| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | 100% | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 100.0% | 67.7 / NA | 22 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (18/4); total (18/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 66.7 / NA | 22 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (18/4); total (18/4) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG > NC_002947/1499443‑1499646 || cGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt > 2:279639/1‑148 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGa > 8:69406/1‑148 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGAc > 1:291652/1‑149 (MQ=25) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTcc > 4:326100/1‑149 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACGCCCt > 8:214760/1‑149 (MQ=37) gcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTTCCTCGCTAACGAATCATGACTCCCTTAg > 8:77899/1‑148 (MQ=37) gAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc > 6:418441/1‑149 (MQ=37) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 2:336296/1‑149 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 2:190340/1‑148 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 2:291652/149‑1 (MQ=37) cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa < 1:190340/149‑1 (MQ=37) tGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt < 7:77899/149‑1 (MQ=38) ggcaccGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc > 7:184138/6‑149 (MQ=38) gcaccGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACg > 6:89386/5‑149 (MQ=38) gcaccGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACg > 2:193153/5‑149 (MQ=38) caccGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGCTCTGAAACGt > 5:105679/4‑149 (MQ=38) accGGCTTTGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAGCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTc > 7:84495/3‑149 (MQ=38) cTTTGCCGGTGTTCGCGGGCACG‑CACGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTGTTTTGTTGCATCGCTATCGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAAGTCCCGAc > 2:300460/1‑149 (MQ=255) tttGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 3:377106/1‑149 (MQ=39) tttGCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 1:2793/1‑149 (MQ=39) gCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCg < 3:174828/143‑1 (MQ=255) gCCGGTGTTCGCGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCg > 4:174828/1‑143 (MQ=255) tttcgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg < 2:8208/144‑1 (MQ=255) cgcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg > 1:134375/3‑149 (MQ=255) gcGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgc > 7:68337/2‑149 (MQ=255) cGGGCACGCC‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg > 2:394045/1‑149 (MQ=255) || CGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑C‑CGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCG > NC_002947/1499443‑1499646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |