Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,516,192	C→G	6.1%	Y19* (TAC→TAG)	ate →	arginyl‑tRNA‑‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,516,192	0	C	G	6.1%	207.6 / 4.2	66	Y19* (TAC→TAG)	ate	arginyl‑tRNA‑‑protein transferase
Reads supporting (aligned to +/- strand):  ref base C (29/32);  new base G (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

CTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGCGTGC  >  NC_002947/4516044‑4516340                                                                                                                                                     |                                                                                                                                                     cTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAc                                                                                                                                                      >  7:51083/1‑149 (MQ=255) cTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTAc                                                                                                                                                      >  4:308111/1‑149 (MQ=255)    gACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTg                                                                                                                                                   >  4:94093/1‑149 (MQ=255)      ccAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGcc                                                                                                                                                 <  2:404332/149‑1 (MQ=255)       cAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCg                                                                                                                                                >  6:256632/1‑149 (MQ=255)          cccAACCGCGCGTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTAAAAAAAAAGTCTCACCGAAGGTGTTGATCATGACAGAGTTGGCGCGGTTTAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                             <  8:343343/149‑1 (MQ=255)          cccAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                             <  2:418370/149‑1 (MQ=255)          cccAACAGCGCATCATTGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGTGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggag                                                                                                                                             <  5:221146/149‑1 (MQ=255)            cAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggagga                                                                                                                                           <  2:101041/149‑1 (MQ=255)             aaCAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAATAAAAAGTCTCACCGAAGTGGTTGATAATGACAGAGTTGGCGCGGTTGAAGTTCTATGGCACTCAGCCCCACTCCTGCAGCTACCTGCCGgaggag                                                                                                                                          >  2:41414/1‑149 (MQ=255)              aCAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCGGCAGGTAGCTGCAggagtggg                                                                                                                                         <  3:325814/149‑5 (MQ=255)                aGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAg                                                                                                                                       >  8:232541/1‑149 (MQ=255)                  cgcTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGc                                                                                                                                     <  8:51083/149‑1 (MQ=255)                  cgcTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGc                                                                                                                                     <  6:275215/149‑1 (MQ=255)                       cATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGAcc                                                                                                                                 <  7:183992/148‑1 (MQ=255)                            gTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTg                                                                                                                           >  4:207565/1‑149 (MQ=255)                            gTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTg                                                                                                                           <  2:383739/149‑1 (MQ=255)                                ccTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTcc                                                                                                                       <  7:232541/149‑1 (MQ=255)                                ccTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTc                                                                                                                        <  3:306951/148‑1 (MQ=255)                                  tAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTc                                                                                                                     >  6:170407/1‑149 (MQ=255)                                        aGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGAccc                                                                                                                >  4:216024/1‑148 (MQ=255)                                                   cTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGAt                                                                                                    <  5:359972/149‑1 (MQ=255)                                                      gCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGa                                                                                                 <  5:256632/149‑1 (MQ=255)                                                        ttACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACg                                                                                               >  7:119048/1‑149 (MQ=255)                                                            acTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCATGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCa                                                                                           >  1:194487/1‑149 (MQ=255)                                                             cTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCAc                                                                                          >  7:237889/1‑149 (MQ=255)                                                              ttAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCCGCCAGCCGATGGACGTGCACg                                                                                         >  4:248557/1‑149 (MQ=255)                                                              ttAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                         >  5:411369/1‑149 (MQ=255)                                                                aaaaaaaaGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACgt                                                                                        <  6:379643/148‑1 (MQ=255)                                                                 aaaaaaaGACTCCCCGAAGGTGTTGATCAGGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACgt                                                                                        <  4:359953/147‑1 (MQ=255)                                                                       aGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCg                                                                                <  3:216024/149‑1 (MQ=255)                                                                           tcACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGGCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCATCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGAttt                                                                            >  4:247757/1‑149 (MQ=255)                                                                             aCCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGt                                                                          >  1:131654/1‑149 (MQ=255)                                                                              ccGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTc                                                                         <  7:80314/149‑1 (MQ=255)                                                                              ccGAAGGGGATGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTc                                                                         <  7:380210/149‑1 (MQ=255)                                                                               cGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCAGATTTGTCg                                                                        >  4:124453/1‑149 (MQ=255)                                                                               cGAAGCGGTTGATCATGACAGAGTTGGCGCGGGTGAAGTGCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                        <  4:100392/149‑1 (MQ=255)                                                                                       ttGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATggg                                                                <  8:237889/149‑1 (MQ=255)                                                                                         taTCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGc                                                               <  5:355358/147‑1 (MQ=255)                                                                                          aTCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCt                                                              <  8:119048/148‑1 (MQ=255)                                                                                          aTCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCt                                                              <  6:411369/148‑1 (MQ=255)                                                                                             aTGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTc                                                           <  3:308111/148‑1 (MQ=255)                                                                                                           gcgGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGac                                             >  3:245165/1‑148 (MQ=255)                                                                                                           gcgGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTCCCGGAGGAGCACGCGACCACGCTGATACTCGACCCCAGCCAGCCGATCGACGTGCACGTCTACGCCCATATGTCGTAAATGGGCTTTCGACGCAgatgcgcc                                             >  5:414532/1‑141 (MQ=255)                                                                                                               ttGAAGTTCTATGCCACTCAGCCCCACACCTGCAGCTACCTGCCTGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCACCCAGCCGATGGACGTGCACGTGAACGCCGATTTGTTCGAAATGGGCTTTCGCCGCAGTGGCGaccac                                          <  1:41414/147‑1 (MQ=255)                                                                                                                   aGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTAc                                    <  5:170407/149‑1 (MQ=255)                                                                                                                     ttCTATGCCACTCAGTCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGATCACCTGTACCg                                  >  4:130816/1‑149 (MQ=255)                                                                                                                       cTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGcc                                >  4:209286/1‑149 (MQ=255)                                                                                                                       cTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGc                                 <  3:248557/148‑1 (MQ=255)                                                                                                                         aTGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGAGGCGACCACCTGTACCGCCca                              >  4:226531/1‑149 (MQ=255)                                                                                                                            ccACTCACCCCCACTCATGCAGCTAACTTCCGTCGGAGCAGGCTACCACGCTGTTCATCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCTCAGTGGCGACCACCTGTACCGCCCacac                           <  3:51749/149‑1 (MQ=255)                                                                                                                              aCTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGCCCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                         >  5:37440/1‑149 (MQ=255)                                                                                                                              aCTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                                 <  2:416211/109‑1 (MQ=255)                                                                                                                              aCTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                                 >  1:416211/1‑109 (MQ=255)                                                                                                                                  aGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGcc                       <  3:226531/147‑1 (MQ=255)                                                                                                                                   ctgctccTCCGGCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAg                     >  7:187311/7‑148 (MQ=255)                                                                                                                                    ccccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGaa                   >  1:410255/1‑149 (MQ=255)                                                                                                                                    ccccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGaa                   >  7:180616/1‑149 (MQ=255)                                                                                                                                     cccACTCCTGCAGCTACCTGCGGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAAc                  >  5:271470/1‑149 (MQ=255)                                                                                                                                        aCTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTg                >  6:45602/1‑148 (MQ=255)                                                                                                                                        aCTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTg                <  5:45602/148‑1 (MQ=255)                                                                                                                                           ccggCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGcc                                <  3:234085/126‑1 (MQ=255)                                                                                                                                           ccggCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGcc                                >  4:234085/4‑129 (MQ=255)                                                                                                                                            cTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAAc            <  2:131654/148‑1 (MQ=255)                                                                                                                                            cTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACg           >  6:27864/1‑149 (MQ=255)                                                                                                                                            cTGCAGCTACCTGCCGGAGGAGAAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTCTACCCCGAGTTGGCGGAAATGGGCGATCGCCGCAGTGGCGACCACCACTACCGCCCACACTGCCAGAACTGCAACg           >  7:66501/1‑149 (MQ=255)                                                                                                                                             tGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGc          >  4:177706/1‑149 (MQ=255)                                                                                                                                                 gCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGc      <  3:94093/149‑1 (MQ=255)                                                                                                                                                     ccTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGCGTGc  <  5:27864/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CTTGACCAGCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGCGTGC  >  NC_002947/4516044‑4516340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑