Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,283,412 A→G 5.5% E5E (GAA→GAG)  bktB → beta‑ketothiolase BktB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,283,4120AG 5.5% 215.1 / 2.2 73E5E (GAA→GAG) bktBbeta‑ketothiolase BktB
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base G (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCCAGGTTGTTTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGCAAGTCGGCCACA  >  NC_002947/4283266‑4283554
                                                                                                                                                   |                                                                                                                                              
gCCAGGTTGTTTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGaaa                                                                                                                                               >  5:396343/1‑148 (MQ=255)
 ccAGGTTGTTTGAACAGATTCGGCATAGGCTGCAACCGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATc                                                                                                                                             <  7:381943/149‑1 (MQ=255)
    ggTTGTTTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTAc                                                                                                                                          <  3:393903/149‑1 (MQ=255)
    ggTTGTTTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTAc                                                                                                                                          >  2:77969/1‑149 (MQ=255)
     gttgttTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTAcg                                                                                                                                         <  6:396343/149‑1 (MQ=255)
     gttgttTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTAGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTAcg                                                                                                                                         >  5:210124/1‑149 (MQ=255)
      ttttttGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGAATCGATCCCACTTCGGGGGCTCCAACCATTCTCTCAGAGCCTGGCCCTGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTAcgt                                                                                                                                        <  7:343896/146‑1 (MQ=255)
        gttTGAACAGATTCGGCATAGGCTGCAACAGAGCCCTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTAcgtc                                                                                                                                       <  4:204807/148‑1 (MQ=255)
             aaCAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGt                                                                                                                                 >  2:326606/1‑149 (MQ=255)
                  aTTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGccgt                                                                                                                            >  1:352669/1‑149 (MQ=255)
                   ttCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGTGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCTCAGAAATCTACGTCGTCAGTGccgt                                                                                                                            <  4:248189/148‑1 (MQ=255)
                    tCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGccgtcc                                                                                                                          >  7:158841/1‑149 (MQ=255)
                    tCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGccgtcc                                                                                                                          >  2:338586/1‑149 (MQ=255)
                      ggCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGccgtccgt                                                                                                                        <  3:109143/149‑1 (MQ=255)
                       gCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGccgtccgt                                                                                                                        >  2:43549/1‑148 (MQ=255)
                       gCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGtt                                                                                                                       >  8:189787/1‑149 (MQ=255)
                        cATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTc                                                                                                                      <  1:47280/149‑1 (MQ=255)
                         aTAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCTCTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCa                                                                                                                     <  8:163478/149‑1 (MQ=255)
                             gCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCa                                                                                                                 >  4:26037/1‑149 (MQ=255)
                                 cAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCAtt                                                                                                               <  8:202155/147‑1 (MQ=255)
                                 cAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCAtt                                                                                                               <  4:381835/147‑1 (MQ=255)
                                    cAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATtggtgg                                                                                                          <  1:80025/149‑1 (MQ=255)
                                        gCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCttt                                                                                                      <  6:210124/149‑1 (MQ=255)
                                         ccTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGGAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAGCCAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCttt                                                                                                      <  1:326606/149‑1 (MQ=255)
                                          cTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTgg                                                                                                    <  1:77969/149‑1 (MQ=255)
                                          cTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTgg                                                                                                    >  6:240938/1‑149 (MQ=255)
                                             tGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCg                                                                                                  <  2:352669/148‑1 (MQ=255)
                                                   gACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTcc                                                                                             <  3:193548/147‑1 (MQ=255)
                                                   gACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCt                                                                                           >  3:407644/1‑149 (MQ=255)
                                                    aCTCGATCCCACTTCGGGGCCGCCAACCATTCACGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCt                                                                                           >  3:122501/1‑148 (MQ=255)
                                                        gATCCCACTTCGGGGCCGCCAACCATTCACGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCaa                                                                                        <  4:122501/147‑1 (MQ=255)
                                                          tCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGAc                                                                                    <  4:407644/149‑1 (MQ=255)
                                                             cACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTg                                                                                 >  7:126144/1‑149 (MQ=255)
                                                              aCTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGc                                                                                <  8:158841/149‑1 (MQ=255)
                                                              aCTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGc                                                                                >  5:437565/1‑149 (MQ=255)
                                                                    gggCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTgg                                                                          >  5:55882/1‑149 (MQ=255)
                                                                     ggCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTcc                                                                                             <  7:226998/129‑1 (MQ=255)
                                                                     ggCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTcc                                                                                             >  8:226998/1‑129 (MQ=255)
                                                                     ggCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGc                                                                         <  1:338586/149‑1 (MQ=255)
                                                                       ccgccAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGcc                                                                        >  3:304477/1‑148 (MQ=255)
                                                                        cgccAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGa                                                                      >  2:255329/1‑149 (MQ=255)
                                                                          ccAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGccaggt                                                                    >  1:66764/1‑145 (MQ=255)
                                                                           cAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCt                                                                   <  6:55882/149‑1 (MQ=255)
                                                                            aaCCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTg                                                                  >  8:374526/1‑149 (MQ=255)
                                                                            aaCCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTg                                                                  >  7:304155/1‑149 (MQ=255)
                                                                              ccATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGc                                                                             <  5:331749/136‑1 (MQ=255)
                                                                              ccATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGc                                                                             >  6:331749/1‑136 (MQ=255)
                                                                               cATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAGATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGcc                                                               >  8:20755/1‑149 (MQ=255)
                                                                               cATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAGATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGc                                                                >  8:14378/1‑148 (MQ=255)
                                                                                aTTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCa                                                              >  4:334949/1‑149 (MQ=255)
                                                                                 ttCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAg                                                             >  4:3520/1‑149 (MQ=255)
                                                                                    tcGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAgcgc                                                          >  1:51081/1‑149 (MQ=255)
                                                                                       cAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGt                                                       <  8:304155/149‑1 (MQ=255)
                                                                                       cAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGt                                                       <  2:51081/149‑1 (MQ=255)
                                                                                          agCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGAc                                                    <  3:88844/149‑1 (MQ=255)
                                                                                                 cccGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAGATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGcgcc                                             <  7:14378/149‑1 (MQ=255)
                                                                                                 cccGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAGATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGcgcc                                             <  7:20755/149‑1 (MQ=255)
                                                                                                           ccACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGAGGTTCCCTCAAGGACCTGCCGCTGGCCCACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGc                                   >  1:400498/1‑149 (MQ=255)
                                                                                                                  ttCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCggg                            >  3:79088/1‑149 (MQ=255)
                                                                                                                       aaTCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTgg                       >  6:169699/1‑149 (MQ=255)
                                                                                                                       aaTCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTgg                       >  2:327588/1‑149 (MQ=255)
                                                                                                                       aaTCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTg                        >  4:115194/1‑148 (MQ=255)
                                                                                                                       aaTCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTg                        <  3:115194/148‑1 (MQ=255)
                                                                                                                        aTCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGgc                      <  3:26037/149‑1 (MQ=255)
                                                                                                                            gAGCCCTTATGAG‑CAGCGCAGAAATTTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGgccgcc                  >  8:13687/1‑149 (MQ=255)
                                                                                                                            gAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGgccgcc                  <  1:327588/149‑1 (MQ=255)
                                                                                                                            gAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGgccgcc                  <  5:169699/149‑1 (MQ=255)
                                                                                                                            gAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGgccgcc                  >  5:123527/1‑149 (MQ=255)
                                                                                                                                 cTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGc              >  4:383762/1‑148 (MQ=255)
                                                                                                                                    aTGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGCaa            <  3:334949/147‑1 (MQ=255)
                                                                                                                                    aTGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGCAAGt          >  8:249479/1‑149 (MQ=255)
                                                                                                                                       ag‑cagcGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCTGCCGAGCAAGTCg        <  6:437565/148‑1 (MQ=255)
                                                                                                                                             cgcAGAAATTTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGCAAGTCGGCcaca  <  7:13687/149‑1 (MQ=255)
                                                                                                                                             cgcAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGCAAGTCGGCcaca  >  5:57370/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GCCAGGTTGTTTGAACAGATTCGGCATAGGCTGCAACAGAGCCTTTGCCTAGACTCGATCCCACTTCGGGGCCGCCAACCATTCTCGCAGAGCCTGGCCCGGTCCCGCCACGCCTTCGCAATCGGAGCCCTTATGAG‑CAGCGCAGAAATCTACGTCGTCAGTGCCGTCCGTTCAGCCATTGGTGGCTTTGGCGGTTCCCTCAAGGACCTGCCGCTGGCCGACCTGGCCAGCGCCGTGACCCGCGCCGCCATCGAGCGTTCGGGCCTGGCCGCCGAGCAAGTCGGCCACA  >  NC_002947/4283266‑4283554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑