| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 92.7% | 133.5 / 4.3 | 41 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (18/20); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932502‑1932780 | tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTATGCGGCCCTTCGCGGGCAA‑GCCCGCACCTACAAAGGAAATGCGAACCCTGTACGAGCGGGC‑TT‑G‑CCACCGAAggg > 3:259310/1‑148 (MQ=38) aaGAGGATCTTGACATTCGACTGCGGCATTGCAGTTCTCACGAAAATCGAAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAACGGATATGCGGCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCt < 6:56754/147‑1 (MQ=37) aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAgg > 3:45658/1‑147 (MQ=255) aCATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGccc > 1:87072/1‑147 (MQ=255) cAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 5:322340/1‑147 (MQ=255) ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCAACAAAGGATATGCGTCCCCTGTTGGACCGGGCTTT‑C‑CC‑GCGAAGGGCAGCAAGGCACCCCCAAAATTTCGa > 5:175241/1‑146 (MQ=37) cATTGCAGGTCTAAGTAAAATAGCAGTAAACGGTGGGGCGGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGGGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAc < 2:80358/145‑1 (MQ=37) aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACt > 1:80358/1‑145 (MQ=255) ttGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcgc > 3:276367/1‑148 (MQ=255) ttGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcg < 5:125634/147‑1 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGTATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCa < 4:106136/148‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGc < 2:87072/145‑1 (MQ=255) gCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATTTGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAggg > 7:304054/1‑148 (MQ=255) ctgTAAACGCGGGGGCCGCTTTCGGGCCCTTCGCGGGCAC‑GCCCGCTCCTACAAAGGAAATGCGCCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt < 7:18942/146‑1 (MQ=37) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt > 3:266165/1‑148 (MQ=255) aaCGGTGGGGCCTCTTTGCGGCCCTGGGGGGTCAT‑GCCCTCTCGTACAATGTATATTCGTCCCCTTTTGGTGCTGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt < 4:259310/148‑1 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt < 5:246614/147‑1 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt < 6:246111/147‑1 (MQ=255) cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAg > 2:194668/1‑148 (MQ=255) ggCCGTTTTGCGGCCCTTCGGGGGCAC‑GCCCGCTCCTACAAAGGATATGCGGCCCCTGTAGGAGCGGGT‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTcc < 4:140237/148‑1 (MQ=39) gCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc > 8:247134/1‑146 (MQ=255) ccGCTTTGCGGCCGGTCG‑GGGCAACGCCCGCTCCAAAAAAGGATATGCGCCCCCTGTAGGAGGGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAg < 1:304325/148‑1 (MQ=37) ccGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCt > 6:132895/1‑144 (MQ=255) tggggCCCTCCGGGGCCAA‑CCCCGCTCCTAAAAAGTTTATGCGGCCCCTTTCGGAGGGGGG‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCg < 1:175327/144‑1 (MQ=255) gCGGCCCTTCGGGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa < 4:45658/147‑1 (MQ=255) cTTCGCGGGCAA‑GCCCGCTCCTAAAAAGGATTTGGGTCCCCTGTTGGAGCGGGC‑TTGG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTGGGCTACGCGGATCAGCGATCCGGCAGGGTGATGTTAAGCTACAGAATAGCGCAGCt > 6:102092/1‑147 (MQ=37) aaa‑GCCCGCTCCTAAAAAGTATTTGCGTCCCCTGTTGGTGCGGGT‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtt < 6:175241/146‑1 (MQ=255) a‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGt > 6:215405/1‑79 (MQ=255) a‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGt < 5:215405/79‑1 (MQ=255) tCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGAGATCCGGCAGGGTGATGGTCCGCTCCAGAATCGCGCCGCTGCCCTGGTTTTCCAGCTc > 3:267354/1‑147 (MQ=38) aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt > 1:292100/1‑127 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCt < 2:292100/127‑1 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 7:174491/1‑148 (MQ=255) cggATATCCGGCCCCTGTAGTCGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac < 7:107361/147‑1 (MQ=255) ttGCGCCCCCTGTCGGTGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat < 3:122922/147‑1 (MQ=39) gCGTCCCCTTTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcaa < 5:132895/146‑2 (MQ=255) cGTCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 3:318275/1‑148 (MQ=255) tCCCCTGTAGGAGCGGGC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 4:27873/1‑148 (MQ=255) ccccTGTAGGAGCGGGC‑TT‑GCCC‑CCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 5:320104/1‑147 (MQ=255) ggC‑TT‑GCCC‑GCGAGGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACatat > 7:275846/1‑148 (MQ=255) gC‑TT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAtt < 4:318275/148‑1 (MQ=255) | TCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAA‑GCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGC‑TT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATT > NC_002947/1932502‑1932780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |