Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 304,429 C→T 5.2% R114H (CGC→CAC)  PP_0249 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947304,4290CT 5.2% 297.9 / 3.1 96R114H (CGC→CAC) PP_0249hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (53/38);  new base T (2/3);  total (55/41)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

GCGGCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCA  >  NC_002947/304287‑304575
                                                                                                                                              |                                                                                                                                                  
gCGGCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACt                                                                                                                                              >  7:137448/1‑149 (MQ=255)
  ggCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTg                                                                                                                                             >  3:23067/1‑148 (MQ=255)
  ggCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTg                                                                                                                                             >  4:415037/1‑148 (MQ=255)
  ggCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGc                                                                                                                                            <  6:445246/149‑1 (MQ=255)
  ggCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTCTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGc                                                                                                                                            <  4:77846/149‑1 (MQ=255)
   gCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGTGCagttgcg                                                                                                                                           >  2:41890/1‑143 (MQ=255)
        ccAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGTAGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCaccacc                                                                                                                                      >  1:305620/1‑149 (MQ=255)
        ccAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCaccacc                                                                                                                                      >  8:371333/1‑149 (MQ=255)
         cAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCaccacc                                                                                                                                      >  1:392023/1‑148 (MQ=255)
            ggTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGNAGCGGCGGTGGTGCAGTTGCGCCACCgccg                                                                                                                                  <  1:41890/149‑4 (MQ=255)
              tGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGGTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGTGCAGTTGCGCCACCgccgct                                                                                                                                <  1:172487/149‑6 (MQ=255)
                  cTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGt                                                                                                                            <  8:363707/149‑1 (MQ=255)
                   tCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTg                                                                                                                           >  8:87818/1‑149 (MQ=255)
                   tCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTg                                                                                                                           >  6:342972/1‑149 (MQ=255)
                     cACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCTCCCCGGGGCCCAGGTgcg                                                                                                                         >  4:76941/1‑149 (MQ=255)
                     cACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTgcg                                                                                                                         >  8:9018/1‑149 (MQ=255)
                     cACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCATCGAAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTgcg                                                                                                                         >  7:64623/1‑149 (MQ=255)
                     cACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCCTTGGAGCTCTTGATCGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCCCCCCCGAGCCCAGGTgcc                                                                                                                         >  7:25571/1‑148 (MQ=255)
                       ccTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCa                                                                                                                       <  8:233211/149‑1 (MQ=255)
                        cTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAg                                                                                                                      >  7:249814/1‑149 (MQ=255)
                           ccACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCTCCACCGAGCCCAGGTGCGCAGTg                                                                                                                    <  3:76941/148‑1 (MQ=255)
                           ccACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                    <  3:415037/148‑1 (MQ=255)
                           ccACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTg                                                                                                                    <  7:9018/148‑1 (MQ=255)
                            cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                  <  5:237441/149‑1 (MQ=255)
                            cacacaCCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACAGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                  <  8:99817/149‑1 (MQ=255)
                                 acCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGaa                                                                                                             <  4:23067/149‑1 (MQ=255)
                                 acCATCACCCAGCGCCAGGGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGaa                                                                                                             <  6:351034/149‑1 (MQ=255)
                                     tCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCa                                                                                                         <  8:218348/149‑1 (MQ=255)
                                       aCCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAgg                                                                                                       <  7:87818/149‑1 (MQ=255)
                                       aCCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAg                                                                                                        <  4:41760/148‑1 (MQ=255)
                                            gcgcCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTgg                                                                                                  <  5:342972/149‑1 (MQ=255)
                                            gcgcCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTgg                                                                                                  >  1:282859/1‑149 (MQ=255)
                                               ccAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCg                                                                                               >  6:396303/1‑149 (MQ=255)
                                                 aGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTc                                                                                             <  8:137448/149‑1 (MQ=255)
                                                     aCGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCgcgc                                                                                         <  8:249814/149‑1 (MQ=255)
                                                            gATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTTGCCGTCGCGCTCGAAgg                                                                                  >  4:258061/1‑149 (MQ=255)
                                                            gATGACGTAGCGGGTCTGGGCGTCGCCGTTGGAGATCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCACCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCACGGCTGGCCGTCGCGATCCAAgg                                                                                  >  2:82685/1‑149 (MQ=255)
                                                             aTGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                  >  6:242279/1‑116 (MQ=255)
                                                             aTGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGaa                                                                                                                  <  5:242279/116‑1 (MQ=255)
                                                                 cGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTcc                                                                             >  8:401801/1‑149 (MQ=255)
                                                                   tAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCgcgc                                                                                         >  4:9909/1‑135 (MQ=255)
                                                                   tAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCgcgc                                                                                         <  3:9909/135‑1 (MQ=255)
                                                                       gggTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCg                                                                       <  7:401801/149‑1 (MQ=255)
                                                                        ggTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCgg                                                                      <  8:64623/149‑1 (MQ=255)
                                                                         gTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGt                                                                     >  3:159603/1‑149 (MQ=255)
                                                                          tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                    >  2:104498/1‑149 (MQ=255)
                                                                          tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                    >  1:51846/1‑149 (MQ=255)
                                                                          tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                    >  8:52196/1‑149 (MQ=255)
                                                                          tCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGACCGTCGCGCTCGAAGGGTTCCACTTCGGTg                                                                    >  6:359933/1‑149 (MQ=255)
                                                                            tGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGc                                                                  >  8:66490/1‑149 (MQ=255)
                                                                             gtgtgTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGc                                                                  <  1:159001/144‑1 (MQ=255)
                                                                              ggCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCAt                                                                >  7:262691/1‑149 (MQ=255)
                                                                                 gTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCa                                                             <  5:396303/149‑1 (MQ=255)
                                                                                 gTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGAAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATg                                                               >  4:116918/1‑147 (MQ=255)
                                                                                    gCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGc                                                          >  4:84079/1‑149 (MQ=255)
                                                                                    gCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGc                                                          >  4:75461/1‑149 (MQ=255)
                                                                                     ccGTTGGAGCTCTTGATGGTTTTCATCGTCACCATCGGGGCCTCGCAGCGGCGGTGGCGCAACTTCACCACCGAGACCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGAATGCAGGCt                                                         <  8:25571/149‑1 (MQ=255)
                                                                                          ggAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGAc                                                     <  3:258061/148‑1 (MQ=255)
                                                                                           gAGCTCTTGATGGTTTTCATCGTCACCAACTGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGt                                                   >  1:260327/1‑149 (MQ=255)
                                                                                            aGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGc                                                                  <  5:245415/133‑1 (MQ=255)
                                                                                            aGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGc                                                                  >  6:245415/1‑133 (MQ=255)
                                                                                              ctctTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCt                                                >  5:396621/1‑149 (MQ=255)
                                                                                               tctTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                               >  8:162441/1‑149 (MQ=255)
                                                                                               tctTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                               >  1:332844/1‑149 (MQ=255)
                                                                                               tctTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTg                                               >  8:266959/1‑149 (MQ=255)
                                                                                                   gATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTgg                                              <  3:62702/146‑1 (MQ=255)
                                                                                                   gATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCa                                            <  7:52196/148‑1 (MQ=255)
                                                                                                   gATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAc                                           >  4:405436/1‑149 (MQ=255)
                                                                                                    aTGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAcc                                          >  4:387464/1‑149 (MQ=255)
                                                                                                    aTGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAcc                                          >  4:31572/1‑149 (MQ=255)
                                                                                                    aTGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCAc                                           <  2:260327/148‑1 (MQ=255)
                                                                                                     tGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCACGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCg                                         >  6:253026/1‑149 (MQ=255)
                                                                                                             aTCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGAt                                 >  6:122126/1‑149 (MQ=255)
                                                                                                              tCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATTCAGGCTCTACGTGCTGGCACCGGTGTCGAt                                 <  7:66490/148‑1 (MQ=255)
                                                                                                              tCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATc                                >  5:1004/1‑149 (MQ=255)
                                                                                                              tCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATc                                >  5:342765/1‑149 (MQ=255)
                                                                                                              tCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGACCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATc                                <  5:359933/149‑1 (MQ=255)
                                                                                                                 tCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTg                             <  2:332844/149‑1 (MQ=255)
                                                                                                                  cACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTgg                            <  3:387464/149‑1 (MQ=255)
                                                                                                                    ccAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcg                          >  3:56466/1‑149 (MQ=255)
                                                                                                                     cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                         >  1:390542/1‑149 (MQ=255)
                                                                                                                     cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                         >  4:67606/1‑149 (MQ=255)
                                                                                                                     cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                         >  2:347657/1‑149 (MQ=255)
                                                                                                                     cAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAAAGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgc                         >  1:126333/1‑149 (MQ=255)
                                                                                                                      aaCGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGgcgcg                        >  1:55046/1‑149 (MQ=255)
                                                                                                                               tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc               >  3:110087/1‑149 (MQ=255)
                                                                                                                               tCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGc               >  1:241924/1‑149 (MQ=255)
                                                                                                                                cGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGcc              >  3:156763/1‑149 (MQ=255)
                                                                                                                                 gCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGcc              <  6:1004/148‑1 (MQ=255)
                                                                                                                                    gcggcgGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGc          <  4:156763/149‑1 (MQ=255)
                                                                                                                                       tcggTGGTGCAGTTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCAc            <  2:398057/143‑1 (MQ=255)
                                                                                                                                       tcggTGGTGCAGTTGCGCCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCAc            >  1:398057/2‑144 (MQ=255)
                                                                                                                                       gcggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGa       <  4:159603/149‑1 (MQ=255)
                                                                                                                                        cggTGGCGCAACTGCACCAACGAGCCCAGGTGCGCAGGGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAg      <  2:305620/149‑1 (MQ=255)
                                                                                                                                         ggTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAgg     >  8:202114/1‑149 (MQ=255)
                                                                                                                                           tGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTc   <  5:122126/149‑1 (MQ=255)
                                                                                                                                             gcgcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTca  <  3:87682/148‑1 (MQ=255)
                                                                                                                                             gcgcAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTca  <  2:241924/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GCGGCAGGCCAGGGTGAACTCCACCTCCCACACACCATCACCCAGCGCCAGTGACGTGCGGATGACGTAGCGGGTCTGGGCGTGGCCGTTGGAGCTCTTGATGGTTTTCATCGTCACCAACGGTGCCTCGCAGCGGCGGTGGCGCAACTGCACCACCGAGCCCAGGTGCGCAGTGAAGCGAACCCAGGGCTGGCCGTCGCGCTCGAAGGGTTCCACTTCGGTGGCATGCAGGCTCGACGTGCTGGCACCGGTGTCGATCTTGGCGCGCAGCCCGGCCACGCCGAGGTCA  >  NC_002947/304287‑304575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑