Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	0 (0.000)	26 (0.470) +CGGG	23/274	1.5	100%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	0 (0.000)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

TCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACC  >  NC_002947/5681269‑5681561                                                                                                                                                   |                                                                                                                                                   tCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACcgc                                                                                                                                                    >  5:140152/1‑147 (MQ=255)  cACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCc                                                                                                                                                   <  8:11197‑M1/147‑2 (MQ=255)       tAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggct                                                                                                                                             <  7:298659‑M1/148‑8 (MQ=255)           aCATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCAAGAACCGCcggggcagctg                                                                                                                                         >  8:219432‑M1/1‑137 (MQ=255)               gCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgc                                                                                                                                      <  5:6165‑M1/147‑15 (MQ=255)                cAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTTTGTGGCGAAAGGGGCGCGAAGCGGCCCCAGGCGTTCATTGCCACTGCATGCACAGCcgggggagctgcgccg                                                                                                                                    >  8:276156‑M1/1‑132 (MQ=255)                  aaCACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCc                                                                                                                                                   >  3:141773‑M1/1‑130 (MQ=255)                  aaCACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCc                                                                                                                                                   <  4:141773‑M1/131‑2 (MQ=255)                    cacaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTTTGTAGCGAAAGGGGCGCGAAGCGGCCCCCGGCTTTCATTTACACTGCATGAACCGCcggggctgctgggccgccc                                                                                                                                 >  3:233194‑M1/1‑128 (MQ=255)                     acaGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagcccta                                                                                                                               <  3:145666‑M1/148‑22 (MQ=255)                       aGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctat                                                                                                                              <  5:72423‑M1/147‑23 (MQ=255)                         aCCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcg                                                                                                                            <  5:138191‑M1/147‑25 (MQ=255)                                 aGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacaca                                                                                                                     <  3:179130‑M1/146‑32 (MQ=255)                                                  gttgtGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgcttcgcagccctatagcgacacaaggccgctccccctccaga                                                                                                  >  7:90568‑M1/1‑98 (MQ=255)                                                        aTGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTGGCGCAAGGGGCGCGAAGCGGCCCCAGGCTTTAATTGCCACTGCATGAACCGCcggggcggctgggccgcccgataccgccacaaggccgcggccactccagaccac                                                                                              >  3:75119‑M1/1‑92 (MQ=255)                                                        aTGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatagcgacacaaggccgcgaccccaccagaccac                                                                                              >  6:310218‑M1/1‑92 (MQ=255)                                                                         cTTTGTGGGAGCGGCCCTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcaca                                                                            <  7:253012‑M1/147‑73 (MQ=255)                                                                             gtgGGAGCGGCCTTGTGTCGCTAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGACCCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcct                                                                        <  7:219432‑M1/147‑77 (MQ=255)                                                                                               cgcgAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaagggcgctcccacaccagaaccccaggccctgcgccgcacatcataaccaagttcaggttg                                                        >  1:235038‑M1/1‑53 (MQ=255)                                                                                               cgcgAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgccacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttg                                                        >  1:199394‑M1/1‑53 (MQ=255)                                                                                                          cgcgAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccaga                                                                                                  <  6:168815‑M1/92‑51 (MQ=255)                                                                                                             cacgcggCCCCAGGCTTTCATTCCCACTCCATGAACCGCcggggctgctgcgcagcccaatcgctacaaaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgca                                       <  7:276156‑M1/145‑110 (MQ=255)                                                                                                                                  tGCCACTGCATGAACCGCcggggctgctgcgccgccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcga                     >  6:113342‑M1/1‑18 (MQ=255)                                                                                                                                  tGCCACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacaccaggccgcgccccctccagaccaccaggccctgggcctcacatccgaaccaagggcaggttgcagaagcgcgtgacgcaccagccgttccaatgcgac                    >  8:197348‑M1/1‑18 (MQ=255)                                                                                                                                     cACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggt               <  4:278379‑M1/148‑134 (MQ=255)                                                                                                                                     cACTGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggt               >  3:278379‑M1/1‑15 (MQ=255)                                                                                                                                        tGCATGAACCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttcaaatgcgaccaggtcc             >  6:104889‑M1/1‑12 (MQ=255)                                                                                                                                           aTGAACCGCcggggctgctgcgcagccctatcgctacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccgg           <  6:140152‑M1/146‑138 (MQ=255)                                                                                                                                               aCCGCcggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttcaaatgcgaccaggtccggtacc       <  5:104889‑M1/146‑142 (MQ=255)                                                                                                                                                   ccggggctgctgcgcagccctatcgcgacacaaggccgctcccactccagaccaccaggccctgcgcctcacatcctaaccaaggtcaggttgcagatccgcgtcacgcaccagcctttccaatgcgaccaggtccggtacccgcgc  >  5:133494‑M1/1‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   TCACCGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCGCTGCTGCGCAGCCCTATCGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTCCGGTACCCGCGCCACC  >  NC_002947/5681269‑5681561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.