| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 88.9% | 63.5 / ‑6.0 | 29 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (11/13); minor base A (3/0); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 96.5% | 69.7 / ‑4.4 | 29 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (15/13); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01 | |||||||||||
AGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGG‑CA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTC > NC_002947/1499366‑1499634 || aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑CGCTTTGCCGGTGT‑TCGCGGG‑CA‑cg‑cccgc > 6:79543/1‑144 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCca > 3:238458/1‑148 (MQ=25) gCCATTCAGCGCCGCAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGAt < 7:39828/148‑1 (MQ=21) cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCTCGGG‑CA‑CGCCCGCTCCCACAGACTGATTg < 1:30471/148‑1 (MQ=25) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCGTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCCCAGACTGATTGCCATTAAGCg > 2:20244/1‑148 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACCGGCGCCCCGGTGCCTGGCCCC‑CGCGTTGCCGGTGT‑TGGCGGG‑CC‑CGCCCGCGCCCACAGCCGGGGTGGCCTTAAGCgg > 7:245829/1‑148 (MQ=16) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCg < 5:101100/147‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAATCGGTGGGAGCGGGGGTGCCCGCGAAACAGGCGCCGCTGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 7:74600/148‑1 (MQ=21) aCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCgga < 5:79543/146‑2 (MQ=21) ttGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 2:217022/1‑148 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACC‑CGCATTGCCGGTGT‑TAGCGGGCCC‑CC‑CCCCGCCCCCAGACTGATTGCCAATAAGCCGGCCTATCGCGACAGTg > 5:285251/1‑147 (MQ=18) acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TAGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTTCCAATAAGCCGGGCGATCGCGACAGTGACg > 2:192401/1‑148 (MQ=18) gcaCTGCGGCTCCCAACCGGGGGGAGCGGGCGGCCCCGCTAAACCGGCGCCGCGGGGCA‑GGCCACGGGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg < 6:15710/145‑1 (MQ=21) gAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCCCGAAACAGGCGCCCCCGTGCATGGCACC‑CGCGTTGCCGGTGT‑TAGCCGG‑CACCC‑CCCCGCCCACAGACTGATTGCCATTAAACCGGCCGATCGCTACAGTGACGGCCCGttttt > 6:128226/1‑147 (MQ=255) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGttttt < 7:136130/146‑1 (MQ=21) cTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg‑ttg < 3:293817/148‑1 (MQ=21) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGa < 4:238458/148‑1 (MQ=37) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGAc > 7:148470/1‑148 (MQ=37) cGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACt > 1:178838/1‑148 (MQ=25) tGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACC‑GGCTTGGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTcc < 3:37322/148‑1 (MQ=25) ccGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGCTTGCCTCGCTAACGAATCATGACTCCCt < 1:192401/148‑1 (MQ=25) ataaaCCGGCGCCGCGGT‑CCTTGCCACGGGCTTGGCGGTTGTGC‑GCGGC‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGa < 1:318712/146‑1 (MQ=255) cgccgcGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa < 2:52533/148‑1 (MQ=37) ccgcGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGATTTTTG‑TTGCCTCGCTAACGAATAATGACTCCCTTAGAACGCTATCAAg > 2:126630/1‑148 (MQ=37) gggTGCAGGGCACC‑GGCTTTGCCGTTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGTCTCCCTTAGAACGCTATCATGCAg < 8:49950/147‑1 (MQ=37) tggcacc‑GGCTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 5:224010/7‑146 (MQ=38) cTTTGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGa > 1:239821/1‑148 (MQ=39) tttGCCGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGAGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTcccta > 5:174283/1‑145 (MQ=39) ccGGTGT‑TCGCGGG‑CA‑CGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGATTTTTG‑TTGCCTCGCTAACGAATCATGACTGCCTTAGCACGCTATCAAGCAGATCTGAAAAGTCCCGActtc > 6:151022/1‑147 (MQ=255) || AGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGG‑CA‑CG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTG‑TTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTC > NC_002947/1499366‑1499634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |