Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,147,929 G→T 7.8% G671V (GGC→GTC)  PP_3651 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,147,9290GT 7.8% 161.6 / 3.9 51G671V (GGC→GTC) PP_3651two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (20/27);  new base T (2/2);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GGGCAGTTTCACAGCTCAGCTCCTCGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGCCAAACCCTACTCGGCAGAACAGGTGTGCCAG  >  NC_002947/4147783‑4148074
                                                                                                                                                    |                                                                                                                                                 
gggCAGTTTCACAGCTCAGCTGCTCGAGGACCACGGCATACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGc                                                                                                                                                  >  1:77812/1‑149 (MQ=255)
 ggCAGTTTCACAGCTCAGCTCCTCGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCtt                                                                                                                                                <  2:67241/149‑1 (MQ=255)
 ggCAGTTTCACAGCTCAGCTCCTCGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCtt                                                                                                                                                <  8:218972/149‑1 (MQ=255)
  gCAGTTTCACAGCTCAGCTCCTCGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTg                                                                                                                                               <  5:296912/149‑1 (MQ=255)
      tttCACAGCTCAGCTCCTCGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCAc                                                                                                                                           >  4:16844/1‑149 (MQ=255)
                      ctcGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTcg                                                                                                                           >  3:360643/1‑149 (MQ=255)
                       tcGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTcgc                                                                                                                          >  6:160059/1‑149 (MQ=255)
                         gAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTcgccg                                                                                                                        >  4:322198/1‑149 (MQ=255)
                          aGGACCACTGC‑TACCAAATCAGCTATGCACGGTAGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCAGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTcgccgc                                                                                                                       <  4:218135/149‑1 (MQ=255)
                          aGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTcgccgc                                                                                                                       >  7:116736/1‑149 (MQ=255)
                              ccACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAgc                                                                                                                   <  8:151159/149‑1 (MQ=255)
                               cACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTgg                                                                                                                                                   <  6:113512/116‑1 (MQ=255)
                               cACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTgg                                                                                                                                                   >  5:113512/1‑116 (MQ=255)
                                 cGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCAGGTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAgcgc                                                                                                                 >  1:102842/1‑149 (MQ=255)
                                  ggC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGATTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGccc                                                                                                               >  3:390593/1‑149 (MQ=255)
                                    c‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGc                                                                                                             <  8:18501/149‑1 (MQ=255)
                                          aaaTCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTg                                                                                                        >  3:332072/1‑149 (MQ=255)
                                           aaTCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTGTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGc                                                                                                       <  6:145343/149‑1 (MQ=255)
                                              cAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGTCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCgg                                                                                                    >  7:46342/1‑149 (MQ=255)
                                              cAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGTCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCgg                                                                                                    >  7:53034/1‑149 (MQ=255)
                                               aGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGt                                                                                                   <  3:143492/149‑1 (MQ=255)
                                                  tATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAgc                                                                                                                   <  2:425375/130‑1 (MQ=255)
                                                  tATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAgc                                                                                                                   >  1:425375/1‑130 (MQ=255)
                                                       aCGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGa                                                                                           >  3:346341/1‑149 (MQ=255)
                                                         ggTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACt                                                                                         >  4:362594/1‑149 (MQ=255)
                                                         ggTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACt                                                                                         <  8:116736/149‑1 (MQ=255)
                                                           tCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTc                                                                                       >  8:329005/1‑149 (MQ=255)
                                                             ggCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCgg                                                                                     <  8:241398/149‑1 (MQ=255)
                                                             ggCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCgg                                                                                     <  4:325343/149‑1 (MQ=255)
                                                              gCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCggg                                                                                    <  8:395351/149‑1 (MQ=255)
                                                              gCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCggg                                                                                    <  3:362594/149‑1 (MQ=255)
                                                              gCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCggg                                                                                    >  6:231050/1‑149 (MQ=255)
                                                                       gcgcTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGa                                                                           <  4:346341/149‑1 (MQ=255)
                                                                             gcgcA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGAt                                                                     <  1:232994/149‑1 (MQ=255)
                                                                               gcA‑GTTGACTGGCCCGGCAGGTGTTTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTg                                                                   <  3:168380/149‑1 (MQ=255)
                                                                                cA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGc                                                                  <  2:102842/149‑1 (MQ=255)
                                                                                          ggCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCg                                                          <  4:390593/148‑1 (MQ=255)
                                                                                           gCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGTCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCgg                                                         <  8:46342/148‑1 (MQ=255)
                                                                                           gCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGTCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCgg                                                         <  8:53034/148‑1 (MQ=255)
                                                                                                         tttGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCAc                                          >  6:156475/1‑149 (MQ=255)
                                                                                                         tttGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCAc                                          >  3:119239/1‑149 (MQ=255)
                                                                                                          ttGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCAc                                          <  5:156475/148‑1 (MQ=255)
                                                                                                              tGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACGAAGGGTTCACCTTcc                                     >  2:341809/1‑149 (MQ=255)
                                                                                                              tGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTcc                                     <  5:160059/149‑1 (MQ=255)
                                                                                                                cAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTg                                   <  6:170637/149‑1 (MQ=255)
                                                                                                                cAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTg                                   <  3:322198/149‑1 (MQ=255)
                                                                                                                 aGTGTTATCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTgg                                  <  2:383939/149‑1 (MQ=255)
                                                                                                                       ttCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCCCCAAGGGTTCACCTTCCTGGCCaaa                             <  4:332072/148‑1 (MQ=255)
                                                                                                                               tgGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGCCAAACCCTACTc                     <  2:77812/148‑1 (MQ=255)
                                                                                                                                                ggTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGCCAAACCCTACTCGGCAGAACAGGTGTGc     >  5:2026/1‑147 (MQ=255)
                                                                                                                                                ggTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGCCAAACCCTACTCGGCAGAACAGGTGTGc     >  5:137899/1‑147 (MQ=255)
                                                                                                                                                ggTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCACTTGACGGTGATCCTGACTTCGGGCTAGAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGACAAACCATACTCGGCAGAAAAGGTTTGc     >  8:214795/1‑147 (MQ=255)
                                                                                                                                                 gTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGCCAAACCCTACTCGGCAGAACAGGTGTGCCAg  <  7:329005/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGGCAGTTTCACAGCTCAGCTCCTCGAGGACCACGGC‑TACCAAATCAGCTATGCACGGTCGGCAGAAGAAGCGCTGGCGCA‑GTTGACTGGCCCGGCAGGTGATTTTGATGCAGTGTTTTCCGATGTGGTCATGCCCGGCATGGGTGGCTTGGCACTGGCCCGCGAACTTCGCCGCCAGCGCCCGCAGTTGCCGGTGATCCTGACTTCGGGCTATAGCGAGGCGATTGCCGAAGGCGGGCACCAAGGGTTCACCTTCCTGGCCAAACCCTACTCGGCAGAACAGGTGTGCCAG  >  NC_002947/4147783‑4148074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑