Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,711,817	C→G	6.3%	L17V (CTG→GTG) ‡	PP_4171 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,711,817	0	C	G	6.3%	249.2 / 6.0	79	L17V (CTG→GTG) ‡	PP_4171	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base G (3/2);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

CGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCG  >  NC_002947/4711669‑4711964                                                                                                                                                     |                                                                                                                                                     cGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGccc                                                                                                                                                      >  5:393854/1‑149 (MQ=255)  gCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCt                                                                                                                                                     <  3:347427/149‑1 (MQ=255)           tACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggc                                                                                                                                              >  6:202520/1‑147 (MQ=255)           tACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggc                                                                                                                                              <  5:202520/147‑1 (MQ=255)              ttttCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCa                                                                                                                                         <  8:22998/149‑1 (MQ=255)                    aGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTc                                                                                                                                          >  6:292882/1‑142 (MQ=255)                    aGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTc                                                                                                                                          <  5:292882/142‑1 (MQ=255)                     gACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTg                                                                                                                                  >  1:346933/1‑149 (MQ=255)                              ccGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCgacg‑ac                                                                                                                        >  4:196792/1‑149 (MQ=255)                                 acacCCACTAGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTg                                                                                                                     >  6:298386/1‑149 (MQ=255)                                   acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGTCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggcgg                                                                                                                                            >  6:139708/1‑125 (MQ=255)                                   acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGTCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggcgg                                                                                                                                            <  5:139708/125‑1 (MQ=255)                                   acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGt                                                                                                                    >  4:369245/1‑148 (MQ=255)                                     ccACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCa                                                                                                                 <  6:59192/149‑1 (MQ=255)                                      cACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAg                                                                                                                                        >  2:298371/1‑126 (MQ=255)                                      cACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCAt                                                                                                                <  2:207954/149‑1 (MQ=255)                                         tGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTCa                                                                                                                                         >  4:247331/1‑122 (MQ=255)                                         tGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTCa                                                                                                                                         <  3:247331/122‑1 (MQ=255)                                             aaaaCCCCCACGGTGAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaac                                                                                                         <  2:7969/149‑1 (MQ=255)                                             aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                          >  4:58028/1‑148 (MQ=255)                                             aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                          >  5:203107/1‑148 (MQ=255)                                             aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                          >  8:99689/1‑148 (MQ=255)                                                acacccACGGTCATTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCTGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACAcc                                                                                                      <  6:241190/146‑1 (MQ=255)                                                 cccccACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACAcc                                                                                                      >  1:123133/1‑148 (MQ=255)                                                   cccACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGAc                                                                                                   <  5:196908/149‑1 (MQ=255)                                                         gTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCca                                                                                             <  3:195609/149‑1 (MQ=255)                                                              ttACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGt                                                                                        >  5:166227/1‑149 (MQ=255)                                                                aCCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGtt                                                                                       <  3:196792/148‑1 (MQ=255)                                                                 ccATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTc                                                                                      <  2:289500/148‑1 (MQ=255)                                                                   aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                   <  6:393854/149‑1 (MQ=255)                                                                   aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                   >  8:69802/1‑149 (MQ=255)                                                                   aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCGGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                   >  2:254599/1‑149 (MQ=255)                                                                      ccTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCg                                                                                <  5:298386/149‑1 (MQ=255)                                                                        tGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                               >  6:436523/1‑148 (MQ=255)                                                                           ttCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑CCCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCcaca                                                                           >  2:278563/1‑149 (MQ=255)                                                                           ttCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCcaca                                                                           >  1:427570/1‑149 (MQ=255)                                                                             cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                               <  8:361292/143‑1 (MQ=255)                                                                             cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                               >  7:361292/1‑143 (MQ=255)                                                                             cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCAACGACAACACCGACCTGCCACAGGTTCTGACCGCCACACg                                                                         >  1:200013/1‑149 (MQ=255)                                                                              aaTCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCacaccc                                                                        <  7:99689/149‑3 (MQ=255)                                                                                tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGGGGTCAGGCCGTGGCCGACGAAGAAGTTAATCCACAACACCGACAGGCCACAGGTTATGCCCCCCCCACGcc                                                                       >  1:142466/1‑149 (MQ=255)                                                                                tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCt                                                                      >  8:75864/1‑149 (MQ=255)                                                                                tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCt                                                                      >  4:214831/1‑149 (MQ=255)                                                                                  cTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTg                                                                     <  5:169055/148‑1 (MQ=255)                                                                                  cTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTg                                                                     >  6:169055/1‑148 (MQ=255)                                                                                       aCAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGCCAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACaa                                                               <  1:315308/149‑1 (MQ=255)                                                                                       aCAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACaa                                                               <  1:204820/149‑1 (MQ=255)                                                                                         aaaGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATc                                                             <  1:118318/149‑1 (MQ=255)                                                                                           aGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATccc                                                           >  8:49680/1‑149 (MQ=255)                                                                                            ggCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATcccc                                                          >  2:370203/1‑149 (MQ=255)                                                                                               cGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCccgc                                                       >  1:9444/1‑149 (MQ=255)                                                                                                    cGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTg                                                  <  3:403702/149‑1 (MQ=255)                                                                                                      tgtTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTc                                                <  2:346933/149‑1 (MQ=255)                                                                                                       gtTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTcc                                               >  5:75938/1‑149 (MQ=255)                                                                                                           ccTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGCCCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                           >  4:408926/1‑149 (MQ=255)                                                                                                            cTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                           >  8:51779/1‑148 (MQ=255)                                                                                                             tGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                           >  1:351824/1‑147 (MQ=255)                                                                                                               gCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGgcag                                        <  5:436523/148‑1 (MQ=255)                                                                                                               gCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGgca                                         <  6:203107/147‑1 (MQ=255)                                                                                                                     ccccGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGAcc                                 >  6:386799/1‑149 (MQ=255)                                                                                                                       ccGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGt                               <  7:51779/149‑1 (MQ=255)                                                                                                                         gTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGCCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTAc                             <  7:319260/149‑1 (MQ=255)                                                                                                                          tACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACt                            <  2:9444/149‑1 (MQ=255)                                                                                                                           aCCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTc                           >  6:65547/1‑149 (MQ=255)                                                                                                                              cgcgCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGa                         >  6:409390/1‑148 (MQ=255)                                                                                                                               gcgcCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACa                       <  1:370203/149‑1 (MQ=255)                                                                                                                                  ccTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGc                     >  4:169903/1‑148 (MQ=255)                                                                                                                                   ctgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCg                    >  7:247494/1‑148 (MQ=255)                                                                                                                                   ctgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCg                    >  8:351107/1‑148 (MQ=255)                                                                                                                                    tgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGaa                  >  4:79714/1‑149 (MQ=255)                                                                                                                                      cacggcctGACCGCCGAGCAGGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACt                >  4:217317/8‑149 (MQ=255)                                                                                                                                        gTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGa              >  8:406577/1‑149 (MQ=255)                                                                                                                                         ttATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTg               <  3:214831/147‑1 (MQ=255)                                                                                                                                           aTTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGAtt            <  3:369245/148‑1 (MQ=255)                                                                                                                                            ttGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACACGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTc           <  8:247494/148‑1 (MQ=255)                                                                                                                                            ttGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACAGACCTCCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCTCTGTCCAGCGCAGCATGACCGTACTCGACAGCGAACTGATTcg          >  2:54720/1‑149 (MQ=255)                                                                                                                                              gACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGCCCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGACCTGATTcgcg        >  7:167554/1‑149 (MQ=255)                                                                                                                                              gACCGCCCTGCTCGGAGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTcgcg        >  4:337984/1‑149 (MQ=255)                                                                                                                                               aCCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCTGCATGACCGTACTCGACAGCGAACTGATTCgcgc       <  7:406577/149‑1 (MQ=255)                                                                                                                                               aCCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCgcgc       >  7:158164/1‑149 (MQ=255)                                                                                                                                                    ccTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCg  <  1:254599/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CGCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCG  >  NC_002947/4711669‑4711964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑