Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,260,591 A→G 5.8% V724A (GTG→GCG)  PP_3734 ← ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,260,5910AG 5.8% 193.9 / 2.1 70V724A (GTG→GCG) PP_3734ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (33/31);  new base G (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

CGGTAAACGCCAGCCGAAGGCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGA  >  NC_002947/4260443‑4260739
                                                                                                                                                    |                                                                                                                                                     
cGGTAAACGCCAGCCGAAGGCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCa                                                                                                                                                       >  7:212125/1‑149 (MQ=255)
  gTAAACGCCAGCCGAAGGCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCAcc                                                                                                                                                     >  5:239015/1‑149 (MQ=255)
        gCCAGCCGAAGGCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGt                                                                                                                                               >  2:312361/1‑149 (MQ=255)
              cGAAGGCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCGGTTGCATGCGCCGCACCCCCAGTGCCca                                                                                                                                          >  5:386549/1‑148 (MQ=255)
                  ggCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑g                                                                                                                                    >  3:417259/1‑149 (MQ=255)
                    ccTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGt                                                                                                                                  <  5:283393/149‑1 (MQ=255)
                    ccTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGt                                                                                                                                  <  6:386549/149‑1 (MQ=255)
                    ccTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGt                                                                                                                                  >  3:122603/1‑149 (MQ=255)
                        cACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGcc                                                                                                                               >  7:56548/1‑148 (MQ=255)
                        cACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGAGGAATCGCCAGCAGTACGGTGAATCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAAACAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGcc                                                                                                                               >  5:433894/1‑148 (MQ=255)
                              caccacTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGc                                                                                                                        >  1:245204/1‑149 (MQ=255)
                               accacTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGc                                                                                                                        <  8:252625/148‑1 (MQ=255)
                               accacTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGc                                                                                                                        <  4:378659/148‑1 (MQ=255)
                                ccacTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGcc                                                                                                                       >  6:103521/1‑148 (MQ=255)
                                    tGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGcc                                                                                                                  >  7:105694/1‑149 (MQ=255)
                                        accaggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCgcgc                                                                                                              <  4:417259/149‑1 (MQ=255)
                                        accaggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCgcgc                                                                                                              <  1:150211/149‑1 (MQ=255)
                                          caggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACGAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCt                                                                                                            <  1:124422/149‑1 (MQ=255)
                                           aggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTg                                                                                                           >  7:175648/1‑149 (MQ=255)
                                            ggcaccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTgg                                                                                                          <  6:310904/149‑1 (MQ=255)
                                                ccaggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCa                                                                                                      <  8:175648/149‑1 (MQ=255)
                                                 caggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAg                                                                                                     >  8:390316/1‑149 (MQ=255)
                                                  aggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAgg                                                                                                    <  4:428277/149‑1 (MQ=255)
                                                  aggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAgg                                                                                                    <  8:212125/149‑1 (MQ=255)
                                                  aggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAgg                                                                                                    >  5:120015/1‑149 (MQ=255)
                                                  aggcCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGAGCCAACTGGCCCCGCCGCGCCTGGCCCAgg                                                                                                    >  4:138808/1‑149 (MQ=255)
                                                      cAGCAACAGCCCAAGCGGAAACGCCAGCATAACGGTTAAGCCGCTGAGCATTAGGGGCTGCCCCAGCCTCACCCAGACCAGTTCCAGGCGCGGCCCCCCAATTGCCACCACG‑GGTGCCAACAAGCCCAGCGGCGCGTGGCCCAggggct                                                                                                >  8:144889/1‑146 (MQ=255)
                                                           aCAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAgg                                                                                           <  2:245204/149‑1 (MQ=255)
                                                            cAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGt                                                                                          <  1:312361/149‑1 (MQ=255)
                                                             aGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGtt                                                                                         <  1:172673/149‑1 (MQ=255)
                                                                ccAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTtgat                                                                                      >  2:155390/1‑149 (MQ=255)
                                                                 cAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑TGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTtgatg                                                                                     <  8:105694/149‑1 (MQ=255)
                                                                  aaGCTGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTtgatga                                                                                    <  5:122863/149‑1 (MQ=255)
                                                                   aggggAATCGCCAGCAGTTCGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCATACCAGTTGCATGCGCCGCACGCGCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGaa                                                                                   <  3:138808/146‑1 (MQ=255)
                                                                            gCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTAGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCca                                                                           <  7:144889/148‑1 (MQ=255)
                                                                             ccAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCcaca                                                                         >  2:255080/1‑149 (MQ=255)
                                                                                gcagTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCg                                                                      >  4:173567/1‑149 (MQ=255)
                                                                                 cagTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCgg                                                                     >  8:262091/1‑149 (MQ=255)
                                                                                  agTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGc                                                                    <  6:239015/149‑1 (MQ=255)
                                                                                           aaGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCCCCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGCTGAACCACGCGACACCGGCCCCGCCAAg                                                           >  5:246843/1‑149 (MQ=255)
                                                                                            aGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAgg                                                          <  5:103521/149‑1 (MQ=255)
                                                                                             gCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCGCCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAggg                                                         >  7:55628/1‑149 (MQ=255)
                                                                                             gCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCGCCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAggg                                                         >  7:48788/1‑149 (MQ=255)
                                                                                                gCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGAGGAACAATGCCACACCGGCCACGCCAAGGGt                                                        >  5:101411/1‑147 (MQ=255)
                                                                                                 cTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAg                                                     >  1:233550/1‑149 (MQ=255)
                                                                                                       aTGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCCGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGtt                                               <  6:433894/149‑1 (MQ=255)
                                                                                                       aTGACTCTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGtt                                               <  4:11052/149‑1 (MQ=255)
                                                                                                         gAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGtt                                               >  2:282546/1‑147 (MQ=255)
                                                                                                          aGTGTCTGCACCGGGCTCAACCAGACCAGTTGGATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGGAGGTTGATGAACAACGCCAGACCGTCCACGCCAAGGGTAAGGCTGTTgag                                            <  5:341270/149‑1 (MQ=255)
                                                                                                           gtgtCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTgaga                                           <  6:120015/149‑1 (MQ=255)
                                                                                                           gtgtCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTgaga                                           <  3:215901/149‑1 (MQ=255)
                                                                                                              tCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCt                                        >  6:234757/1‑149 (MQ=255)
                                                                                                                  ccccAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTg                                       <  5:16299/146‑1 (MQ=255)
                                                                                                                      aGGCTCAACCAGACCAGTTGCATGCGCCGCGCCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggc                                <  8:55628/149‑1 (MQ=255)
                                                                                                                      aGGCTCAACCAGACCAGTTGCATGCGCCGCGCCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggc                                <  8:48788/149‑1 (MQ=255)
                                                                                                                        gCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGACCAAGCCGCGCCTGGCCCAGGGTCAGCAGCTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGATGTTga                                             >  7:79011/1‑134 (MQ=255)
                                                                                                                          tCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggcggc                             >  1:188781/1‑148 (MQ=255)
                                                                                                                           cAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAgg                                                          >  6:107462/1‑118 (MQ=255)
                                                                                                                           cAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAgg                                                          <  5:107462/118‑1 (MQ=255)
                                                                                                                             accagaccagTTGCATGCGCCGGACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAg                         >  7:272094/1‑149 (MQ=255)
                                                                                                                             accagaccagTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAg                         >  6:243064/1‑149 (MQ=255)
                                                                                                                             accagaccagTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAg                         >  8:19129/1‑149 (MQ=255)
                                                                                                                              ccagaccagTTGAATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCAGGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggcg                               >  5:375410/1‑142 (MQ=255)
                                                                                                                              ccagaccagTTGAATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTggcg                               <  6:375410/142‑1 (MQ=255)
                                                                                                                               cagaccagTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGt                       >  8:92544/1‑149 (MQ=255)
                                                                                                                                   ccagTTGCATGCGCCGGACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCgg                   <  8:272094/149‑1 (MQ=255)
                                                                                                                                   ccagTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCgg                   >  3:82440/1‑149 (MQ=255)
                                                                                                                                            tGCGCCGCACCCCCAGTGCCCACAGCGGGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGAc           >  1:226183/1‑149 (MQ=255)
                                                                                                                                                 cgcACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGTGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACgtcggt     <  7:390316/149‑1 (MQ=255)
                                                                                                                                                 cgcACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCACGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACgtcggt     >  1:205337/1‑149 (MQ=255)
                                                                                                                                                  gcACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACgtcggtc    <  5:243064/149‑1 (MQ=255)
                                                                                                                                                    aCCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGa  >  5:16829/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGTAAACGCCAGCCGAAGGCCTGCACGTTCACCACTGCGACCAGGCACCAGGCCAGCAACAGCCCAAGCGGAATCGCCAGCAGTACGGTGAAGCCGCTGAGCATGAGTGTCTGCCCCAGGCTCAACCAGACCAGTTGCATGCGCCGCACCCCCAGTGCCCACAGC‑GGTGCCAACTGGCCCAGCCGCGCCTGGCCCAGGGTCAGCAGGTTGATGAACAACGCCACACCGGCCACGCCAAGGGTAAGGCTGTTGAGAGCTGCGGTGGCGGCAAAGGTGCGGTTGAAGACGTCGGTCGA  >  NC_002947/4260443‑4260739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑