Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,694,904	C→T	6.9%	L927L (CTG→TTG)	PP_4154 →	class 3 aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,694,904	0	C	T	6.9%	211.5 / 3.4	72	L927L (CTG→TTG)	PP_4154	class 3 aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (30/37);  new base T (3/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCGCTACTTCAAGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTGGGCGAAGGTCT  >  NC_002947/4694759‑4695052                                                                                                                                                    |                                                                                                                                                     cGGGCGCTACTTCAAGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCtgt                                                                                                                                                   <  2:259188/149‑1 (MQ=255)    tagcTACTTCAAGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCg                                                                                                                                                <  3:5626/147‑1 (MQ=255)        tACTTCAAGGCCCGCCTGCAGGCGCGGGTCGACAATC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGa                                                                                                                                               <  2:248768/146‑1 (MQ=255)        tACTTCAAGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCg                                                                                                                                            >  2:269374/1‑149 (MQ=255)        tACTTCAAGGCACGCCTGCAGGCGCTGGNCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGa                                                                                                                                               >  1:248768/1‑146 (MQ=255)           ttCAAGGCACGCCTGCAGGCGCTGGTCGACAAGCGACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGcc                                                                                                                                          >  1:59321/1‑149 (MQ=255)             cAAGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTg                                                                                                                                        <  7:246112/148‑1 (MQ=255)              aaGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGc                                                                                                                                       <  3:8858/148‑1 (MQ=255)              aaGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGc                                                                                                                                       <  1:269374/148‑1 (MQ=255)                  cACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGTAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGAc                                                                                                                                  <  1:352313/149‑1 (MQ=255)                    cGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCt                                                                                                                                >  2:64793/1‑149 (MQ=255)                     gCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTg                                                                                                                               >  5:275918/1‑149 (MQ=255)                       cgtcAGGCGCTGGTCGACAACC‑ACCCGCTGGCCGGCGCGGCACACTTAAATGGGCTTCTACCTGTGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTgg                                                                                                                              <  1:364772/146‑1 (MQ=255)                          cAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCAt                                                                                                                          >  5:72939/1‑149 (MQ=255)                                   gTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATg                                                                                                                   <  8:385824/147‑1 (MQ=255)                                     cGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGc                                                                                                               >  4:429952/1‑149 (MQ=255)                                     cGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTAACTGGGTCTGGAACTGGTGCGCGCCCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGTTGTGCGATCGCCTGCGTGACCTGGTCATCTTCATGCAGc                                                                                                               >  4:152604/1‑149 (MQ=255)                                     cGAAAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGTCTAGGGATTCTAACTGGGTCTGGAACTGGTGAGCGAACGCACCACACTGGAGCCAGCCAACGAAGAAACCATGATGTTTTGCGATCGCCTGAGTGAAATGGTAATCTTCATTCAg                                                                                                                >  4:158945/1‑148 (MQ=255)                                      gACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCCCCGAAGAAACCATGATGCTGTGCGATCGCCTCCGTGACCTGGGCATCTTCATGCAGcc                                                                                                              >  7:177917/1‑149 (MQ=255)                                        cAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGa                                                                                                            >  7:40734/1‑149 (MQ=255)                                         aaGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGAc                                                                                                           >  8:134091/1‑149 (MQ=255)                                         aaGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGCTCTGCAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATCATGCTGTGCGATCGCCTGCGTGACCTGGGCAACATCATGCAGCCGAc                                                                                                           >  8:316596/1‑149 (MQ=255)                                          aGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGAcc                                                                                                          <  1:2192/149‑1 (MQ=255)                                                ccGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGa                                                                                                     <  2:127238/149‑1 (MQ=255)                                                       ccGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCt                                                                                               >  7:261595/1‑148 (MQ=255)                                                         ggCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGaa                                                                                            <  7:323105/149‑1 (MQ=255)                                                          gcgcGGCACA‑TGGCTCGTGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAAc                                                                                           <  5:128461/149‑1 (MQ=255)                                                            gcgGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACAt                                                                                         >  4:96889/1‑149 (MQ=255)                                                                 aca‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGa                                                                                             >  6:207670/1‑140 (MQ=255)                                                                 aca‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGa                                                                                             <  5:207670/140‑1 (MQ=255)                                                                   a‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAg                                                                                  <  3:234091/149‑1 (MQ=255)                                                                     tGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTc                                                                                     >  1:161013/1‑145 (MQ=255)                                                                     tGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGa                                                                                 <  6:72939/149‑1 (MQ=255)                                                                       gCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGAt                                                                                <  8:61116/148‑1 (MQ=255)                                                                         tCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATc                                                                               >  5:114565/1‑147 (MQ=255)                                                                           gggCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAg                                                                            <  3:339514/148‑1 (MQ=255)                                                                                    ccTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGa                                                                     <  2:16195/146‑1 (MQ=255)                                                                                    ccTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGAt                                                                    <  2:59321/147‑1 (MQ=255)                                                                                    ccTGGGGCTGGAACTGGTGCGCGACCGCACCACCCGGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGa                                                                     <  4:115350/146‑1 (MQ=255)                                                                                          tCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTg                                                                                              >  2:175524/1‑115 (MQ=255)                                                                                          tCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCAc                                                              >  2:181694/1‑147 (MQ=255)                                                                                             ggAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGcc                                                         >  1:57586/1‑149 (MQ=255)                                                                                              gAACTGGTTCGCGACCGCACCACCCTGGAGCCGTCCACCGAAGAAACGATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGc                                                          <  4:316423/147‑1 (MQ=255)                                                                                               aaCTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGt                                                       <  8:40734/149‑1 (MQ=255)                                                                                                    gTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGCCCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCATCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAg                                                  >  1:266231/1‑149 (MQ=255)                                                                                                    gTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGc                                                                <  7:116671/135‑1 (MQ=255)                                                                                                    gTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGc                                                                >  8:116671/1‑135 (MQ=255)                                                                                                    gTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCg                                                    <  6:275918/147‑1 (MQ=255)                                                                                                    gTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGa                                                   <  6:204752/148‑1 (MQ=255)                                                                                                     tGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGc                                                 >  2:167476/1‑149 (MQ=255)                                                                                                      gcgcGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGTTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCg                                                >  8:56843/1‑149 (MQ=255)                                                                                                       cgcgACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGt                                               >  3:88517/1‑149 (MQ=255)                                                                                                        gcgACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTg                                              <  3:352791/149‑1 (MQ=255)                                                                                                        gcgACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTg                                              <  6:114565/149‑1 (MQ=255)                                                                                                        gcgACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGAGAGCGTg                                              >  4:76713/1‑149 (MQ=255)                                                                                                           aCCGCACCACCCTGGAGCCGGCCACCGATGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGac                                           <  2:57586/149‑1 (MQ=255)                                                                                                             cGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGacta                                         >  3:60234/1‑149 (MQ=255)                                                                                                               caccacCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGactact                                       <  1:143379/149‑1 (MQ=255)                                                                                                                accaccCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGa                                            >  2:215537/1‑143 (MQ=255)                                                                                                                accaccCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGa                                            <  1:215537/143‑1 (MQ=255)                                                                                                                    cccTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTc                                  <  4:60234/149‑1 (MQ=255)                                                                                                                      cTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGa                                <  3:76713/149‑1 (MQ=255)                                                                                                                             cGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCAtt                         <  2:161013/149‑1 (MQ=255)                                                                                                                                      aagaaACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCAGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTg                >  4:220168/1‑149 (MQ=255)                                                                                                                                         aaaCCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTg             >  5:241498/1‑149 (MQ=255)                                                                                                                                          aaCCATGATCCGCTGCGATCGCCTGCGGGACCTGGGCATCTTCATGCAGCCGACCGGCTATTACCTGAACATCCTCAAGAGCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGAGGACTACTTTGTCGACAGCATTGACCGGGTGCTgg            <  1:64793/149‑1 (MQ=255)                                                                                                                                           aCCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTggg           >  2:394399/1‑149 (MQ=255)                                                                                                                                           aCCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTggg           <  3:96889/149‑1 (MQ=255)                                                                                                                                             cATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCACCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTGGGc          >  1:310245/1‑148 (MQ=255)                                                                                                                                                 atgTTGTGCGATCGCCTGCGTGACCTTGGCATCTTCTTGCAGCCTACCTGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGAGAGCATTGACCGTTTGCTGGGCTAAg      <  3:158945/148‑1 (MQ=255)                                                                                                                                                 atgTTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGAACGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTGGGCGAAg      <  3:152604/148‑1 (MQ=255)                                                                                                                                                 atgCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTGGGCGAAg      <  1:394399/148‑1 (MQ=255)                                                                                                                                                    cTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTGGGCGAAGGTCt  >  7:172561/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGGGCGCTACTTCAAGGCACGCCTGCAGGCGCTGGTCGACAAGC‑ACCCGCTGGCCGGCGCGGCACA‑TGGCTCGGGCTTCTACCTGGGTCTGGAACTGGTGCGCGACCGCACCACCCTGGAGCCGGCCACCGAAGAAACCATGATGCTGTGCGATCGCCTGCGTGACCTGGGCATCTTCATGCAGCCGACCGGCGATTACCTGAACATCCTCAAGATCAAGCCGCCGATGTGCACCAGCCGTGCGAGCGTGGACTACTTTGTCGACAGCATTGACCGGGTGCTGGGCGAAGGTCT  >  NC_002947/4694759‑4695052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑