Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,711,818	T→A	6.3%	L17Q (CTG→CAG) ‡	PP_4171 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,711,818	0	T	A	6.3%	239.5 / 5.0	79	L17Q (CTG→CAG) ‡	PP_4171	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/32);  new base A (3/2);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCG  >  NC_002947/4711670‑4711964                                                                                                                                                     |                                                                                                                                                    gCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCt                                                                                                                                                     <  3:347427/149‑1 (MQ=255)          tACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggc                                                                                                                                              >  6:202520/1‑147 (MQ=255)          tACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggc                                                                                                                                              <  5:202520/147‑1 (MQ=255)             ttttCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCa                                                                                                                                         <  8:22998/149‑1 (MQ=255)                   aGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTc                                                                                                                                          >  6:292882/1‑142 (MQ=255)                   aGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTc                                                                                                                                          <  5:292882/142‑1 (MQ=255)                    gACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTg                                                                                                                                  >  1:346933/1‑149 (MQ=255)                             ccGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCgacg‑ac                                                                                                                        >  4:196792/1‑149 (MQ=255)                                acacCCACTAGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTg                                                                                                                     >  6:298386/1‑149 (MQ=255)                                  acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGTCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggcgg                                                                                                                                            >  6:139708/1‑125 (MQ=255)                                  acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGTCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTcggcgg                                                                                                                                            <  5:139708/125‑1 (MQ=255)                                  acCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGt                                                                                                                    >  4:369245/1‑148 (MQ=255)                                    ccACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCa                                                                                                                 <  6:59192/149‑1 (MQ=255)                                     cACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAg                                                                                                                                        >  2:298371/1‑126 (MQ=255)                                     cACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCAt                                                                                                                <  2:207954/149‑1 (MQ=255)                                        tGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTCa                                                                                                                                         >  4:247331/1‑122 (MQ=255)                                        tGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCGAGCAGGGCGGTCa                                                                                                                                         <  3:247331/122‑1 (MQ=255)                                            aaaaCCCCCACGGTGAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaac                                                                                                         <  2:7969/149‑1 (MQ=255)                                            aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                          >  5:203107/1‑148 (MQ=255)                                            aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                          >  4:58028/1‑148 (MQ=255)                                            aaaaCCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGacaa                                                                                                          >  8:99689/1‑148 (MQ=255)                                               acacccACGGTCATTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCTGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACAcc                                                                                                      <  6:241190/146‑1 (MQ=255)                                                cccccACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACAcc                                                                                                      >  1:123133/1‑148 (MQ=255)                                                  cccACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGAc                                                                                                   <  5:196908/149‑1 (MQ=255)                                                        gTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCca                                                                                             <  3:195609/149‑1 (MQ=255)                                                             ttACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGt                                                                                        >  5:166227/1‑149 (MQ=255)                                                               aCCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGtt                                                                                       <  3:196792/148‑1 (MQ=255)                                                                ccATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTc                                                                                      <  2:289500/148‑1 (MQ=255)                                                                  aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                   <  6:393854/149‑1 (MQ=255)                                                                  aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                   >  8:69802/1‑149 (MQ=255)                                                                  aTGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCGGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGa                                                                                   >  2:254599/1‑149 (MQ=255)                                                                     ccTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCg                                                                                <  5:298386/149‑1 (MQ=255)                                                                       tGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                               >  6:436523/1‑148 (MQ=255)                                                                          ttCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑CCCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCcaca                                                                           >  2:278563/1‑149 (MQ=255)                                                                          ttCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCcaca                                                                           >  1:427570/1‑149 (MQ=255)                                                                            cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                               >  7:361292/1‑143 (MQ=255)                                                                            cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGc                                                                               <  8:361292/143‑1 (MQ=255)                                                                            cAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCAACGACAACACCGACCTGCCACAGGTTCTGACCGCCACACg                                                                         >  1:200013/1‑149 (MQ=255)                                                                             aaTCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCacaccc                                                                        <  7:99689/149‑3 (MQ=255)                                                                               tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGGGGTCAGGCCGTGGCCGACGAAGAAGTTAATCCACAACACCGACAGGCCACAGGTTATGCCCCCCCCACGcc                                                                       >  1:142466/1‑149 (MQ=255)                                                                               tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCt                                                                      >  4:214831/1‑149 (MQ=255)                                                                               tCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCt                                                                      >  8:75864/1‑149 (MQ=255)                                                                                 cTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTg                                                                     >  6:169055/1‑148 (MQ=255)                                                                                 cTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTg                                                                     <  5:169055/148‑1 (MQ=255)                                                                                      aCAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGCCAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACaa                                                               <  1:315308/149‑1 (MQ=255)                                                                                      aCAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACaa                                                               <  1:204820/149‑1 (MQ=255)                                                                                        aaaGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATc                                                             <  1:118318/149‑1 (MQ=255)                                                                                          aGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATccc                                                           >  8:49680/1‑149 (MQ=255)                                                                                           ggCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATcccc                                                          >  2:370203/1‑149 (MQ=255)                                                                                              cGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCccgc                                                       >  1:9444/1‑149 (MQ=255)                                                                                                   cGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTg                                                  <  3:403702/149‑1 (MQ=255)                                                                                                     tgtTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTc                                                <  2:346933/149‑1 (MQ=255)                                                                                                      gtTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTcc                                               >  5:75938/1‑149 (MQ=255)                                                                                                          ccTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGCCCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                           >  4:408926/1‑149 (MQ=255)                                                                                                           cTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                           >  8:51779/1‑148 (MQ=255)                                                                                                            tGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCggg                                           >  1:351824/1‑147 (MQ=255)                                                                                                              gCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGgcag                                        <  5:436523/148‑1 (MQ=255)                                                                                                              gCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGgca                                         <  6:203107/147‑1 (MQ=255)                                                                                                                    ccccGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGAcc                                 >  6:386799/1‑149 (MQ=255)                                                                                                                      ccGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGt                               <  7:51779/149‑1 (MQ=255)                                                                                                                        gTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGCCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTAc                             <  7:319260/149‑1 (MQ=255)                                                                                                                         tACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACt                            <  2:9444/149‑1 (MQ=255)                                                                                                                          aCCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTc                           >  6:65547/1‑149 (MQ=255)                                                                                                                             cgcgCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGa                         >  6:409390/1‑148 (MQ=255)                                                                                                                              gcgcCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACa                       <  1:370203/149‑1 (MQ=255)                                                                                                                                 ccTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGc                     >  4:169903/1‑148 (MQ=255)                                                                                                                                  ctgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCg                    >  7:247494/1‑148 (MQ=255)                                                                                                                                  ctgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCg                    >  8:351107/1‑148 (MQ=255)                                                                                                                                   tgctgTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGaa                  >  4:79714/1‑149 (MQ=255)                                                                                                                                     cacggcctGACCGCCGAGCAGGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACt                >  4:217317/8‑149 (MQ=255)                                                                                                                                       gTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGa              >  8:406577/1‑149 (MQ=255)                                                                                                                                        ttATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTg               <  3:214831/147‑1 (MQ=255)                                                                                                                                          aTTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGAtt            <  3:369245/148‑1 (MQ=255)                                                                                                                                           ttGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACACGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTc           <  8:247494/148‑1 (MQ=255)                                                                                                                                           ttGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACAGACCTCCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCTCTGTCCAGCGCAGCATGACCGTACTCGACAGCGAACTGATTcg          >  2:54720/1‑149 (MQ=255)                                                                                                                                             gACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGCCCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGACCTGATTcgcg        >  7:167554/1‑149 (MQ=255)                                                                                                                                             gACCGCCCTGCTCGGAGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTcgcg        >  4:337984/1‑149 (MQ=255)                                                                                                                                              aCCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCTGCATGACCGTACTCGACAGCGAACTGATTCgcgc       <  7:406577/149‑1 (MQ=255)                                                                                                                                              aCCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCgcgc       >  7:158164/1‑149 (MQ=255)                                                                                                                                                   ccTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCg  <  1:254599/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GCCACGCGGTACTTTTCAAGACGTACGCCCGACACCCACTGGCAAAACCCCCACGGTCAGTTACCATGCCTGTTTCAATCCTGAAACAAAGGCCGCTCCGTGTTCCCTGGCACGCCCCCGTACCCGCGCCTGCTGTTATTGACCGCCCTGCTCGGCGGTCAGGCCGTGGCCGACG‑ACCTGTTCATCGACAACACCGACCTGCCACAGGTTCTGACCGCCACACGCCTGAAACAATCCCCCGCCGCTGTCCCGGGCAGCATGACCGTACTCGACAGCGAACTGATTCGCGCCAGCG  >  NC_002947/4711670‑4711964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑