Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,442,629 C→G 9.3% intergenic (‑99/+39) nicP‑II ← / ← nicT porin‑like protein/metabolite transport protein NicT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,442,6290CG 9.3% 262.8 / 14.7 86intergenic (‑99/+39)nicP‑II/nicTporin‑like protein/metabolite transport protein NicT
Reads supporting (aligned to +/- strand):  ref base C (41/37);  new base G (5/3);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GGCCAGGCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGTGA  >  NC_002947/4442483‑4442777
                                                                                                                                                  |                                                                                                                                                    
ggCCAGGCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCgg                                                                                                                                                    <  7:131713/149‑1 (MQ=255)
 gCCAGGCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGc                                                                                                                                                   >  4:151192/1‑149 (MQ=255)
     ggCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGc                                                                                                                                               <  8:184564/149‑1 (MQ=255)
     ggCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGc                                                                                                                                               <  8:63998/149‑1 (MQ=255)
        gTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcctg                                                                                                                                            <  6:225776/149‑1 (MQ=255)
        gTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcctg                                                                                                                                            <  8:252287/149‑1 (MQ=255)
        gTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcctg                                                                                                                                            >  1:335104/1‑149 (MQ=255)
        gTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcctg                                                                                                                                            <  4:373068/149‑1 (MQ=255)
           cAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcctgcc                                                                                                                                          <  5:434801/148‑1 (MQ=255)
               cGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCctgccctgcc                                                                                                                                     >  3:151296/1‑149 (MQ=255)
                  agaaAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGtt                                                                                                                                  >  8:105992/1‑149 (MQ=255)
                     aaaaCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGc                                                                                                                                <  4:255830/148‑1 (MQ=255)
                      aaaCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGt                                                                                                                              <  8:73059/149‑1 (MQ=255)
                      aaaCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGt                                                                                                                              <  1:16206/149‑1 (MQ=255)
                      aaaCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGt                                                                                                                              <  7:94269/149‑1 (MQ=255)
                      aaaCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCAACGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGt                                                                                                                              <  4:81347/149‑1 (MQ=255)
                               gcAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTg                                                                                                                     >  4:277403/1‑149 (MQ=255)
                                   tGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGcc                                                                                                                 >  4:207054/1‑149 (MQ=255)
                                     aaaCCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGc                                                                                                               <  7:105992/149‑1 (MQ=255)
                                            gCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGACGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCg                                                                                                          <  3:387443/147‑1 (MQ=255)
                                              aTAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGtt                                                                                                       >  5:311540/1‑148 (MQ=255)
                                              aTAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTg                                                                                                      <  3:151192/149‑1 (MQ=255)
                                                     tACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCt                                                                                               >  3:346441/1‑149 (MQ=255)
                                                     tACTCCTGGATTTGGGCAACAGCAACCCGGCGCAGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCt                                                                                               >  2:132082/1‑149 (MQ=255)
                                                       cTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCt                                                                                               <  5:73802/147‑1 (MQ=255)
                                                        tCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTgg                                                                                            >  2:365571/1‑149 (MQ=255)
                                                         ccTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGc                                                                                           >  5:142314/1‑149 (MQ=255)
                                                          cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTgg                                                                                            <  4:67205/147‑1 (MQ=255)
                                                          cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTgg                                                                                            >  3:67205/1‑147 (MQ=255)
                                                          cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGcc                                                                                          >  5:98939/1‑149 (MQ=255)
                                                          cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGcc                                                                                          >  4:123513/1‑149 (MQ=255)
                                                           tGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCg                                                                                         >  8:377660/1‑149 (MQ=255)
                                                           tGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCg                                                                                         >  5:130212/1‑149 (MQ=255)
                                                           tGGATCTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCg                                                                                         >  6:308810/1‑149 (MQ=255)
                                                             gATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTGGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCgg                                                                                        <  6:311540/148‑1 (MQ=255)
                                                                  gggCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGGTGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAgg                                                                                   <  2:165953/148‑1 (MQ=255)
                                                                  gggCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAgg                                                                                   <  8:284768/148‑1 (MQ=255)
                                                                   ggCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGTCGGCAAGGCg                                                                                 <  2:335104/149‑1 (MQ=255)
                                                                    gCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGa                                                                                >  8:295137/1‑149 (MQ=255)
                                                                     cAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGAt                                                                               <  6:271924/149‑1 (MQ=255)
                                                                      aaCAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGAt                                                                               >  1:115694/1‑148 (MQ=255)
                                                                      aaCAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATg                                                                              >  4:316758/1‑149 (MQ=255)
                                                                        cAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGAc                                                                            <  6:142314/149‑1 (MQ=255)
                                                                         aGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGAc                                                                            >  7:8527/1‑148 (MQ=255)
                                                                           cAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGGTGACCAGCTTGGCCGGCAAGGCGATGACCAg                                                                         >  7:75309/1‑149 (MQ=255)
                                                                           cAACCCGGCACGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAg                                                                         >  6:208117/1‑149 (MQ=255)
                                                                              cccGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGaa                                                                      <  1:365571/149‑1 (MQ=255)
                                                                                cggcgcggcgGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCa                                                                          >  3:17881/1‑143 (MQ=255)
                                                                                cggcgcggcgGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCa                                                                          <  4:17881/143‑1 (MQ=255)
                                                                                 ggcgcggcgGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAagca                                                                   <  3:316758/149‑1 (MQ=255)
                                                                                      ggcggcACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTGGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCt                                                                >  8:93527/1‑147 (MQ=255)
                                                                                       gcggcACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAg                                                             <  7:295137/149‑1 (MQ=255)
                                                                                        cggcACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGa                                                              <  1:132082/147‑1 (MQ=255)
                                                                                          gcACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGAt                                                          >  2:243045/1‑149 (MQ=255)
                                                                                           cACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATc                                                         <  8:8527/149‑1 (MQ=255)
                                                                                            aCTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCa                                                        <  5:308810/149‑1 (MQ=255)
                                                                                            aCTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCa                                                        <  3:207054/149‑1 (MQ=255)
                                                                                                       aCCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGc                                              >  2:85828/1‑148 (MQ=255)
                                                                                                        ccGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCaa                                            <  6:98939/149‑1 (MQ=255)
                                                                                                                 gggTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCcaca                                   >  1:197574/1‑149 (MQ=255)
                                                                                                                   gTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcagc                                 <  1:243045/149‑1 (MQ=255)
                                                                                                                        aTCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcagcgcagc                            >  1:53920/1‑149 (MQ=255)
                                                                                                                        aTCGAGAGAGGTCGGCAGGCGGGCCCCCGCCCCCCAGCCCAGCCGTTGGGTGAAGCGCTGGGCCGCGAGCGGGTGACCAGCTTTGCCGGCAAGGCGATGACCACGAAGCAGCAGAGGATCAGGCAGCCCGCAAACACCCAcaccccagc                            >  2:203043/1‑149 (MQ=255)
                                                                                                                            agagagGTACGCAGCCGGGACCCCGCCCGCCTGCCCAGACGTTGCGATAAGCGCTGGGCCGCAAGCGGTTGACCAGCTTGGCCGGCAACGCGATGACCACGAAGCAGCGGAGGATCACGCAGCCGGCAAACCACCAcagcgcagctacc                        >  6:441005/1‑145 (MQ=255)
                                                                                                                             gagagGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCg                       >  7:285219/1‑149 (MQ=255)
                                                                                                                                agGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAg                     <  4:346441/148‑1 (MQ=255)
                                                                                                                                    cGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGcc                <  5:208117/149‑1 (MQ=255)
                                                                                                                                     ggCAGGCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCg               >  2:353864/1‑149 (MQ=255)
                                                                                                                                     ggCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGCCCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCg               >  3:351162/1‑149 (MQ=255)
                                                                                                                                     ggCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCg               >  6:305047/1‑149 (MQ=255)
                                                                                                                                       cAGGCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGt             >  5:171473/1‑149 (MQ=255)
                                                                                                                                       cAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGt             >  6:252830/1‑149 (MQ=255)
                                                                                                                                        aGGCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcagcgcagc                            <  6:442774/133‑1 (MQ=255)
                                                                                                                                        aGGCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcagcgcagc                            >  5:442774/1‑133 (MQ=255)
                                                                                                                                        aGGCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGt             >  2:318201/1‑148 (MQ=255)
                                                                                                                                        aGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGt             >  2:135044/1‑148 (MQ=255)
                                                                                                                                         ggCGGGCCGGGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGt             >  7:405859/1‑147 (MQ=255)
                                                                                                                                          gCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAg          <  1:203043/149‑1 (MQ=255)
                                                                                                                                          gCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAg          <  2:115694/149‑1 (MQ=255)
                                                                                                                                          gCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAg          >  4:315577/1‑149 (MQ=255)
                                                                                                                                           cGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCcac                                    <  2:383061/122‑1 (MQ=255)
                                                                                                                                           cGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCcac                                    >  1:383061/1‑122 (MQ=255)
                                                                                                                                           cGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAgg         >  3:89673/1‑149 (MQ=255)
                                                                                                                                            gggCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGa           <  4:88980/146‑1 (MQ=255)
                                                                                                                                            gggCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGa           >  3:88980/1‑146 (MQ=255)
                                                                                                                                             ggCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCCGCGTGCGAGGTGCCGGTGAGGTc       >  7:133763/1‑149 (MQ=255)
                                                                                                                                               ccccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCgg     <  3:277403/149‑1 (MQ=255)
                                                                                                                                               ccccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCg      >  8:178128/1‑148 (MQ=255)
                                                                                                                                               ccccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCg      <  7:178128/148‑1 (MQ=255)
                                                                                                                                                cccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGt    <  5:63341/149‑1 (MQ=255)
                                                                                                                                                cccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGt    <  2:197574/149‑1 (MQ=255)
                                                                                                                                                cccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGt    <  2:53920/149‑1 (MQ=255)
                                                                                                                                                 ccGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGt    >  6:413912/1‑148 (MQ=255)
                                                                                                                                                  cGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGTGa  <  7:377660/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGCCAGGCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCTGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGTGA  >  NC_002947/4442483‑4442777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑