Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,294	C→G	intergenic (+531/+222)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,294	0	C	G	100.0%	87.9 / NA	26	intergenic (+531/+222)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/14);  total (12/14)

CGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTG  >  NC_002947/4741163‑4741404                                                                                                                                      |                                                                                                                cggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAgcggc                                                                                                 >  2:289073/1‑148 (MQ=255)         ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGa                                                                                          >  5:325546/1‑147 (MQ=255)             ccATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACg                                                                                     <  7:175817/148‑1 (MQ=255)                 ggCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTca                                                                                 >  7:223028/1‑148 (MQ=255)                  gCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTcac                                                                                <  1:83158/148‑1 (MQ=255)                     tATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACAcc                                                                             <  4:218558/148‑1 (MQ=255)                         gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACcgcc                                                                          >  5:223998/1‑146 (MQ=255)                           aCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                                       <  4:160248/148‑1 (MQ=255)                               ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                                   <  1:36282/148‑1 (MQ=255)                                gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCt                                                                  >  1:49490/1‑148 (MQ=255)                                 tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTTCGACTTCCATTCGTGAATCAAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TTGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                                 <  3:200424/148‑1 (MQ=255)                                 tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                     >  3:10943/1‑144 (MQ=255)                                 tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                     <  4:10943/144‑1 (MQ=255)                                   taGGTCCAGCGCTGCTCACCTTCGTCCTTGAGCAGCTTCTGCGACATGCAA‑TCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACTCCGCGATAGGCCt                                                                  <  8:253866/145‑1 (MQ=255)                                      gTCCATCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                     <  1:63111/140‑1 (MQ=255)                                       tCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                    >  2:63111/1‑139 (MQ=255)                                          aGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGAGTTGC‑ATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGa                                                        <  8:125997/148‑1 (MQ=255)                                                ggTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc                                                  >  1:225365/1‑148 (MQ=255)                                                  tCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                >  4:272927/1‑148 (MQ=255)                                                  tCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                <  8:223028/148‑1 (MQ=255)                                                  tCACCTTCGTGCTTGAG‑CAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                >  1:154504/1‑148 (MQ=255)                                                                              gacttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATcc                        <  6:325546/138‑1 (MQ=255)                                                                               acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc                    >  2:194158/7‑148 (MQ=255)                                                                                                caggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACt    <  1:289073/140‑1 (MQ=255)                                                                                                 aggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTg  <  1:194158/142‑1 (MQ=255)                                                                                                  ggcacaGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCT‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTg  >  6:306960/6‑147 (MQ=255)                                                                                                                                      |                                                                                                                CGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACC‑TGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTG  >  NC_002947/4741163‑4741404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑