Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,673,551 G→C 8.0% G108A (GGG→GCG) ‡ PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,673,5510GC 8.0% 195.7 / 5.9 62G108A (GGG→GCG) ‡PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (2/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGGCC  >  NC_002947/4673403‑4673699
                                                                                                                                                    |                                                                                                                                                     
aaCCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCggg                                                                                                                                                       <  4:85105/149‑1 (MQ=255)
 aCCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCgggg                                                                                                                                                      <  8:123192/149‑1 (MQ=255)
      ggggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCCGGACGGGCTGGCCTACCAGCCAGGGGTGTTCGCCCAAGCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGTCCTCAACCGTGCGCCGGGGGCGCa                                                                                                                                                 <  4:103164/147‑1 (MQ=255)
       tggACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTGCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑c                                                                                                                                               <  5:136373/149‑1 (MQ=255)
         gACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑Cgg                                                                                                                                             >  4:86425/1‑149 (MQ=255)
         gACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑Cgg                                                                                                                                             >  3:401890/1‑149 (MQ=255)
           cATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCA‑CGGtt                                                                                                                                           <  6:188326/149‑1 (MQ=255)
            aTGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTc                                                                                                                                          <  1:45523/149‑1 (MQ=255)
            aTGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCA‑CGGtt                                                                                                                                           <  6:248772/148‑1 (MQ=255)
            aTGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCA‑CGGtt                                                                                                                                           >  5:248772/1‑148 (MQ=255)
              gCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCA‑CGGtt                                                                                                                                           <  8:83041/146‑1 (MQ=255)
              gCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCCCCGGCGCA‑CGGtt                                                                                                                                           >  7:83041/1‑146 (MQ=255)
               cAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCgg                                                                                                                                       >  6:375451/1‑149 (MQ=255)
               cAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCgg                                                                                                                                       >  1:245331/1‑149 (MQ=255)
               cAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCg                                                                                                                                        <  4:395540/148‑1 (MQ=255)
                 gcgcGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGgtg                                                                                                                                     >  7:31545/1‑149 (MQ=255)
                    cgTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTc                                                                                                                                   >  6:26040/1‑148 (MQ=255)
                                 cGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGa                                                                                                                     <  3:186666/149‑1 (MQ=255)
                                         cAGCAACCAGGACGTGCTGGCCTAACAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCGCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGc                                                                                                             >  6:110444/1‑149 (MQ=255)
                                           gCAACCAGGACGTGCTGGCCTACCAGCCAGGGGGGTGCGCCCAATCGGCAGGTAACGATGGCAGCAAGCTGTCTATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCg                                                                                                           <  1:372312/149‑1 (MQ=255)
                                              aCCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTg                                                                                                        <  5:375451/149‑1 (MQ=255)
                                                cAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGAc                                                                                                      >  5:81135/1‑149 (MQ=255)
                                                    aCGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCGCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGc                                                                                                  <  5:413270/149‑1 (MQ=255)
                                                      gTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGccc                                                                                                >  6:328353/1‑149 (MQ=255)
                                                         cTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCgg                                                                                              <  3:86425/148‑1 (MQ=255)
                                                            gCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGggcgg                                                                                          >  7:364283/1‑149 (MQ=255)
                                                                 ccagccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                     >  5:244218/1‑149 (MQ=255)
                                                                 ccagccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                     >  3:368391/1‑149 (MQ=255)
                                                                 ccacccagGGGGGTTCGCCCAATCGGCAGGAAACGATGGCATCAACCTGGCGATACGCGGCCCGGGCCTCAACCGGGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGc                                                                                     <  5:255932/149‑1 (MQ=255)
                                                                   agccagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCTCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCt                                                                                   <  7:282326/149‑1 (MQ=255)
                                                                      cagGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTAc                                                                                <  2:245331/149‑1 (MQ=255)
                                                                      cagGGGGGTTCGCCCAATCGGCTGGAAACGAGGGCATCAACCTGTCTATACGCGGCGCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTAc                                                                                <  4:401890/149‑1 (MQ=255)
                                                                       agGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTAc                                                                                >  3:178187/1‑148 (MQ=255)
                                                                              ttCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGCACTGtt                                                                        >  1:420034/1‑149 (MQ=255)
                                                                               tCGCCAAATGGGCAGGTAACGTTGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCGGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGtt                                                                        <  6:244218/148‑1 (MQ=255)
                                                                                cGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTc                                                                       >  7:190961/1‑148 (MQ=255)
                                                                                   ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACGGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAc                                                                   <  4:189233/149‑1 (MQ=255)
                                                                                   ccAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAAc                                                                   >  3:189233/1‑149 (MQ=255)
                                                                                         ggCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCtgt                                                             <  5:328353/149‑1 (MQ=255)
                                                                                           cAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGGGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTgg                                                           <  6:153136/149‑1 (MQ=255)
                                                                                            atgTACCGATGGCATAAAGCTGTCGATACGCGGCGCGGGCCTCAACCGGGGGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGc                                                          <  5:390293/147‑1 (MQ=255)
                                                                                              gTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCt                                                         >  1:288722/1‑148 (MQ=255)
                                                                                               tAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCt                                                         >  5:405119/1‑147 (MQ=255)
                                                                                                aaCGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCCACGGCCTGCCGTTGACCGGCCCGGGCGGCAAGCCTATCGAACTGTTCGAACCGCTGTGGCTGAg                                                      >  7:41407/1‑149 (MQ=255)
                                                                                                  cGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGcc                                                    <  4:368391/149‑1 (MQ=255)
                                                                                                    aTGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGt                                                  <  4:178187/149‑1 (MQ=255)
                                                                                                             aGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGtg                                         <  6:81135/149‑1 (MQ=255)
                                                                                                               cTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGtgct                                       >  7:4746/1‑149 (MQ=255)
                                                                                                                 gTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGtgctgc                                     >  7:235232/1‑149 (MQ=255)
                                                                                                                 gTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGtgctgc                                     >  2:284573/1‑149 (MQ=255)
                                                                                                                     aTACGCGGCTCGGGCCTCAACCGTGGGCCGGGGGCTCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTgg                                 <  5:760/149‑1 (MQ=255)
                                                                                                                      tACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAATCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgc                                <  1:284573/149‑1 (MQ=255)
                                                                                                                      tACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGgc                                >  7:150805/1‑149 (MQ=255)
                                                                                                                        cgggggccGGGCCTCCACCGGGGCCCGGGGGCGCACCGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTgg                                 <  2:420034/140‑1 (MQ=255)
                                                                                                                            gcgcGGGCCGCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGGCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAAt                          <  5:228998/146‑1 (MQ=255)
                                                                                                                                  gCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                    >  3:419628/1‑149 (MQ=255)
                                                                                                                                   ccTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCttt                    >  4:38015/1‑148 (MQ=255)
                                                                                                                                   ccTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTg                   <  8:150805/149‑1 (MQ=255)
                                                                                                                                    cTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGa                  <  2:287193/149‑1 (MQ=255)
                                                                                                                                    cTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGa                  >  4:231504/1‑149 (MQ=255)
                                                                                                                                      cAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGAc                 >  5:116662/1‑148 (MQ=255)
                                                                                                                                        aCCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCaa              <  8:364283/149‑1 (MQ=255)
                                                                                                                                            tGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCt          >  2:153368/1‑149 (MQ=255)
                                                                                                                                              cgcCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTcc        >  3:414070/1‑149 (MQ=255)
                                                                                                                                                    gggCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGGcc  <  8:4746/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACCTGGTGGACATGCAGCGCGTGGGGCAAGGCCGAGTGGCCAGCAACCAGGACGTGCTGGCCTACCAGCCAGGGGTGTTCGCCCAATCGGCAGGTAACGATGGCATCAAGCTGTCGATACGCGGCTCGGGCCTCAACCGTGCGCCGGGGGCGCA‑CGGTTCCGGTGTCTACACCATGTTCGACGGCCTGCCGTTGACCGGCCCGGGCGGCACGCCTTACGAACTGTTCGAACCGCTGTGGCTGAGCCGTGCCGAAGTGCTGCGTGGCGCCAATGGCTTTGACCAAGGCTCCCTGGCC  >  NC_002947/4673403‑4673699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑