Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,363,969	T→G	7.1%	intergenic (+44/‑30)	adhP → / → PP_3840	alcohol dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,363,969	0	T	G	7.1%	201.3 / 4.2	66	intergenic (+44/‑30)	adhP/PP_3840	alcohol dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base G (3/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGCGA  >  NC_002947/4363824‑4364114                                                                                                                                                  |                                                                                                                                                   cccAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGa                                                                                                                                                  <  5:33882/148‑1 (MQ=255)                 gACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCttt                                                                                                                                   >  3:258403/1‑147 (MQ=255)                    cGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGcc                                                                                                                              >  2:108448/1‑149 (MQ=255)                    cGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGcc                                                                                                                              <  7:230118/149‑1 (MQ=255)                    cGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGCAGAGGGTCATCTACGGGCtttgctgt                                                                                                                              <  4:151183/149‑6 (MQ=38)                             atatCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                     >  7:257727/1‑149 (MQ=255)                             atatCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTAACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAAtt                                                                                                                     <  3:228866/149‑1 (MQ=255)                               atCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTg                                                                                                                    >  7:246963/1‑148 (MQ=255)                                tCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑Tc                                                                                                                 <  4:398999/149‑1 (MQ=255)                                 cAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGAt                                                                                                                                                    >  3:56290/1‑114 (MQ=255)                                 cAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGAt                                                                                                                                                    <  4:56290/114‑1 (MQ=255)                                 cAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑Tc                                                                                                                 >  6:119693/1‑148 (MQ=255)                                 cAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑Tc                                                                                                                 >  5:231197/1‑148 (MQ=255)                                    ccAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAg                                                                                                             >  7:312603/1‑149 (MQ=255)                                     cAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAg                                                                                                             >  4:57057/1‑148 (MQ=255)                                       gATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGaa                                                                                                          >  2:264943/1‑149 (MQ=255)                                           tttGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGa                                                                                                      >  4:378201/1‑149 (MQ=255)                                             tGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACa                                                                                                        <  4:258403/145‑1 (MQ=255)                                               aCGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAg                                                                                                                                >  8:390115/1‑120 (MQ=255)                                               aCGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAg                                                                                                                                <  7:390115/120‑1 (MQ=255)                                               aCGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCAt                                                                                                  >  8:104611/1‑149 (MQ=255)                                                cGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCAt                                                                                                  >  8:196334/1‑148 (MQ=255)                                                 gagaTGCTGGAGGGCAAGATCAAAGGCCTGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGa                                                                                                <  4:156714/149‑1 (MQ=255)                                                 gagaTGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGa                                                                                                <  2:129145/149‑1 (MQ=255)                                                   gaTGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGAcc                                                                                              >  8:50957/1‑149 (MQ=255)                                                    aTGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCg                                                                                             >  5:79583/1‑149 (MQ=255)                                                                    tCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCgg                                                                             >  1:385260/1‑149 (MQ=255)                                                                      aaaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGc                                                                           >  5:235186/1‑149 (MQ=255)                                                                       aaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGcc                                                                          <  8:246963/149‑1 (MQ=255)                                                                       aaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGcc                                                                          >  4:97714/1‑149 (MQ=255)                                                                       aaGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGcc                                                                          <  7:196334/149‑1 (MQ=255)                                                                             ggATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAg                                                                    >  6:417398/1‑149 (MQ=255)                                                                             ggATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAg                                                                    <  6:64144/149‑1 (MQ=255)                                                                              gATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGc                                                                   <  3:97714/149‑1 (MQ=255)                                                                                    gATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTGCTCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCTGATTAGCCGCa                                                               <  1:108448/148‑1 (MQ=255)                                                                                    gATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCAc                                                              >  6:262870/1‑148 (MQ=255)                                                                                      tACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGAc                                                           <  8:257727/149‑1 (MQ=255)                                                                                       aCAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACt                                                          >  2:345080/1‑149 (MQ=255)                                                                                        cAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTc                                                         <  2:385260/149‑1 (MQ=255)                                                                                          gTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCa                                                       >  5:164303/1‑149 (MQ=255)                                                                                           ttCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCAc                                                      <  7:50957/149‑1 (MQ=255)                                                                                           ttCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGAACCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCAc                                                      >  4:75066/1‑149 (MQ=255)                                                                                              gAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTg                                                    <  3:57057/148‑1 (MQ=255)                                                                                               aaGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCAGGCCGATTAGCCGCACGACTCCACTGGa                                                  >  8:222871/1‑149 (MQ=255)                                                                                                  gCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCggg                                                                            <  5:15932/120‑1 (MQ=255)                                                                                                  gCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCggg                                                                            >  6:15932/1‑120 (MQ=255)                                                                                                   cTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAg                                              <  5:262870/149‑1 (MQ=255)                                                                                                    tGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAg                                              >  6:51986/1‑148 (MQ=255)                                                                                                        aGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTa                                         >  4:391060/1‑149 (MQ=255)                                                                                                        aGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTa                                         >  8:308868/1‑149 (MQ=255)                                                                                                          gCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTAcc                                       <  8:312603/149‑1 (MQ=255)                                                                                                                  aGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACg                               >  4:288004/1‑149 (MQ=255)                                                                                                                   gggcATGTGGAACCACAGCAAAGCAGGTAGAGGACCCTCTGCGGGGTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCTCACGACTCCACTGGACAAGCATTACCGCAAGACGc                              <  8:316531/146‑1 (MQ=255)                                                                                                                    gTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACg                               >  1:332559/1‑147 (MQ=255)                                                                                                                            gAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGtt                      <  5:417398/148‑1 (MQ=255)                                                                                                                              aCCACAGCAAAGCCCGTAGATGACCAGCTGCGGGCTTTTAGCCTTATTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGt                   <  5:112385/149‑1 (MQ=255)                                                                                                                               ccACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTc                  <  3:378201/149‑1 (MQ=255)                                                                                                                                  gctaaaaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGt               >  1:212390/5‑149 (MQ=255)                                                                                                                                     aaaaGCCCGCAGAGGGTCATCTAC‑GGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCt           >  4:412266/2‑149 (MQ=255)                                                                                                                                      aaaGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCt           >  3:74162/1‑149 (MQ=255)                                                                                                                                       aaGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTg          <  5:119693/149‑1 (MQ=255)                                                                                                                                       aaGCCCGCAGAGGGTCATCTACGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACAAGCAGAAGGTTCGTCAGTTTCTg          >  8:200238/1‑149 (MQ=255)                                                                                                                                        aGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGc         <  3:54131/149‑1 (MQ=255)                                                                                                                                         gCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTcc                                                        >  4:277260/1‑101 (MQ=255)                                                                                                                                         gCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTcc                                                        <  3:277260/101‑1 (MQ=255)                                                                                                                                            cGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAg     <  2:332559/149‑1 (MQ=255)                                                                                                                                             gTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGc    <  6:231197/149‑1 (MQ=255)                                                                                                                                                gATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGCGa  <  3:75066/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   CCCAAGGTAACGCTGCGACCGATCGAGGATATCAACCAGATCTTTGACGAGATGCTGGAGGGCAAGATCAAAGGCCGGATGGTGATACAGTTCGAAGGCTGACAGGCCGAGCCAGGTCATGTGGAACCACAGCAAAGCCCGTAGATGACCCTCTGCGGGCTTTTAGCCTTCTTAATTG‑TCAAAGGAACAGACCATGACCGTCGACTGGCACGCCGGGCCGATTAGCCGCACGACTCCACTGGACAAGCATTACCGCAACACGCAGAAGGTTCGTCAGTTTCTGCTCAGCGA  >  NC_002947/4363824‑4364114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑