Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,751	+G	intergenic (+113/+27)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,750	1	.	G	100.0%	163.3 / NA	46	intergenic (+112/+28)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (22/24);  total (22/24)

GTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674604‑5674890                                                                                                                                                    |                                                                                                                                               gTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑g                                                                                                                                               >  1:266215/1‑148 (MQ=255)  tGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑gg                                                                                                                                              <  3:6745/148‑2 (MQ=255)    aGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCACAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggc                                                                                                                                             <  1:63264/147‑3 (MQ=255)                 gctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGtt‑tt                                                                                                                                 >  4:86737/1‑146 (MQ=255)                      cccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTgg                                                                                                                          <  7:115434/148‑1 (MQ=255)                        cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCGCCGTT‑TTCAGCTg                                                                                                                           >  5:47575/1‑145 (MQ=37)                          cAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGa                                                                                                                      <  2:175145/148‑1 (MQ=255)                                cTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTAc                                                                                                                >  4:980/1‑148 (MQ=255)                                   aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACtt                                                                                                              <  4:297357/147‑1 (MQ=255)                                    tAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGACTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTAGCAGAAATTACTTc                                                                                                             >  5:187051/1‑147 (MQ=37)                                    tAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGGGTCGTGATAGTGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGAAGAAATTACTTCt                                                                                                            >  3:272861/1‑148 (MQ=37)                                        aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTTATCTCTACAGGAGCGGCCTTGTGTCTTGATAGGGCCGCAAAGCGGCCCCCTCGGTTCTTCAGCTGGCAGAAATTACTTCTGCt                                                                                                         <  1:183002/148‑1 (MQ=37)                                        aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCt                                                                                                         <  6:206342/147‑1 (MQ=255)                                              gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACAcc                                                                                                  >  2:205531/1‑148 (MQ=255)                                                gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACGTCTGCTTCACACCtt                                                                                                >  1:65401/1‑148 (MQ=255)                                                          tCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGa                                                                                       >  4:94744/1‑147 (MQ=255)                                                                   tATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TAtccaggtc                                                                            >  7:223696/1‑148 (MQ=255)                                                                    aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCt                                                                                                            >  6:319018/1‑116 (MQ=255)                                                                    aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCt                                                                                                            <  5:319018/116‑1 (MQ=255)                                                                     tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCAAACATTCGACACTGA‑TAtccaggtc                                                                            >  8:112576/1‑146 (MQ=38)                                                                          acacAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTc                                                                     <  8:245345/148‑1 (MQ=255)                                                                               atgcagCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGa                                                                <  3:5649/143‑1 (MQ=255)                                                                               aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCg                                                                 >  2:161402/1‑147 (MQ=255)                                                                                ggCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGaa                                                               <  7:270102/148‑1 (MQ=255)                                                                                  ccGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGt                                                             <  2:266215/148‑1 (MQ=255)                                                                                     cTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAg                                                           >  3:71387/1‑147 (MQ=255)                                                                                        cTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCa                                                         <  3:86737/146‑1 (MQ=255)                                                                                           cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCAccc                                                      <  2:114554/146‑1 (MQ=255)                                                                                           cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                     <  3:93197/147‑1 (MQ=255)                                                                                           cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                     <  3:980/147‑1 (MQ=255)                                                                                           cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACCTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                     <  2:65401/147‑1 (MQ=255)                                                                                                        gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        >  8:12793/1‑147 (MQ=255)                                                                                                              tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGa                                      <  3:94744/143‑1 (MQ=255)                                                                                                                    aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                           >  8:128457/1‑148 (MQ=255)                                                                                                                       ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCGCTCGGTT‑TTCAGCTGGCTTAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCGAGGGTCTGCGAAGTAGCACGCGGGCCTTTGATGCCGAAGTCgttctggttc                        <  4:272861/148‑1 (MQ=255)                                                                                                                       ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggtt                         <  6:47575/147‑1 (MQ=255)                                                                                                                       ggCCTTGGTTCGTGATAGGGCCTCAAAGCGGCCCCCTCTGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttc                        <  4:71387/148‑1 (MQ=255)                                                                                                                        gCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                       <  1:205531/148‑1 (MQ=255)                                                                                                                           ttGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCTGAAGTCGTTCAGGTTCAGCg                    >  7:1122/1‑148 (MQ=255)                                                                                                                                       aGGGCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCg         <  6:119262/147‑1 (MQ=255)                                                                                                                                          gCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  7:37051/1‑147 (MQ=255)                                                                                                                                          gCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa     <  7:248401/148‑1 (MQ=255)                                                                                                                                          gCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa     <  2:275070/148‑1 (MQ=255)                                                                                                                                          gCCGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCAAACCTTCGCCACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGtt       >  6:279565/1‑146 (MQ=255)                                                                                                                                           ccGCAAAGCGGCCCCCTCGGTT‑TTCAGCTAGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGa     <  6:187051/147‑1 (MQ=255)                                                                                                                                            cGCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg   >  2:176086/1‑148 (MQ=255)                                                                                                                                             gCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGAGTATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAg   >  1:98679/1‑148 (MQ=255)                                                                                                                                             gCAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  8:80514/1‑148 (MQ=255)                                                                                                                                              cAAAGCGGCCCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  4:138298/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                               GTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTT‑TTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGA‑TATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674604‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑