Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 334,837 G→C 7.7% intergenic (‑152/+136) PP_0276 ← / ← PP_t73 Cro/CI family transcriptional regulator/tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947334,8370GC 7.7% 240.3 / 8.8 78intergenic (‑152/+136)PP_0276/PP_t73Cro/CI family transcriptional regulator/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base C (2/4);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

TGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCG  >  NC_002947/334691‑334980
                                                                                                                                                    |                                                                                                                                               
tGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCtgctgc                                                                                                                                                <  3:398459/148‑1 (MQ=255)
    tCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGc                                                                                                                                           >  2:148678/1‑149 (MQ=255)
    tCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGc                                                                                                                                           >  8:177517/1‑149 (MQ=255)
      ggCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTgcg                                                                                                                                               <  5:355273/143‑1 (MQ=255)
      ggCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTgcg                                                                                                                                               <  5:361235/143‑1 (MQ=255)
      ggCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTgcg                                                                                                                                               >  6:355273/1‑143 (MQ=255)
      ggCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTgcg                                                                                                                                               >  6:361235/1‑143 (MQ=255)
      ggCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCGCAGCAGccc                                                                                                                                         <  2:156692/149‑1 (MQ=255)
       gCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCa                                                                                                                                        >  8:130061/1‑149 (MQ=255)
       gCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCGCAGCAGcccc                                                                                                                                        <  8:101971/149‑2 (MQ=255)
        cAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAACGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCGCAGCAGcccca                                                                                                                                       <  8:84449/149‑3 (MQ=255)
         aGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATc                                                                                                                                      >  1:395972/1‑149 (MQ=255)
          gAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGTGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCg                                                                                                                                     <  1:23958/149‑1 (MQ=255)
           aaaTATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGctgctg                                                                                                                                                 <  8:2421/136‑1 (MQ=255)
           aaaTATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGctgctg                                                                                                                                                 >  7:2421/1‑136 (MQ=255)
           aaaTATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGc                                                                                                                                    <  1:260457/149‑1 (MQ=255)
            aaTATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCa                                                                                                                                   <  5:219524/149‑1 (MQ=255)
            aaTATCTAAAATTTGAGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGc                                                                                                                                    <  8:285522/148‑1 (MQ=255)
             atatCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAg                                                                                                                                  <  3:244090/149‑1 (MQ=255)
             atatCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGGCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAg                                                                                                                                  <  4:256004/149‑1 (MQ=255)
                tCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGc                                                                                                                                >  8:367160/1‑148 (MQ=255)
                  tAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAg                                                                                                                             >  4:153470/1‑149 (MQ=255)
                    aaaTTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCaa                                                                                                                              <  2:349462/146‑1 (MQ=255)
                       tttGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGc                                                                                                                        >  5:338625/1‑149 (MQ=255)
                         tGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCt                                                                                                                       <  6:309440/148‑1 (MQ=255)
                          gCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTcc                                                                                                                     >  1:172426/1‑149 (MQ=255)
                          gCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTc                                                                                                                      <  1:209076/148‑1 (MQ=255)
                            gATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCGCAGCAGcccca                                                                                                                                       <  5:185778/129‑3 (MQ=255)
                            gATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCGCAGCAGcccca                                                                                                                                       >  6:185778/1‑127 (MQ=255)
                             aTAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTac                                                                                                                  <  5:173554/149‑1 (MQ=255)
                              tAAATCGTAGTGTGGATTGGTGGGCGTGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GGGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTaca                                                                                                                 <  5:216518/149‑1 (MQ=255)
                                  tCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGa                                                                                                             >  2:63649/1‑149 (MQ=255)
                                  tCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGa                                                                                                             >  4:122735/1‑149 (MQ=255)
                                    gTAGTGTGGATTGGTTGGCGAGTCACAGCGGTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATc                                                                                                           <  8:162228/149‑1 (MQ=255)
                                    gTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGAt                                                                                                            <  3:148162/148‑1 (MQ=255)
                                       gtgtGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtg                                                                                                        <  3:153470/149‑1 (MQ=255)
                                       gtgtGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgt                                                                                                         <  7:367160/148‑1 (MQ=255)
                                        tgtgGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtgg                                                                                                       <  7:205771/149‑1 (MQ=255)
                                          tgGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                      <  2:395972/148‑1 (MQ=255)
                                          tgGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtggt                                                                                                      <  5:16861/148‑1 (MQ=255)
                                             aTTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCaa                                                                                                                              <  2:272619/121‑1 (MQ=255)
                                             aTTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCaa                                                                                                                              >  1:272619/1‑121 (MQ=255)
                                             aTTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCt                                                                                                                    >  4:266828/1‑131 (MQ=255)
                                             aTTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCt                                                                                                                    <  3:266828/131‑1 (MQ=255)
                                             aTTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGAt                                                                                                  >  7:12554/1‑149 (MQ=255)
                                               tggttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGAtt                                                                                                 >  2:225067/1‑148 (MQ=255)
                                                  ttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCgtgg                                                                                                       <  4:408360/139‑1 (MQ=255)
                                                  ttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAgg                                                                                             <  7:177517/149‑1 (MQ=255)
                                                  ttggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCAATCGCAGGCAAGCCAGCTCCTACACCGATCgtgg                                                                                                       >  3:408360/1‑139 (MQ=255)
                                                    ggCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTATGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAggg                                                                                            >  7:225647/1‑148 (MQ=255)
                                                     gCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGc                                                                                           <  6:338625/148‑1 (MQ=255)
                                                      cGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCAt                                                                                         <  3:374582/149‑1 (MQ=255)
                                                       gAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACAACGATCGTGGTGGATTCAGGGCATc                                                                                        >  3:362254/1‑149 (MQ=255)
                                                          tCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCAt                                                                                         >  8:320865/1‑145 (MQ=255)
                                                          tCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCAt                                                                                         <  7:320865/145‑1 (MQ=255)
                                                            aaaGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGt                                                                                     >  7:58866/1‑147 (MQ=255)
                                                             aaGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAg                                                                                  >  5:160757/1‑149 (MQ=255)
                                                              aGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGTG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCATGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCGGTaatg                                                                                 >  1:79374/1‑147 (MQ=255)
                                                                  tttGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAgg                                                                             <  2:21676/149‑1 (MQ=255)
                                                                     gtgtTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATCAAGAGAATTGCTTCTGATTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGAAAGCCAGATCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGa                                                                           >  1:85017/1‑149 (MQ=255)
                                                                     gtgtTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGa                                                                           <  3:122735/148‑1 (MQ=255)
                                                                          ggAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAgg                                                                     >  2:423275/1‑149 (MQ=255)
                                                                          ggAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAg                                                                      <  3:261895/148‑1 (MQ=255)
                                                                                   gtCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCggg                                                             <  8:225647/148‑1 (MQ=255)
                                                                                       gTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGGAGTGTTTGGGGCTGCTGCGCAGCCAATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCgggggg                                                          >  1:89595/1‑148 (MQ=255)
                                                                                           ttGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAAc                                                     <  7:130061/148‑1 (MQ=255)
                                                                                                      tGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCCAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCa                                         >  2:185000/1‑149 (MQ=255)
                                                                                                      tGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCa                                         >  3:292679/1‑149 (MQ=255)
                                                                                                        gTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCAcc                                       >  1:68976/1‑149 (MQ=255)
                                                                                                           aTC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCttt                                    <  1:423275/149‑1 (MQ=255)
                                                                                                            tC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGTAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTc                                   <  1:148678/149‑1 (MQ=255)
                                                                                                               agagAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGAGTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCgg                                 >  6:322429/1‑149 (MQ=255)
                                                                                                                 agaATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTg                               >  7:189244/1‑149 (MQ=255)
                                                                                                                   aaTTGCTTCTGCTTGGT‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAg                             <  2:85017/149‑1 (MQ=255)
                                                                                                                     ttGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGAtt                                                                         <  7:334911/103‑1 (MQ=255)
                                                                                                                     ttGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGAtt                                                                         >  8:334911/1‑103 (MQ=255)
                                                                                                                        cTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCTGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCttt                        >  1:140583/1‑149 (MQ=255)
                                                                                                                         ttCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCtttt                       >  7:411628/1‑149 (MQ=255)
                                                                                                                         ttCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCtttt                       >  1:19027/1‑149 (MQ=255)
                                                                                                                                     ctgcgatgggCTGCGCAGCAGCCCCTCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCg            >  1:277607/8‑149 (MQ=255)
                                                                                                                                       ggtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTa          <  8:58866/149‑1 (MQ=255)
                                                                                                                                        gtTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTAt         >  3:211280/1‑149 (MQ=255)
                                                                                                                                            gggCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGt      <  2:19027/148‑1 (MQ=255)
                                                                                                                                             ggCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGCCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGc    <  1:63649/149‑1 (MQ=255)
                                                                                                                                              gctgctGCGCAGCCCATCGCAGGCTAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGcc   <  4:292679/149‑1 (MQ=255)
                                                                                                                                               ctgctgCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCg  >  6:115635/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGCCTCGGCAGAAATATCTAAAATTTGCGATAAATCGTAGTGTGGATTGGTTGGCGAGTCAAAGCGTTTGTGTTGGAGAGTGTGTCTGTTTTTGAGCTGTGCTGGTGATC‑AGAGAATTGCTTCTGCTTGGG‑GTGGTTGGGGCTGCTGCGCAGCCCATCGCAGGCAAGCCAGCTCCTACACCGATCGTGGTGGATTCAGGGCATCCGTAAGGCAGGGATTGAGGGCTGCGGGGGGAAAACAAAAAAGGCCCACCTTTCGGTGAGCCTTTTTTGATACTGCGTATGGTGCCG  >  NC_002947/334691‑334980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑