Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,468,367 G→T 5.3% A204A (GCG→GCT)  PP_3959 → voltage‑gated chloride channel family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,468,3670GT 5.3% 229.7 / 3.6 75A204A (GCG→GCT) PP_3959voltage‑gated chloride channel family protein
Reads supporting (aligned to +/- strand):  ref base G (35/36);  new base T (2/2);  total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01

GGCGCACCATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGGGTGGTTTTTACG  >  NC_002947/4468222‑4468512
                                                                                                                                                 |                                                                                                                                                 
ggcGCACCATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTg                                                                                                                                                <  4:422949/149‑1 (MQ=255)
ggcGCACCATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTg                                                                                                                                                >  5:391640/1‑149 (MQ=255)
 gcgcACCATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGc                                                                                                                                               <  6:175043/149‑1 (MQ=255)
    cACCATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGa                                                                                                                                            <  3:95934/149‑1 (MQ=255)
      ccATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACAACGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATc                                                                                                                                          >  5:305825/1‑149 (MQ=255)
       cATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATcg                                                                                                                                         >  5:244516/1‑149 (MQ=255)
       cATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATcg                                                                                                                                         >  6:406554/1‑149 (MQ=255)
               gCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTc                                                                                                                                 <  2:191941/149‑1 (MQ=255)
                    gTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGa                                                                                                                             <  1:19388/148‑1 (MQ=255)
                    gTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGa                                                                                                                             <  7:210928/148‑1 (MQ=255)
                       cTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCt                                                                                                                         <  1:206260/149‑1 (MQ=255)
                         aGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCTGTGCTGATCGGCGGAGTCGTGACGCTgg                                                                                                                       >  3:162763/1‑149 (MQ=255)
                         aGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCTGTGCTGATCGGCGGAGTCGTGACGCTgg                                                                                                                       >  3:156406/1‑149 (MQ=255)
                            cGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTGTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGc                                                                                                                    >  5:303814/1‑149 (MQ=255)
                                 gcgcTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATg                                                                                                               <  6:374770/149‑1 (MQ=255)
                                     tGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATgggg                                                                                                            >  5:333634/1‑148 (MQ=255)
                                         ggTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGGGGAGTCGTTCCGCTGGGTCTGATGGGGCAtt                                                                                                        >  6:300800/1‑148 (MQ=255)
                                          gTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTAc                                                                                                      >  1:200052/1‑149 (MQ=255)
                                               gCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTa                                                                                                 >  2:116862/1‑149 (MQ=255)
                                                      ccTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTAACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCATACTTTGGc                                                                                          >  1:144833/1‑149 (MQ=255)
                                                        ttCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTCCTTTGGc                                                                                          >  6:102418/1‑147 (MQ=255)
                                                        ttCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGc                                                                                          >  5:381319/1‑147 (MQ=255)
                                                        ttCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATAGGCGGAGTCGAGAAGCTTGGGCTGATGGGGCATTAAGCCTACTTTGGc                                                                                          >  5:192233/1‑147 (MQ=255)
                                                         ttaaTACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGc                                                                                          >  7:175057/3‑146 (MQ=255)
                                                         tCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGc                                                                                          <  2:259191/146‑1 (MQ=255)
                                                         tCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGc                                                                                          >  1:259191/1‑146 (MQ=255)
                                                           aaTACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATgggg                                                                                                            <  2:317182/126‑1 (MQ=255)
                                                           aaTACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATgggg                                                                                                            >  1:317182/1‑126 (MQ=255)
                                                                acCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCg                                                                                <  5:406554/149‑1 (MQ=255)
                                                                acCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCg                                                                                >  1:2498/1‑149 (MQ=255)
                                                                acCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCg                                                                                >  4:33654/1‑149 (MQ=255)
                                                                    ttAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCTGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCg                                                                            <  4:156406/149‑1 (MQ=255)
                                                                    ttAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCTGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCg                                                                            <  4:162763/149‑1 (MQ=255)
                                                                           gggTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCg                                                                     >  6:118876/1‑149 (MQ=255)
                                                                                   tttGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTg                                                             >  6:259386/1‑149 (MQ=255)
                                                                                   tttGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGAGCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGAGGAGCGGGCGGATGTCGGTGCACTg                                                             >  4:377972/1‑149 (MQ=255)
                                                                                     tGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTgg                                                            <  6:391640/148‑1 (MQ=255)
                                                                                     tGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTg                                                             <  6:333634/147‑1 (MQ=255)
                                                                                     tGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTg                                                             <  7:4666/147‑1 (MQ=255)
                                                                                      gCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACg                                                                                                     <  2:355998/106‑1 (MQ=255)
                                                                                      gCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACg                                                                                                     >  1:355998/1‑106 (MQ=255)
                                                                                       ccATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGGTGCCGGTGGACTGGGGc                                                         >  7:265042/1‑149 (MQ=255)
                                                                                        cATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACACCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCt                                                        >  2:57916/1‑149 (MQ=255)
                                                                                          tttaGGAATTGAGCCGGAGGTTCGTGCAAGGTTGCAGTGGCTTGGTGCGCACTGCGTTGCTGATCGGCGGAGTCGGGACGCTGTGGATGAGGGGGCATTACTCCTACTTTGGCGAGAGCATCGGTCGGATGCCGGTGGACTGGTGCTgg                                                      <  6:192233/146‑1 (MQ=255)
                                                                                          ttGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTgg                                                      >  7:328566/1‑149 (MQ=255)
                                                                                             aGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGc                                                   <  2:200052/149‑1 (MQ=255)
                                                                                               gAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGGGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGcgt                                                 >  5:371339/1‑149 (MQ=255)
                                                                                                aaTTGAGCCGCACGTTCGAGCAACGTTTCAGTTGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGcgtc                                                <  6:305825/149‑1 (MQ=255)
                                                                                                      gCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTcct                                          <  6:244516/149‑1 (MQ=255)
                                                                                                       gcGCACGTTCGTGCAACGTTTCAGTGGCTTGGTGCTGAATGCGTTTCTGATCGGCGGAGTCGTGTCGCTTTGGCTGATGGGTCATTTCGCGTACTTTGGGGTGTTCAGCTGTCGTATGTGGGTTGACTGGGGGTGGAGATTCGTTcctg                                         <  2:381267/148‑1 (MQ=255)
                                                                                                         gCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTcctgcc                                       >  7:140362/1‑149 (MQ=255)
                                                                                                          cACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTcct                                          <  6:177940/145‑1 (MQ=255)
                                                                                                          cACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTcct                                          >  5:177940/1‑145 (MQ=255)
                                                                                                             gTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCtgtg                                   >  7:188277/1‑149 (MQ=255)
                                                                                                                     aaCGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTg                              <  6:352817/146‑1 (MQ=255)
                                                                                                                     aaCGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTg                              >  5:352817/1‑146 (MQ=255)
                                                                                                                      aCGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTTCTTTGGCGAGATCAGCTGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTTCTgg                          <  5:300800/149‑1 (MQ=255)
                                                                                                                      aCGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGGTGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgg                          <  8:265042/149‑1 (MQ=255)
                                                                                                                      aCGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgg                          >  1:1843/1‑149 (MQ=255)
                                                                                                                         gttCAGTGGCTGGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcg                       <  6:303814/148‑1 (MQ=255)
                                                                                                                          ttCAGTGGTTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcg                       <  8:140362/148‑1 (MQ=255)
                                                                                                                           tCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcggg                     >  3:22990/1‑149 (MQ=255)
                                                                                                                            cAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcgggc                    >  2:61847/1‑149 (MQ=255)
                                                                                                                            cAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcggg                     <  6:381319/148‑1 (MQ=255)
                                                                                                                             aGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcgggc                    <  1:116862/148‑1 (MQ=255)
                                                                                                                             aGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACACCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGTGCGGCGTTCCTGCCTGTGCAGTGCTgggcgggc                    <  1:57916/148‑1 (MQ=255)
                                                                                                                                 gCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGctgctg               <  8:188277/149‑1 (MQ=255)
                                                                                                                                     ggTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcgg                      >  8:279404/1‑138 (MQ=255)
                                                                                                                                     ggTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGggtg           >  3:145879/1‑149 (MQ=255)
                                                                                                                                     ggTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGAGCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTgggcgg                      <  7:279404/138‑1 (MQ=255)
                                                                                                                                      gTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGggtg           <  3:172372/148‑1 (MQ=255)
                                                                                                                                        gCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCGGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGGGTGGtt        <  8:175057/149‑1 (MQ=255)
                                                                                                                                            aCTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGGCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGGGTGGTTTTTa    <  5:118876/149‑1 (MQ=255)
                                                                                                                                              tGCGGGGCTGATCGGCTGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACGTTGGCGAGATCAGCGGTCGGATGTCGGGGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGGGTGGTTTTTa    <  3:377972/147‑1 (MQ=255)
                                                                                                                                                cGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGGGTGGTTTTTACg  <  1:61847/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGCGCACCATCGCTGGCCTGGTGCTAGCCGGAGGCGCTGCGGGTATTGCGGCAGCCTTCAATACACCGTTAGCGGGGGTAGTCTTTGCCATTGAGGAATTGAGCCGCACGTTCGAGCAACGTTTCAGTGGCTTGGTGCTCACTGCGGTGCTGATCGGCGGAGTCGTGACGCTGGGGCTGATGGGGCATTACGCCTACTTTGGCGAGATCAGCGGTCGGATGCCGGTGGACTGGGGCTGGAGCGTCGTTCCTGCCTGTGCAGTGCTGGGCGGGCTGCTGGGTGGTTTTTACG  >  NC_002947/4468222‑4468512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑