| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 3,845,705 | +G | intergenic (‑72/+26) | PP_3394 ← / ← PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,845,703 | 1 | . | G | 95.3% | 142.5 / 0.7 | 43 | intergenic (‑70/+28) | PP_3394/PP_3395 | 3‑hydroxy‑3‑methylglutaryl‑CoA lyase/LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (25/16); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCA > NC_002947/3845557‑3845839 | gctgctGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGATAAGACGTGTTT‑c‑gc‑g > 1:141997/1‑148 (MQ=255) tgctgTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gg > 1:283468/1‑147 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGAt > 2:211837/1‑148 (MQ=255) tCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑gggctg < 8:264388/146‑5 (MQ=255) gCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATcc > 5:144435/1‑147 (MQ=255) tGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCg > 1:90880/1‑148 (MQ=255) aTCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGt > 1:24408/1‑147 (MQ=255) tCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGt > 1:43033/1‑146 (MQ=255) cACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAg > 2:215759/1‑148 (MQ=255) cAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGa < 8:175290/148‑1 (MQ=255) cAGGCTATTCAAGCCATCGCGCAGGCAGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACg < 7:30416/147‑1 (MQ=255) ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATg < 2:310925/148‑1 (MQ=255) ggCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATg > 4:111799/1‑148 (MQ=255) gCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTGGCTGACGATGGATCAGCCcg < 1:127252/148‑1 (MQ=38) gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTg < 5:79366/147‑1 (MQ=255) gcAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATACATAGTCAGCCACGATGGATCAGCCCGCGCCCGGc > 2:166752/1‑148 (MQ=255) cGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGc > 6:270472/1‑147 (MQ=255) ttgattgTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACg > 8:162516/1‑148 (MQ=255) ttttttattgttGTCATCGCGGTCTCTCTGTCGCAAGCGTGGCGCCAGACTAAGCGCTGGATGAGGGGCACAAAACCGGTGTTT‑C‑GCGGGCTGACCCATCGTCCGCCACGTTGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACgca < 2:90880/143‑1 (MQ=255) ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa > 3:197802/1‑144 (MQ=255) ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCaa < 4:197802/144‑1 (MQ=255) ttgttgTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAg < 7:162516/147‑1 (MQ=255) tgttgtCATGGGGGTCTCTGTGTCTGAGGCTTGGCGCCAGACTAAGTGGTGTTTGAGGGGCATAAAAGACGTGTTTGG‑GG‑GGCTGATCCATCGTCCGCCACGATGGATCTGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggt < 8:63324/148‑1 (MQ=255) tgtCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAggtg > 7:63324/1‑146 (MQ=255) aTCGCGGTCTCTCTGTCTTAAGCGTTGCGCCTGACTAAGAGCTGGTTGACGGGCTGAAACGCGGTGTTT‑G‑GCGGGCTTATCCTTCGTCAGCCAGGAGGCATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCaa < 1:14683/147‑1 (MQ=255) tCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGc < 2:43033/148‑1 (MQ=255) cgcgGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATACCTCGTGAGCCCCGATGGATCACCCCCCGCCCGGCCAGGCCGGGGCACCGAGCAACAGGTGGTGaa > 5:224013/1‑145 (MQ=255) ctgtctgAAGCGTTGCGCCTTACTAATAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGTTGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCgcc < 6:144435/144‑1 (MQ=255) ctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTTAAACATAGTAAGCCACGATGGATAAGCCCCCCCCCTGCCAGGCCGGTGGACGCAGCAACAGGTGGCCCAGCCGGCgccgagc > 2:126504/1‑148 (MQ=255) ctgtctgAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGCCAAGCAGGCgccgagc > 8:165643/1‑148 (MQ=255) gAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCATGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACa > 5:176766/1‑147 (MQ=255) aGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGc > 8:186328/1‑147 (MQ=255) ccAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCgtgt > 3:86260/1‑148 (MQ=255) cAGACTAAGAGCTGGATGACGGGCAGAAAAGCCGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGtgtg < 1:211837/148‑1 (MQ=255) tAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATACATCGTCAGCCACGATGGATCAGCCCTAGCCATGCCAGGCCGGTGCAAGCAGCAACAGGTGGTAAAGCAGTCGCCGAGCCGACAGCGAGAGCGTGTGCCgg > 3:300794/1‑147 (MQ=255) gCTGTATGACGGGCATAAAATATGTGTTT‑CGGG‑GGCTGATCCTTCGTCAGCCACTTTGTATCTGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTcgcg < 4:86260/147‑1 (MQ=255) tGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCa > 5:331326/1‑147 (MQ=255) tGGATGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCAt > 2:205693/1‑148 (MQ=255) ttGACGGGCATAAAAGACGTGTTT‑C‑GCGGGCTTATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg < 6:99148/145‑1 (MQ=255) aTGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTTAAACATCCTGAGCCACCATGGAAAACACCCCGCCCAACCAGGCCCGTGCACGCAGCAACAAGTGGGCAAGCACGCCCCGAGCCGACACCGAGAGCGGGTTCCCGTCGAGCATGc > 4:179041/1‑147 (MQ=255) aTGACGGGCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATg > 5:99148/1‑146 (MQ=255) gCAGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGAc > 8:16756/1‑147 (MQ=255) aGAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGTATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGa < 5:270472/147‑1 (MQ=255) gAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGa < 6:313126/143‑1 (MQ=255) gAAAAGACGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGa > 5:313126/1‑143 (MQ=255) cGTGTTT‑C‑GCGGGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCa < 7:186328/147‑1 (MQ=255) | GCTGCTGTCGGCATGATCACTTGCAGGCTCTGCAAGCCATCGCGCAGGCCGACTTCATTGATTGTTATTGTTGTCATCGCGGTCTCTCTGTCTGAAGCGTTGCGCCAGACTAAGAGCTGGATGACGGGCAGAAAAGACGTGTTT‑C‑GC‑GGCTGATCCATCGTCAGCCACGATGGATCAGCCCGCGCCCTGCCAGGCCGGTGCACGCAGCAACAGGTGGTCAAGCAGGCGCCGAGCCGACAGCGAGAGCGTGTGCCGGTCGCGCATGCAGATGACGAATTCACCA > NC_002947/3845557‑3845839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |