Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,283	+T	intergenic (+520/+233)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,283	1	.	T	100.0%	95.4 / NA	29	intergenic (+520/+233)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (14/15);  total (14/15)

GTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTG  >  NC_002947/4741137‑4741404                                                                                                                                                     |                                                                                                                           gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                                                            <  3:146502/148‑2 (MQ=255)     tAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgcag                                                                                                                        >  7:125997/1‑143 (MQ=255)            cagcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctc                                                                                                                  >  3:218558/1‑145 (MQ=255)                           cggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgcggc                                                                                                 >  2:289073/1‑148 (MQ=255)                                   ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGa                                                                                          >  5:325546/1‑147 (MQ=255)                                       ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACg                                                                                     <  7:175817/148‑1 (MQ=255)                                           ggCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTca                                                                                 >  7:223028/1‑148 (MQ=255)                                            gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTcac                                                                                <  1:83158/148‑1 (MQ=255)                                               tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                                             <  4:218558/148‑1 (MQ=255)                                                   gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACcgcc                                                                          >  5:223998/1‑146 (MQ=255)                                                     aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                       <  4:160248/148‑1 (MQ=255)                                                         ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                                   <  1:36282/148‑1 (MQ=255)                                                          gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                  >  1:49490/1‑148 (MQ=255)                                                           tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTTCGACTTCCATTCGTGAATCAAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TTGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                                 <  3:200424/148‑1 (MQ=255)                                                           tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                     >  3:10943/1‑144 (MQ=255)                                                           tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                     <  4:10943/144‑1 (MQ=255)                                                             taGGTCCAGCGCTGCTCACCTTCGTCCTTGAGCAGCTTCTGCGACATGCAA‑TCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACTCCGCGATAGGCCt                                                                  <  8:253866/145‑1 (MQ=255)                                                              aGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcct                                                                                                                   >  3:201997/1‑95 (MQ=255)                                                              aGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcct                                                                                                                   <  4:201997/96‑2 (MQ=255)                                                                gTCCATCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                     <  1:63111/140‑1 (MQ=255)                                                                 tCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                    >  2:63111/1‑139 (MQ=255)                                                                    aGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGAGTTGC‑ATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGa                                                        <  8:125997/148‑1 (MQ=255)                                                                          ggTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc                                                  >  1:225365/1‑148 (MQ=255)                                                                            tCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                >  4:272927/1‑148 (MQ=255)                                                                            tCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                <  8:223028/148‑1 (MQ=255)                                                                            tCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                >  1:154504/1‑148 (MQ=255)                                                                                                        gacttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc                        <  6:325546/138‑1 (MQ=255)                                                                                                         acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc                    >  2:194158/7‑148 (MQ=255)                                                                                                                          caggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACt    <  1:289073/140‑1 (MQ=255)                                                                                                                           aggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTg  <  1:194158/142‑1 (MQ=255)                                                                                                                            ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTg  >  6:306960/6‑147 (MQ=255)                                                                                                                                                     |                                                                                                                           GTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTG  >  NC_002947/4741137‑4741404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑