Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,488,991 A→G 7.6% intergenic (+344/+227) PP_3981 → / ← PP_3982 transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,488,9910AG 7.6% 178.6 / 4.1 66intergenic (+344/+227)PP_3981/PP_3982transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (2/3);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

AAGATGCCCAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGTTTCAC  >  NC_002947/4488844‑4489131
                                                                                                                                                   |                                                                                                                                            
aaGATGCCCAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGaa                                                                                                                                              <  5:274839/148‑1 (MQ=255)
  gATGCCCAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCt                                                                                                                                           >  4:33206/1‑149 (MQ=255)
  gATGCCCAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCt                                                                                                                                           >  6:398715/1‑149 (MQ=255)
     gCCCAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTccc                                                                                                                                        >  2:148758/1‑149 (MQ=255)
      cccAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCg                                                                                                                                       <  8:10076/149‑1 (MQ=255)
      cccAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCg                                                                                                                                       >  6:151751/1‑149 (MQ=255)
       ccAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATTTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGc                                                                                                                                      >  7:44830/1‑149 (MQ=255)
       ccAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGc                                                                                                                                      >  6:386657/1‑149 (MQ=255)
        cAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCATATGGGCGGGAGGTTCCCGcc                                                                                                                                     >  7:406664/1‑149 (MQ=255)
           ggCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTCCTTGCACACGGGCGGGAGGTTCCCGcccgt                                                                                                                                  <  1:37847/149‑3 (MQ=255)
             cTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCATATGGGCGGGAGGTTCCCGcccgtgt                                                                                                                                <  8:406664/149‑5 (MQ=255)
             cTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAGGTTCCCGcccgtgt                                                                                                                                <  5:345245/149‑5 (MQ=255)
              tCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATg                                                                                                                               >  2:165603/1‑149 (MQ=255)
                 aCATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATgcgc                                                                                                                            <  1:14878/149‑1 (MQ=255)
                  cATTGCGCCATCACGACTGGAAAATGAAGGTTGTCGAAAATCCACCGATCGCCAAGATCAATCATCGAGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTCAGGCCTTGATTGCACACGGGAGGGACCCTCCCGCCCATATgcgc                                                                                                                            >  4:280045/1‑148 (MQ=255)
                      gcgcCATGACGACTGGAAACTGCAGGAAGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTAc                                                                                                                        <  2:81226/148‑1 (MQ=255)
                                  cTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATgc                                                                                                                              <  7:217503/130‑1 (MQ=255)
                                  cTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATgc                                                                                                                              >  8:217503/1‑130 (MQ=255)
                                      aaaaTGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATTTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATc                                                                                                       <  8:44830/149‑1 (MQ=255)
                                               gTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATa                                                                                                                  >  4:227880/1‑129 (MQ=255)
                                               gTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATa                                                                                                                  <  3:227880/129‑1 (MQ=255)
                                               gTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTcc                                                                                                 >  5:98878/1‑146 (MQ=255)
                                                  gTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGAc                                                                                            <  3:174395/148‑1 (MQ=255)
                                                     gAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTca                                                                                        >  4:403113/1‑149 (MQ=255)
                                                     gAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTca                                                                                        <  5:339382/149‑1 (MQ=255)
                                                       aaaTCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTcaca                                                                                      >  1:276555/1‑149 (MQ=255)
                                                          tCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACAtct                                                                                   >  3:152835/1‑149 (MQ=255)
                                                           ccACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACAtct                                                                                   >  7:207194/1‑148 (MQ=255)
                                                               cGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACtt                                                                              <  3:33206/149‑1 (MQ=255)
                                                                      cAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGaa                                                                       >  4:232282/1‑149 (MQ=255)
                                                                          aTCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTgg                                                                   <  5:386657/149‑1 (MQ=255)
                                                                                cATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTa                                                              >  6:62427/1‑148 (MQ=255)
                                                                                   cgcgCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCAc                                                                                >  8:301569/1‑127 (MQ=255)
                                                                                   cgcgCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCAc                                                                                <  7:301569/127‑1 (MQ=255)
                                                                                      gcCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAActct                                                       <  1:148758/149‑1 (MQ=255)
                                                                                        ccAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTcc                                                     <  5:398715/149‑1 (MQ=255)
                                                                                            cGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAg                                                 >  5:221205/1‑149 (MQ=255)
                                                                                              tGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACACCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGt                                               <  1:165603/149‑1 (MQ=255)
                                                                                                gAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTg                                              <  5:151751/148‑1 (MQ=255)
                                                                                                gAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGa                                             >  6:169695/1‑149 (MQ=255)
                                                                                                   cTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGt                                          <  6:98878/149‑1 (MQ=255)
                                                                                                        ggATAAAGGTCTTATCTGATGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGAc                                     <  3:280045/149‑1 (MQ=255)
                                                                                                           tAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGcc                                  >  6:371279/1‑149 (MQ=255)
                                                                                                             aaGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCt                                 <  6:221205/148‑1 (MQ=255)
                                                                                                              aGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGc                               >  7:59829/1‑149 (MQ=255)
                                                                                                              aGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGc                               >  2:333649/1‑149 (MQ=255)
                                                                                                              aGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGc                               <  3:403113/149‑1 (MQ=255)
                                                                                                                     aTCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATc                         >  3:288725/1‑148 (MQ=255)
                                                                                                                      tCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCaa                       >  5:432328/1‑149 (MQ=255)
                                                                                                                           ggCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCg                  <  6:432328/149‑1 (MQ=255)
                                                                                                                            gCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACAt                          >  6:385632/1‑140 (MQ=255)
                                                                                                                            gCCTTGCTTGCACACGGGCGCGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACAt                          <  5:385632/140‑1 (MQ=255)
                                                                                                                             ccTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAg                <  5:62427/149‑1 (MQ=255)
                                                                                                                             ccTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAg                <  4:288725/149‑1 (MQ=255)
                                                                                                                              cttgcttgCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAgg               >  5:348168/1‑149 (MQ=255)
                                                                                                                              cttgcttgCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAgg               <  8:207194/149‑1 (MQ=255)
                                                                                                                                   ttgcACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACCTCAATACCGAGGTAAc           >  6:214844/1‑148 (MQ=255)
                                                                                                                                      cacaCGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTTGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGt       <  3:232282/149‑1 (MQ=255)
                                                                                                                                       acacGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGCGGTAACTAGtt      >  4:159407/1‑149 (MQ=255)
                                                                                                                                        cacGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGttt     >  7:259183/1‑149 (MQ=255)
                                                                                                                                         acGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGttt     >  4:401350/1‑148 (MQ=255)
                                                                                                                                           gggcgggAGGTTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGTTTcac  >  5:175632/1‑149 (MQ=255)
                                                                                                                                           gggcgggAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGcc                              <  7:360260/121‑1 (MQ=255)
                                                                                                                                           gggcgggAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGcc                              >  8:360260/1‑121 (MQ=255)
                                                                                                                                           gggcgggAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACAt                          <  5:380224/125‑1 (MQ=255)
                                                                                                                                           gggcgggAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACAt                          >  6:380224/1‑125 (MQ=255)
                                                                                                                                           gggcgggAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGTTTcac  <  3:28952/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
AAGATGCCCAAGGCTCGACATTGCGCCATGACGACTGGAAAATGCAGGTTGTCGAAAATCCACCGATCGCCAAGATCAAGCATCGCGCCCAGCGTGGAGCTGAAGGATAAAGGGCTTATCTGAGGCCTTGCTTGCACACGGGCGGGAACCTCCCGCCCATATGCGCGTACTTCATAGCGCGACAATCCACTCCAAGACCTCACATCTCACTTGGCTGAACTGGGCCTAAAACTCTCCATAGGTGATGTCTGACGCCTGCCACATCAATACCGAGGTAACGAGTTTCAC  >  NC_002947/4488844‑4489131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑