| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 307,866 | +C | intergenic (+881/‑988) | hslO → / → PP_0254 | molecular chaperone HslO/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 307,865 | 1 | . | C | 98.0% | 170.6 / ‑4.7 | 50 | intergenic (+880/‑989) | hslO/PP_0254 | molecular chaperone HslO/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (24/25); minor base A (1/0); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
CCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG > NC_002947/307721‑308004 | ccATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAG‑ccg < 4:126288/148‑3 (MQ=255) tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAG‑ccgtta > 4:143840/1‑143 (MQ=255) tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAG‑ccgtt > 6:304827/1‑143 (MQ=255) ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATc < 1:23960/148‑1 (MQ=255) gTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCg > 8:80120/1‑148 (MQ=255) tGCCGAACTTCGTGCTGCCGGGTGCCGATGTTCTGGCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACATGATCgg < 8:131674/148‑1 (MQ=255) cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGTGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGt > 1:135166/1‑147 (MQ=255) cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGt < 4:163368/147‑1 (MQ=255) aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTg > 5:70976/1‑147 (MQ=255) aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTg < 2:295823/147‑1 (MQ=255) aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTgacg < 7:80120/148‑1 (MQ=255) aCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTgacga > 3:281089/1‑148 (MQ=255) gctgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCAc > 4:249955/1‑148 (MQ=255) ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGtt < 7:55420/148‑1 (MQ=255) ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGtt < 3:19161/148‑1 (MQ=255) ggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGATGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTgg > 2:298981/1‑148 (MQ=255) tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCg > 5:114746/1‑147 (MQ=255) aCTGCCTATGCCCTGCGCTGCCAACCTCTGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCg < 6:170570/146‑1 (MQ=255) gCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTc > 1:209090/1‑148 (MQ=255) ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCt > 8:212504/1‑148 (MQ=255) tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa < 8:29561/148‑1 (MQ=255) cTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGCTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACAt > 6:116061/1‑147 (MQ=255) cgcgGCCAACATCGGCGAAGAAGGTGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGa < 2:135166/147‑1 (MQ=255) aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt < 5:121547/125‑1 (MQ=255) aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGt > 6:121547/1‑125 (MQ=255) gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCa < 3:143840/148‑1 (MQ=255) gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGACGTTACCTGATCGGTGACGACGAGCACGCTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGcc > 7:290787/1‑147 (MQ=255) aagaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTCACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCACCATGGAAGGCGGCTGCTACGCCag > 8:229446/1‑147 (MQ=255) agaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg > 8:254006/1‑148 (MQ=255) gaagGCGATGTGAGCTTGTTCTTCGTCCTGTCCGGCACTGTCAAGACTACCCTTTCGGCTTACG‑AAAGCCGTTACCTGATCGGTGACGAGGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg < 6:149893/147‑1 (MQ=255) agGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGc < 7:212504/148‑1 (MQ=255) gCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCATTCGGCTCACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGCCTGCTACGCCAAGTGCa > 5:246568/1‑147 (MQ=255) aTGTGTCCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa < 4:281577/148‑1 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGTCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa < 2:62835/148‑1 (MQ=255) aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGa < 2:209090/148‑1 (MQ=255) ttGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACTTGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc > 1:247094/1‑148 (MQ=255) ttGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATc > 6:23897/1‑66 (MQ=255) ttGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc > 7:59762/1‑147 (MQ=255) ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag > 1:227394/1‑147 (MQ=255) tcGGCCTGTCAGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa > 4:52114/1‑148 (MQ=255) ggCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa < 6:114746/146‑1 (MQ=255) cTGTCCGGCACTGGCAAGACTAACCTTTCGGATGACG‑AAAGCCGTTACATGATCGGAGACGACGAGCACGGATGGGGCGAAGGCATTCTCAACAACATTGAAGGCTGCTGCTAAGCCAAATGCATCGACCTGAACGAGAAGAACGAg > 6:292007/1‑147 (MQ=255) gTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag > 7:151349/1‑139 (MQ=255) gTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag < 8:151349/139‑1 (MQ=255) tCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGcc < 4:281089/146‑1 (MQ=255) gCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCAt > 3:280771/1‑148 (MQ=255) gCAAGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTgg < 3:72308/146‑1 (MQ=255) aGACTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGc > 1:39087/1‑148 (MQ=255) cTACCCTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt < 2:270793/148‑1 (MQ=255) cccTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCa < 1:298981/147‑1 (MQ=255) cTTTCGGCTGACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGc < 4:280771/148‑1 (MQ=255) gggtGACG‑ATAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGgc < 3:276119/145‑1 (MQ=255) gACG‑AAAGCCGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAg > 8:293761/1‑148 (MQ=255) | CCATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACG‑AAAG‑CGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAG > NC_002947/307721‑308004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |