| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,984,043 | A→C | 8.5% | intergenic (+37/+153) | ilvE → / ← PP_3512 | branched‑chain‑amino acid aminotransferase/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,984,043 | 0 | A | C | 8.5% | 173.2 / 7.0 | 59 | intergenic (+37/+153) | ilvE/PP_3512 | branched‑chain‑amino acid aminotransferase/membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (31/23); new base C (2/3); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
CCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGG > NC_002947/3983895‑3984186 | ccACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCaa > 5:234405/1‑149 (MQ=255) aCGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATg < 4:276351/149‑1 (MQ=255) aCGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATg < 1:249968/149‑1 (MQ=255) gACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATgg > 5:407783/1‑148 (MQ=255) cTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATg > 7:22307/1‑149 (MQ=255) tGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGc > 1:416319/1‑149 (MQ=255) ggAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGcc > 2:226602/1‑149 (MQ=255) ttccaGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGc < 3:241243/143‑1 (MQ=255) gAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCATTGCTGGCATGcc < 1:412531/148‑1 (MQ=255) aaaaGGTCGGCCCGGTTACCCATAAGCTCTACAACGATCTGACCGGTATCCAGAGAGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCATTGCTGGCATGcccc < 4:258445/149‑1 (MQ=255) ggTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGttt < 4:60840/149‑1 (MQ=255) ggTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCAGACCGCTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGACTGAAACGCATCAGATGAATGAAAACGGGGCATGCCAGCAAAGGCATGCACCGttt > 4:90198/1‑149 (MQ=255) ggCCCGGTTAGCCAGAAGGTCTACAACGAGGTGTCGTGTTTGCCGAGCGGCGATGTAGAGGCACCGGCAGGCTGGAGCGTCTAGGTCGCGTGATACGCATCAGCTGTATGAAAACGGGGCATGCCAGCTATGGCATGCCCCGtttttt < 3:90198/148‑1 (MQ=255) cccGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGc > 8:238493/1‑132 (MQ=255) cccGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGc < 7:238493/132‑1 (MQ=255) cGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGGGTTGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGGATCAGCTGTATGAAAACGGGGAATGCCAGCAATGGCATGCCCCGTTTTTTCAtgt > 5:349721/1‑149 (MQ=255) cccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTac < 7:28961/149‑1 (MQ=255) ccAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTaca < 1:178119/149‑1 (MQ=255) gAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACgg > 6:34908/1‑149 (MQ=255) aCAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCg > 7:348158/1‑149 (MQ=255) cAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTGATGTGTTTACACGGCCCCCTCGt < 5:95985/149‑1 (MQ=255) aCGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAg < 2:325193/149‑1 (MQ=255) cGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCACCGTTTTTTCATGTG‑TTACACGGCCCCCTCGTAg > 4:397695/1‑147 (MQ=255) aGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAg > 5:3640/1‑149 (MQ=255) aGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAg < 2:416319/149‑1 (MQ=255) gCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGc < 8:22307/149‑1 (MQ=255) cGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGtt > 7:235915/1‑148 (MQ=255) gTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTc > 4:148264/1‑133 (MQ=255) gTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTc < 3:148264/133‑1 (MQ=255) gTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTAc > 6:306380/1‑149 (MQ=255) agagCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGa < 6:407783/149‑1 (MQ=255) gcgATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGc < 4:410682/149‑1 (MQ=255) gTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCacac > 1:149308/1‑149 (MQ=255) gCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCgc > 7:45999/1‑147 (MQ=255) cACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCgccag > 5:384644/1‑149 (MQ=255) cAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCaata < 6:234405/149‑1 (MQ=255) ggCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCaataat < 1:226602/149‑1 (MQ=255) gTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGga < 5:306380/148‑1 (MQ=255) aaGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTG‑TTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCa < 3:397695/149‑1 (MQ=255) gCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGtt > 8:209624/1‑149 (MQ=255) cTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGga < 4:236628/137‑1 (MQ=255) cTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGga > 3:236628/1‑137 (MQ=255) cTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACACGCGAATAGGACACACCCGCCAGCCAATAATGCCTGGGAGATAACAGTTg > 8:96627/1‑148 (MQ=255) aaaCGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAg < 6:3640/149‑1 (MQ=255) tATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAg > 1:255891/1‑149 (MQ=255) aTGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGc > 4:152046/1‑149 (MQ=255) aaCGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGc > 5:316308/1‑148 (MQ=255) aCGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGc > 5:255679/1‑147 (MQ=255) ggggCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCTAATAGGCCACACCCGCCCGCCAATAATGCCTGGGAGATCACCGTTGTCTGTGCAGCCGCATTTGCGGGCAAGc < 2:149308/149‑1 (MQ=255) gggCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGcc > 4:344585/1‑149 (MQ=255) ggCATGCCATTTCTGGCATGACCCGTTTTTTCATGTGTTTACACGGCCCCCTTGTTGGAGTGTGTTTACTCGCGAATAGGCTACACTCGCCAGCTAATAATGTCTGGGAGATCATTGTTGTTAGTGCa < 8:64955/128‑1 (MQ=255) ggCATGCCATTGCTGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGccc > 7:5000/1‑149 (MQ=255) ggCATGCCATTGCTGGCATGCCCCGTTTTTTAATGTGTTTACAAGGCCCCCTCGTAGGAGCGGGTTTACCAGAGAATAGGCCACACCCGCAAGAAAAAAATGCCTGGGAGAACACCGTTGTCAGTgaa > 7:64955/1‑126 (MQ=255) ggCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGccc > 4:394060/1‑149 (MQ=255) gCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCcg > 5:177984/1‑149 (MQ=255) ccAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCg > 7:55491/1‑149 (MQ=255) cAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGTCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTACAGCCGCATTAGCGGGCAAGCCCGCGCCgg < 8:235915/149‑1 (MQ=255) cAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCgg > 3:320511/1‑149 (MQ=255) cAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCg > 1:157713/1‑148 (MQ=255) cAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCATGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGTCAAGCCCGCGCCgg > 8:180872/1‑149 (MQ=255) | CCACGACCTGGAAAAGGTCGGCCCGGTTACCCAGAAGCTCTACAACGAGCTGACCGGTATCCAGAGCGGCGATGTAGAGGCACCGGCAGGCTGGATCGTCAAGGTCGCCTGAAACGCATCAGCTGTATGAAAACGGGGCATGCCAGCAATGGCATGCCCCGTTTTTTCATGTGTTTACACGGCCCCCTCGTAGGAGCGGGTTTACCCGCGAATAGGCCACACCCGCCAGCCAATAATGCCTGGGAGATCACCGTTGTCAGTGCAGCCGCATTAGCGGGCAAGCCCGCGCCGG > NC_002947/3983895‑3984186 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |