCGCTGGAAAGCGAAGTGCTCAACCGTGTTCTGTCCACCCCGGGCACTGCGCCCGAGCCGGATCCTGTAGCCCCTCTGCCCACGCAACTGCGC  >  NC_002947/295103‑295397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑