| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,988,905 | 0 | T | C | 28.0% | 70.3 / 33.3 | 50 | intergenic (+199/‑347) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base T (18/18); new base C (5/9); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGC > NC_002947/5988761‑5989047 | cgcgcgGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTgg < 3:67584/148‑1 (MQ=255) ggcgCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGa < 3:282196/148‑1 (MQ=255) cgcgGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaacaa < 1:22378/146‑1 (MQ=255) gggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaaccaacag > 4:171093/1‑143 (MQ=255) ggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTG‑CGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAgg < 7:214881/148‑1 (MQ=255) tAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAg > 8:57541/1‑146 (MQ=255) ccGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCAAGCGA‑‑‑‑GCCA‑CCTGGA‑‑GCACGCCTGGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCAAGAAACAAACGTAGGAgagcg > 1:71492/1‑144 (MQ=25) cGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGCGTGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCgg > 1:259294/1‑148 (MQ=255) cGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCggg > 5:11852/1‑147 (MQ=255) tCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGA‑‑‑‑CCGC‑CTTGGG‑‑GCATGTCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGACTGGTGGAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAc > 7:219508/1‑148 (MQ=11) cGTTTGTTTCGGGCCATTTATTCCTGTGGGACGCGCAAGCGATCCTACCCACCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAACGCGGT‑CGCGCGCTCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTa < 1:111269/148‑1 (MQ=255) tttgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcga < 7:269750‑M2/148‑8 (MQ=255) tgtttCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCGGCG‑‑GCAGGCCTCTATATCGCTTCTTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGTATTGGCCAGAAACAAACGTAGTAGCGGGTTTAcccgcga > 6:263051‑M2/1‑139 (MQ=255) tCCTGCGGGATTTGCGCGCGA‑‑‑‑CCGC‑CTTGGG‑‑GCATGTCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCt > 7:150316/1‑148 (MQ=14) gtgGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctc < 1:262938‑M2/147‑29 (MQ=255) gtgGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga < 1:50628‑M2/148‑31 (MQ=255) tgGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGACACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcga < 5:138533‑M2/147‑31 (MQ=255) gACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatc < 5:7107‑M2/146‑33 (MQ=255) cgcgA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGgcgc > 4:38987/1‑148 (MQ=255) cgcgA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTAGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGgcg > 6:325573/1‑147 (MQ=255) accgc‑cttggg‑‑GCATGTCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaa < 2:15927‑M2/143‑47 (MQ=255) ccc‑ccTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaa < 2:262938/148‑1 (MQ=255) ccc‑ccTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCaaaa < 2:50628/148‑1 (MQ=255) gc‑cttGGG‑‑GCATGTCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAAAGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggctctgaaaat < 6:264173‑M2/146‑50 (MQ=255) cTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTa > 7:207485/1‑148 (MQ=255) gtctcGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaaca < 8:233809‑M2/146‑63 (MQ=255) gATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTg > 3:113060/1‑148 (MQ=255) atatCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACACACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTCTCGCCAGGCACCt > 6:234807/1‑146 (MQ=255) cgcgTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGc > 3:285632/1‑148 (MQ=255) gcgTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCGGCAAGc > 2:296574/1‑147 (MQ=255) cgtcgtACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCa < 7:57541/148‑1 (MQ=255) acaaacaaGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaa > 8:219413‑M2/1‑68 (MQ=255) acaaacaaGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaa < 7:219413‑M2/137‑70 (MQ=255) caaacaaGGCGGT‑CGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccg < 8:171791‑M2/148‑82 (MQ=255) acaaGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAAtt > 2:329265/1‑148 (MQ=255) aGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAACCTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTcc > 7:240475/1‑148 (MQ=255) aGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTcc > 1:188661/1‑148 (MQ=255) gCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTg < 4:285632/148‑1 (MQ=255) cGGT‑CGCGCGCGCCTGGCACAGGAGGGATTAGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTg < 5:325573/147‑1 (MQ=255) gcgcgcgaCTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaatt > 7:233809‑M2/1‑53 (MQ=255) tGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgcc < 6:11852/147‑1 (MQ=255) gCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCgccgcc < 8:207485/148‑1 (MQ=255) gCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCgccgcc < 2:259294/148‑1 (MQ=255) aggaggGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctggatctcacaggcgctgaaaatcctccggcaaacccctccaaattccaccggcgtcgcctccaaattccaccgccgcccccgc < 2:308248‑M2/148‑111 (MQ=255) gaggGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATAACAGCGCCGCCATGAc > 6:170648/1‑147 (MQ=255) gaggGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctca < 3:242638‑M2/147‑112 (MQ=255) aggGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGAcgc > 4:91739/1‑148 (MQ=255) aggGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtccactcaaaattccaacgccgccccctca < 6:25842‑M2/146‑112 (MQ=255) aggGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgct > 6:80596‑M2/1‑35 (MQ=255) aggGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgct < 5:80596‑M2/62‑28 (MQ=255) | CGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGA‑‑‑‑GCCC‑CCTGGC‑‑GCACGCCTCGATATCGCGTCGTACAAACAAGGCGGT‑CGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGC > NC_002947/5988761‑5989047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |