Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,126,647 +C intergenic (‑101/+48) tdcG‑II ← / ← gcvP‑I L‑serine dehydratase/glycine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,126,6451.C97.7% 151.2 / ‑3.5 44intergenic (‑99/+50)tdcG‑II/gcvP‑IL‑serine dehydratase/glycine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (18/25);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGT  >  NC_002947/1126502‑1126786
                                                                                                                                                  |                                                                                                                                             
ggggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAA‑c                                                                                                                                              <  7:117538/145‑1 (MQ=255)
 gggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCGCGAATGCGGAGGTATTGCCACCATCGCAATAG‑CGGGGTAAA‑c                                                                                                                                              >  8:117538/1‑145 (MQ=255)
                           gAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAg                                                                                                                  <  7:272368/147‑1 (MQ=255)
                            aaGACGCTCAGTGACATGTGCACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTAACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGcc                                                                                                                <  7:205130/148‑1 (MQ=255)
                                     aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGc                                                                                                         <  7:189903/146‑1 (MQ=255)
                                           aTGTGGACCTCCTGCCAGGTTGGTGGGTGGACATTGAGCCGTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATACCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg                                                                                                 <  4:101209/148‑1 (MQ=38)
                                            tgtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCCCCAATGCGGAGGTATAGGCACCATCGCATTAG‑C‑GGGTAAACCCGCTCCGTCACAGATCCCCCCCCCCAAGCCAGGTGGCGgggcgggc                                                                                                >  7:221030/1‑148 (MQ=255)
                                             gtgGACCTCCTGCTAGGTTGTTGGGTGTACATTGAGCCGTGTAGGCGGGGGTTTACCGGCGAATGCGGAGTTATAGCCACCATCGCATTCG‑G‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGc                                                                                                <  4:291315/147‑1 (MQ=38)
                                              ggtccctcctGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGTTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCt                                                                                               <  8:182292/143‑1 (MQ=255)
                                               ggACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt                                                                                             <  3:24004/148‑1 (MQ=255)
                                               gcaCCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAAGGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAt                                                                                             <  3:23696/146‑1 (MQ=255)
                                                          cAGGTGGTGGGGGGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCTAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTc                                                                                     <  6:276123/145‑1 (MQ=37)
                                                            ggTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTAACCCCGAATGCGGAGGTTTATGCCCCATAGCATTAGCC‑GGTTAAC‑CCGCTCCTACACAGAGCCCCCCCCCCAACCCACGTGGCGGGGAGGGCTATCACGCGTCCAGa                                                                                 >  7:95676/1‑147 (MQ=255)
                                                                 ttgGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGctc                                                                           <  7:150591/148‑1 (MQ=255)
                                                                           aTTGCGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATCGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACg                                                                 <  3:217554/148‑1 (MQ=255)
                                                                            ttGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACgg                                                                <  2:15367/148‑1 (MQ=255)
                                                                                gCCCTGTAGGCGCGGGTTTAACCCCGAATGCGGAGGTATAGCCCCCATCGCATTAG‑C‑GGGTAAACCCCCTCCAACAAAGAGCCCCCCACCCCACCCACGAGGCGGGGAGGGCAATCAGGCGTGCTGATATCGATCGATACACGGGCa                                                             >  6:145416/1‑147 (MQ=255)
                                                                                  ccTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGgc                                                          <  1:283548/148‑1 (MQ=255)
                                                                                        ggCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCacac                                                    <  3:22942/148‑1 (MQ=255)
                                                                                            cgGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAg                                                 >  6:312786/1‑147 (MQ=255)
                                                                                              ggTTTACCCGCGAATGCCGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGt                                               <  6:234126/147‑1 (MQ=255)
                                                                                               gTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTg                                             <  1:133343/148‑1 (MQ=255)
                                                                                                 ttACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTCATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTAGCg                                           >  2:258032/1‑148 (MQ=255)
                                                                                                    cccGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTAACAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTc                                        >  7:53622/1‑148 (MQ=255)
                                                                                                      cgcgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                      >  2:20582/1‑148 (MQ=255)
                                                                                                       gcgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                      <  5:145416/147‑1 (MQ=255)
                                                                                                        cgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgatcga                                                                   >  7:62629/1‑117 (MQ=255)
                                                                                                        cgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgatcga                                                                   <  8:62629/117‑1 (MQ=255)
                                                                                                        cgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc                                     >  2:58388/1‑147 (MQ=255)
                                                                                                        cgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc                                     >  2:166348/1‑147 (MQ=255)
                                                                                                        cgAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc                                      >  5:115458/1‑146 (MQ=255)
                                                                                                                        gCCACCATCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAcc                     >  6:298941/1‑147 (MQ=255)
                                                                                                                          caccaTCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg                  >  7:55644/1‑148 (MQ=255)
                                                                                                                          caccaTCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg                  >  3:37163/1‑148 (MQ=255)
                                                                                                                            ccaTCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc                    <  7:120686/144‑1 (MQ=255)
                                                                                                                            ccaTCGCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGAccc                    >  8:120686/1‑144 (MQ=255)
                                                                                                                              aTCGCATTCG‑G‑GGGTAAACCCGCTCCTACACAGAGCCCGCCAACCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGAGGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa              <  8:221030/148‑1 (MQ=255)
                                                                                                                                 gCATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAcc            <  4:37163/147‑1 (MQ=255)
                                                                                                                                  cATTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcgg          <  8:55644/148‑1 (MQ=255)
                                                                                                                                   aTTCG‑C‑GGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACCCCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc         >  1:119576/1‑148 (MQ=255)
                                                                                                                                       g‑c‑gGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGTGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGcc     <  8:53622/148‑1 (MQ=255)
                                                                                                                                         c‑gGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc      >  1:113554/1‑146 (MQ=255)
                                                                                                                                         c‑gGGTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGcc     <  5:251087/147‑1 (MQ=255)
                                                                                                                                           gggTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg   >  3:88341/1‑148 (MQ=255)
                                                                                                                                            ggTAAACCCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGt  <  8:95676/148‑1 (MQ=255)
                                                                                                                                            ggTAAACCCGCTCCTACACAGAGCCCCCCACCCAAGCCAGGTGGAGGGGAGGGCTATCAGGCGTCCAGATACCTCACGATCGACGGGCAGGCGCACACCACGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACAGGCGGCCAg   >  8:67724/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGTTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAA‑CCGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAGT  >  NC_002947/1126502‑1126786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑