| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,995,334 | A→T | 8.2% | intergenic (‑99/+51) | PP_3521 ← / ← PP_3522 | membrane protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,995,334 | 0 | A | T | 8.2% | 167.5 / 3.6 | 58 | intergenic (‑99/+51) | PP_3521/PP_3522 | membrane protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (29/24); new base T (3/2); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GGCGCTGGTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTGCAGGTCG > NC_002947/3995191‑3995476 | ggCGCTGGTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCg < 8:194407/149‑1 (MQ=255)ggCGCTGGTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCg < 2:413308/149‑1 (MQ=255) gcgcTGGTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCgg < 1:411183/149‑1 (MQ=255) cgcTGGTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCGTAGCCGg‑t < 7:237487/149‑1 (MQ=255) gTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCGTAGCCGg‑tgcca < 3:298254/148‑4 (MQ=255) gCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCgg < 4:378843/147‑1 (MQ=255) ggTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGttt < 7:327834/149‑3 (MQ=255) gTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGAGGTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTa > 1:102108/1‑148 (MQ=255) tGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGAcc > 7:161380/1‑147 (MQ=255) cTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGcc < 8:16595/148‑1 (MQ=255) tCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCgg > 4:72339/1‑149 (MQ=255) gtgGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCtttt > 7:175747/1‑147 (MQ=255) gTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCa < 6:2405/146‑1 (MQ=255) gTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCa > 5:2405/1‑146 (MQ=255) gTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAgg < 3:72339/148‑1 (MQ=255) aCCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGtttt > 8:55917/1‑149 (MQ=255) aCCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGtttt < 8:207629/149‑1 (MQ=255) tctGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATTTACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAg > 8:138408/1‑149 (MQ=255) gTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGa > 3:95529/1‑149 (MQ=255) ggATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCAGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGAcgg > 6:384518/1‑149 (MQ=255) gttgttATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACggcggc > 5:290739/1‑148 (MQ=255) ttgttATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTa > 2:246566/1‑149 (MQ=255) tgttATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTAc < 7:406676/149‑1 (MQ=255) taGCGGCATTTAGTCAGGACCGGCACCGATTTACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCtt < 7:138408/149‑1 (MQ=255) taGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCt < 1:84871/148‑1 (MQ=255) cGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGa > 4:388947/1‑149 (MQ=255) ggCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGAt < 5:321526/149‑1 (MQ=255) aGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGATCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTccac < 1:52940/148‑1 (MQ=255) gTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTccac > 2:391882/1‑147 (MQ=255) tCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACgg > 5:110925/1‑87 (MQ=255) tCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACgg < 6:110925/87‑1 (MQ=255) tCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCtt < 3:218701/136‑1 (MQ=255) tCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCtt > 4:218701/1‑136 (MQ=255) aGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGc < 8:175747/148‑1 (MQ=255) ggACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCCAGCCGGCTTTTCAGGTTTTGAGCAAGCAGGCGGCTACTGCTTCGCTGTCCAGCAGCAt > 8:400795/1‑149 (MQ=38) gACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATg > 5:64347/1‑149 (MQ=255) aCCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGc < 4:95529/149‑1 (MQ=255) ccGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAg < 8:161380/149‑1 (MQ=255) cAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGTCGTCCAGTGCCAGcc > 6:302916/1‑149 (MQ=255) cAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCAACTGCTTAGATCTCCACCAGCATGCCGTCCAGTGCCAGcc > 1:280720/1‑149 (MQ=255) cAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGACGG‑TGTTCGCGGGTAAACCCGCGCACACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCACCACAAGCATGCCGTCCAGTGCCAGcc > 5:124225/1‑149 (MQ=255) aGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCACACGGGCGACCGCGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCGACTGCGTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCgg > 2:369086/1‑149 (MQ=255) gaagaaCCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCgggg > 1:369780/1‑149 (MQ=255) agaaCCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCa > 8:47464/1‑149 (MQ=255) ccGTGAGGATGGCACCGGCTACGCCGGTTGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTa > 1:247875/1‑149 (MQ=255) aGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCg > 1:127305/1‑149 (MQ=255) ggATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCg > 4:301030/1‑117 (MQ=255) ggATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCg < 3:301030/117‑1 (MQ=255) cgcgaacACCGGCGTAGCCGG‑TGCTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGt > 7:292146/7‑149 (MQ=255) cgcgaacACCGGCGTAGCCGG‑TGCTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGt > 7:286287/7‑149 (MQ=255) aTGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGTCGGGGCACCGGTATCAGCGTGc < 1:391882/149‑1 (MQ=255) gaacACCGGCGTAGCCGG‑TGCTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTg > 4:321687/4‑147 (MQ=255) gCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTGCAg < 2:127305/148‑1 (MQ=255) cACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTGCAGGt > 4:231224/1‑149 (MQ=255) aCCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATc < 8:204314/137‑1 (MQ=255) aCCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATc > 7:204314/1‑137 (MQ=255) aCCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTGCAGGTc > 6:316427/1‑149 (MQ=255) ccGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTGCAGGTCg < 2:102108/149‑1 (MQ=255) | GGCGCTGGTCTTGCGGTCAGTAAGCGTGGTCGTGCCTGTACTCATGGTGTGGTCACCTCTGA‑GTGGGATTGTTGTTATAGCGGCATTTAGTCAGGACCGGCACCGATATACAAGGAAGAACCACCGTGAGGATGGCACCGGCTACGCCGG‑TGTTCGCGGGTAAACCCGCGCCCACGGGCGACCGAGCCGGCTTTTCAGGTTTTGAGCAAGACGGCGGCTACTGCTTCGATCTCCACCAGCATGCCGTCCAGTGCCAGCCGGGGCACCGGTATCAGCGTGCAGGTCG > NC_002947/3995191‑3995476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |