Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,331,666 T→A 5.6% L122L (CTT→CTA) ‡ PP_3803 → cation ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,331,6660TA 5.6% 272.4 / 4.6 89L122L (CTT→CTA) ‡PP_3803cation ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (43/40);  new base A (2/3);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

TCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTGGCGCTGCCGC  >  NC_002947/4331518‑4331814
                                                                                                                                                    |                                                                                                                                                    
tCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCtt                                                                                                                                                      <  3:52592/149‑1 (MQ=255)
     ctgGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGgcgc                                                                                                                                                 >  1:293993/1‑149 (MQ=255)
       gggTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGgcgc                                                                                                                                                 >  2:328507/1‑147 (MQ=255)
        ggTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGcc                                                                                                                                                <  2:210925/147‑1 (MQ=255)
        ggTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAg                                                                                                                                              >  8:77112/1‑149 (MQ=255)
         gTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGc                                                                                                                                             >  2:426875/1‑149 (MQ=255)
         gTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCCAAGCGCCAGc                                                                                                                                             <  8:134407/149‑1 (MQ=255)
          tGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGcc                                                                                                                                            >  2:127934/1‑149 (MQ=255)
            aGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCCAAGCGCCAGcgac                                                                                                                                          <  2:103305/149‑4 (MQ=255)
              cccTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCctgct                                                                                                                                        >  5:44696/1‑149 (MQ=255)
                cTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCctgctgc                                                                                                                                      >  1:415260/1‑149 (MQ=255)
                   taCCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCctgctgctg                                                                                                                                    <  8:169116/148‑1 (MQ=255)
                   taCCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCctgctgct                                                                                                                                     <  7:46102/147‑1 (MQ=255)
                    aCCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGAt                                                                                                                                  >  5:109304/1‑149 (MQ=255)
                     ccGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGTCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATg                                                                                                                                 >  6:162300/1‑149 (MQ=255)
                      cGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGc                                                                                                                                >  7:251005/1‑149 (MQ=255)
                        gCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTg                                                                                                                              >  3:227908/1‑149 (MQ=255)
                          ccTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGTATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTggc                                                                                                                            <  6:70696/149‑1 (MQ=255)
                          ccTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTgg                                                                                                                             >  4:235634/1‑148 (MQ=255)
                           cTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTggca                                                                                                                           <  8:299084/149‑1 (MQ=255)
                              aCGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTggcagg                                                                                                                         >  6:52732/1‑148 (MQ=255)
                               cGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTggcaggc                                                                                                                        <  1:275505/148‑1 (MQ=255)
                                ggCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCaa                                                                                                                      <  7:11140/149‑1 (MQ=255)
                                 gCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAg                                                                                                                     <  2:415260/149‑1 (MQ=255)
                                    gCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATc                                                                                                                  <  5:52732/149‑1 (MQ=255)
                                         ttCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACg                                                                                                              <  7:289974/148‑1 (MQ=255)
                                                aCTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACAt                                                                                                       >  3:286876/1‑148 (MQ=255)
                                                 ctgcctgcTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATc                                                                                                      <  1:127934/148‑1 (MQ=255)
                                                   gcggggggTTCGGCCTCCGGGTCCAGTTCCTGCGCACCCTTACGGGGCGGTCGCGGTTTACCCTGTTTGGCGTGTTCCTGTGGGGGTCGCTGGCGCTGGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATc                                                                                                      <  8:63200/139‑1 (MQ=255)
                                                    cctgcTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTc                                                                                                   >  2:404601/1‑148 (MQ=255)
                                                      tgctgTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTc                                                                                                   >  2:207704/1‑146 (MQ=255)
                                                      tgctgTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTAGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTc                                                                                                   >  5:131988/1‑146 (MQ=255)
                                                        ctgTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCgag                                                                                                <  1:328507/147‑1 (MQ=255)
                                                         tgTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCG‑CGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACg                                                                                            >  3:349291/1‑149 (MQ=255)
                                                             cGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGc                                                                                         >  7:345152/1‑149 (MQ=255)
                                                              ggCATCCTGCTCAACTTCCTGCTCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGc                                                                                         <  6:263672/148‑1 (MQ=255)
                                                              ggCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGc                                                                                         <  3:196532/148‑1 (MQ=255)
                                                                 aTCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTgtt                                                                                     <  3:235634/149‑1 (MQ=255)
                                                                    cTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTgttcg                                                                                   <  1:404601/148‑1 (MQ=255)
                                                                     tGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCGGGCGCTTGGCGCCAGCCTGCTGCTGCTGCTGGCAGGCAAGATCAACGTGCACAGCATCGAGAGCGTGCTgttcggt                                                                                 >  4:35736/1‑149 (MQ=255)
                                                                        tCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGca                                                                                                          >  5:180910/1‑121 (MQ=255)
                                                                        tCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGca                                                                                                          <  6:180910/121‑1 (MQ=255)
                                                                          aaCTTCCTGCGCAACCGTACCGGGCTGTCG‑CGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTg                                                                           <  4:349291/149‑1 (MQ=255)
                                                                           aCTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTg                                                                           <  4:286876/149‑1 (MQ=255)
                                                                             ttCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCt                                                                         >  3:180142/1‑149 (MQ=255)
                                                                              tCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCt                                                                         >  4:61109/1‑148 (MQ=255)
                                                                               ccTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCt                                                                         <  8:251005/147‑1 (MQ=255)
                                                                               ccTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGa                                                                       >  3:382239/1‑149 (MQ=255)
                                                                                 tGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTgtt                                                                                     >  7:88677/1‑133 (MQ=255)
                                                                                 tGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTgtt                                                                                     <  8:88677/133‑1 (MQ=255)
                                                                                 tGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGAcc                                                                     >  1:235901/1‑149 (MQ=255)
                                                                                  gcgcAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCg                                                                    >  8:167784/1‑149 (MQ=255)
                                                                                   cgcAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGAcc                                                                     <  1:207704/147‑1 (MQ=255)
                                                                                   cgcAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGCCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGAcc                                                                     <  4:372161/147‑1 (MQ=255)
                                                                                           tACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGccc                                                           <  6:109304/149‑1 (MQ=255)
                                                                                           tACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGcc                                                            >  8:18922/1‑148 (MQ=255)
                                                                                            aCCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGCGAACGTGCTgttc                                                                                    >  7:3578/1‑123 (MQ=255)
                                                                                            aCCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTgttc                                                                                    <  8:3578/123‑1 (MQ=255)
                                                                                              cGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAg                                                         >  5:63739/1‑148 (MQ=255)
                                                                                                ggCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGAc                                                      >  4:179730/1‑149 (MQ=255)
                                                                                                     tCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGc                                                  <  7:77112/148‑1 (MQ=255)
                                                                                                     tCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCt                                                 <  2:293993/149‑1 (MQ=255)
                                                                                                     tCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTGCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGc                                                  <  5:249613/148‑1 (MQ=255)
                                                                                                     tCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTAGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGTAccagc                                                  >  8:125673/1‑144 (MQ=255)
                                                                                                      cGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTg                                                >  1:69868/1‑149 (MQ=255)
                                                                                                       gCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTgg                                               >  4:167228/1‑149 (MQ=255)
                                                                                                           gATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACt                                           <  3:179730/149‑1 (MQ=255)
                                                                                                             tACACTGATTTGCGTGTTCCTGTCGGTGTCTCTGGCGCTTGGCGCCAGCCTGCTTCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGATAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTgg                                         <  3:35736/149‑1 (MQ=255)
                                                                                                               cacTGATTGTCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGTACCAGCAAGTCCTGGGc                                       <  5:162300/149‑1 (MQ=255)
                                                                                                                acTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCa                                      >  3:281144/1‑149 (MQ=255)
                                                                                                                acTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCa                                      >  6:253826/1‑149 (MQ=255)
                                                                                                                acTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGAGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTCGGc                                       >  5:138317/1‑148 (MQ=255)
                                                                                                                 cTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTg                                                                                                                              <  4:254094/60‑1 (MQ=255)
                                                                                                                 cTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTg                                                                                                                              >  3:254094/1‑60 (MQ=255)
                                                                                                                 cTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCAt                                     <  2:323622/149‑1 (MQ=255)
                                                                                                                   gATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCAtcg                                   >  6:174987/1‑149 (MQ=255)
                                                                                                                   gATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCAtcg                                   >  7:76379/1‑149 (MQ=255)
                                                                                                                         cGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCgg                             <  2:235901/149‑1 (MQ=255)
                                                                                                                          gtgtTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCggt                            <  1:426875/149‑1 (MQ=255)
                                                                                                                                 tGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCggtgctggtg                     >  5:342060/1‑149 (MQ=255)
                                                                                                                                     ggTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCtt                  <  5:253826/148‑1 (MQ=255)
                                                                                                                                      gtgtCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGc                <  7:167784/149‑1 (MQ=255)
                                                                                                                                          cGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGTACCAGCAAGTCCTGGGCac                                     >  2:22032/1‑123 (MQ=255)
                                                                                                                                          cGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGTACCAGCAAGTCCTGGGCac                                     <  1:22032/124‑2 (MQ=255)
                                                                                                                                           gCTGGCGCCAAGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTgg           >  3:421111/1‑149 (MQ=255)
                                                                                                                                           gCTGGCGCCAAGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTg            <  6:435355/148‑1 (MQ=255)
                                                                                                                                           gCTGGCGCCAAGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTg            >  5:435355/1‑148 (MQ=255)
                                                                                                                                               gcgcTTGGCGCCAGCCTGCTGCTGATGCTTGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCACAGGACTTGCTGGTACTTGGCATCGTCGCGGTGCTGGTGCTTGCCTTGGCGCt       <  6:131988/149‑1 (MQ=255)
                                                                                                                                                cgcTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTGGCGCTg      >  6:59840/1‑149 (MQ=255)
                                                                                                                                                 gcTTGGGGGCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCAAATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTGGCGCTGc     <  6:138317/149‑1 (MQ=255)
                                                                                                                                                   ttGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTGGCGCTGCCg   >  3:379971/1‑149 (MQ=255)
                                                                                                                                                    tGGCGCCAGCCTACTGCTGATGCTGGCAGGCAAGATCACCGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCAGTGCTGGTGCTTGCCTTGGCGCTGCCGc  >  1:292575/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCCTGCTGGGTGAGCCCTATACCGGCCCTTACGGCAGCCTGTTCGGCTACTGCCTGCTGTTCGGCATCCTGCTCAACTTCCTGCGCAACCGTACCGGGCTGTCGCCGGATACACTGATTGGCGTGTTCCTGTCGGTGTCGCTGGCGCTTGGCGCCAGCCTGCTGCTGATGCTGGCAGGCAAGATCAACGTGCACATCCTCGAGAACGTGCTGTTCGGTTCGGTGCTGACCGTTAGTGCCCAGGACTTGCTGGTACTGGGCATCGTCGCGGTGCTGGTGCTTGCCTTGGCGCTGCCGC  >  NC_002947/4331518‑4331814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑