Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,614,808	C→A	7.8%	D393E (GAC→GAA)	PP_4085 →	rhs family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,614,808	0	C	A	7.8%	189.0 / 4.3	62	D393E (GAC→GAA)	PP_4085	rhs family protein
Reads supporting (aligned to +/- strand):  ref base C (30/27);  new base A (2/3);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGGCGCCT  >  NC_002947/4614662‑4614954                                                                                                                                                   |                                                                                                                                                   gAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACg                                                                                                                                                   <  4:131824/148‑1 (MQ=255) gAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACg                                                                                                                                                   <  7:222609/148‑1 (MQ=255) gAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACg                                                                                                                                                   >  3:131824/1‑148 (MQ=255)  aGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCTGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGc                                                                                                                                                 <  6:363232/149‑1 (MQ=255)   gCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGCCTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCACTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGc                                                                                                                                                 >  7:279111/1‑148 (MQ=255)    cTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCa                                                                                                                                               <  1:425497/149‑1 (MQ=255)               cccACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGAc                                                                                                                                            >  5:392247/1‑141 (MQ=255)               cccACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGAc                                                                                                                                            <  6:392247/141‑1 (MQ=255)                 cacaTCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCg                                                                                                                                    >  6:111934/1‑147 (MQ=255)                  acaTCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTCCCGGTGATGCCTTCATCAGTGCCGTTGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAg                                                                                                                                 <  3:391720/149‑1 (MQ=255)                           aGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAAt                                                                                                                         >  2:264400/1‑148 (MQ=255)                              cGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACgg                                                                                                                                                  >  8:363774/1‑120 (MQ=255)                              cGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACgg                                                                                                                                                  <  7:363774/120‑1 (MQ=255)                                gTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAggg                                                                                                                   <  4:50254/149‑1 (MQ=255)                                gTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAggg                                                                                                                   <  2:162113/149‑1 (MQ=255)                                      tgtgGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTAt                                                                                                             >  4:198939/1‑149 (MQ=255)                                       gtgGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATc                                                                                                            >  8:298247/1‑149 (MQ=255)                                        tgGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATc                                                                                                            <  7:167833/148‑1 (MQ=255)                                              tACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCtt                                                                                                     >  3:195631/1‑149 (MQ=255)                                                 aTCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGa                                                                                                  >  5:48333/1‑149 (MQ=255)                                                   cGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATa                                                                                                >  7:142933/1‑149 (MQ=255)                                                        acgacgGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGAc                                                                                           <  7:298247/149‑1 (MQ=255)                                                        acgacgGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGAc                                                                                           >  8:56936/1‑149 (MQ=255)                                                               tAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGa                                                                                    <  5:93019/149‑1 (MQ=255)                                                                 aCCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAAt                                                                                  >  7:383212/1‑149 (MQ=255)                                                                  ccGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATc                                                                                 <  1:242423/149‑1 (MQ=255)                                                                   cGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGTACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATcc                                                                                <  3:248629/149‑1 (MQ=255)                                                                   cGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATcc                                                                                >  3:277099/1‑149 (MQ=255)                                                                        ggAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGtt                                                                                                                                        <  1:384096/88‑1 (MQ=255)                                                                        ggAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGtt                                                                                                                                        >  2:384096/1‑88 (MQ=255)                                                                         gAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACg                                                                          >  5:3302/1‑149 (MQ=255)                                                                                gccgccTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACgg                                                                   <  1:231730/149‑1 (MQ=255)                                                                                gccgccTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACgg                                                                   >  1:304188/1‑149 (MQ=255)                                                                                gccgccTCAGTGCCGGTGATGCCTCCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACgg                                                                   >  8:37416/1‑149 (MQ=255)                                                                                      tCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGAt                                                              >  2:142350/1‑148 (MQ=255)                                                                                      tCAGTGCCGGAGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGAt                                                              >  8:139612/1‑148 (MQ=255)                                                                                             cGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCg                                                      <  1:264400/149‑1 (MQ=255)                                                                                                   tGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGAcc                                                <  7:56936/149‑1 (MQ=255)                                                                                                     ccTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCg                                               <  8:142933/148‑1 (MQ=255)                                                                                                        tcatcaGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGCCGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGCCGGCact                                           >  8:324787/1‑147 (MQ=255)                                                                                                            caGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAAtctc                                        >  3:397727/1‑148 (MQ=255)                                                                                                            caGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAAtctc                                        >  8:60894/1‑148 (MQ=255)                                                                                                            caGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAAactc                                        >  7:118562/1‑148 (MQ=255)                                                                                                              gTGCCGTGGACGGCGTGGCCTTCTTCCAGCTCGAGGACGGCCAGCCCGTTGTCGAAGATATCAATGAAGGGCTTTATCGCTTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACCGCTGATCAGGCTCGGCGACCGCAATCTCAAt                                     <  8:279111/149‑1 (MQ=255)                                                                                                                       aCGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGAc                            <  8:383212/149‑1 (MQ=255)                                                                                                                        cggtctGGCCGTCCTCCAGCTGGAAGAAGGCCAAGCCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTc                               <  6:156001/140‑1 (MQ=38)                                                                                                                        cggtctGGCCGTCCTCCAGCTGGAAGAAGGCCAAGCCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTc                               >  5:156001/6‑145 (MQ=38)                                                                                                                           tctGGCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgc                        <  4:65895/147‑1 (MQ=255)                                                                                                                           tatGGCCGTCCTCCAGCTGGAAGAAGGCCAGACCGTTGTCTAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgc                        >  8:250617/3‑149 (MQ=255)                                                                                                                           cTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTctccatgc                        >  4:307958/1‑142 (MQ=255)                                                                                                                            ttGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCg                                                                                                   <  3:118642/73‑1 (MQ=255)                                                                                                                            ttGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCg                                                                                                   >  4:118642/1‑73 (MQ=255)                                                                                                                              ggCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACctgctg                      <  3:198939/148‑1 (MQ=255)                                                                                                                               gCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATAAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGt                     <  8:118562/148‑1 (MQ=255)                                                                                                                                    cttcCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGTGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTacgac                <  5:111934/148‑1 (MQ=255)                                                                                                                                    cttcCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTacga                 <  4:397727/147‑1 (MQ=255)                                                                                                                                       ccAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGaa             >  7:373948/1‑148 (MQ=255)                                                                                                                                        cAGCTGGAAGAAGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACa           <  7:250617/149‑1 (MQ=255)                                                                                                                                         aGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGATAGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTacgactccaaa          >  8:349600/1‑143 (MQ=255)                                                                                                                                              gaggaCGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGgcg     >  1:427382/1‑149 (MQ=255)                                                                                                                                               aggaCGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGgcgc    <  2:427382/149‑1 (MQ=255)                                                                                                                                                 gaCGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGGCGCCt  >  8:104541/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GAGCTGGCCCGCGTCCCACATCCTCAAGGCGGTACGCTGTGGATCTACATCGACAACGACGGTAACCGACTGGAACTGGGCCGCCTCAGTGCCGGTGATGCCTTCATCAGTGCCGTGGACGGCTTGGCCTTCTTCCAGCTGGAGGACGGCCAGACCGTTGTCGAAGATATCAATGAAGGGCTTTATCGGGTCTTCGATACCGACCCGCTGAATCCGAAACGCTCACGGCTGATCAGGCTCGGCGACCGCAATCTCAATTGCCTCGACCTGCTGTACGACGAACAAGGGCGCCT  >  NC_002947/4614662‑4614954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑