| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,740,811 | (T)7→6 | intergenic (+48/+705) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,805 | 0 | T | . | 95.3% | 169.5 / 4.8 | 45 | intergenic (+42/+711) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); major base . (23/18); minor base G (0/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGT > NC_002947/4740659‑4740945 | cACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCttt < 6:107678/149‑1 (MQ=255) gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCttttttat > 2:303745/1‑147 (MQ=255) aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGCAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCttttttatt > 2:184012/1‑146 (MQ=255) aagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCttttttattt < 7:294215/149‑5 (MQ=255) aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCttttttatttgtc < 4:285134/149‑8 (MQ=255) tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg > 4:346805/1‑149 (MQ=255) tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg > 3:273280/1‑149 (MQ=255) tcCAGCCCGTACAAGATCGGCCGTCCGCCCCAGCTGTACACCGGCGAGGAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCCGCC‑TTTTTTATTTGTCCAGTGCCg > 2:48195/1‑149 (MQ=255) tcCAGCCCGTACAAGATCGGACGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg > 5:273465/1‑149 (MQ=255) cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg < 1:184012/147‑1 (MQ=255) cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg < 8:324684/147‑1 (MQ=255) cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc < 7:124710/149‑1 (MQ=255) gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc > 1:290833/1‑149 (MQ=255) gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTc < 3:180036/149‑1 (MQ=255) gTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACCCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctct > 1:295019/1‑147 (MQ=255) aaGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCCGCC‑GTGTTTATTTGTCCTGTGCCGGCCGCTTCGCgg > 7:140929/1‑149 (MQ=255) gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc < 2:77026/149‑1 (MQ=255) ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGc < 4:273280/149‑1 (MQ=255) gCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCTGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGcc < 6:273465/149‑1 (MQ=255) atgCTTTATACCGGCTAGAAGAAGCTCGACATCTTTGCCCTGAAATACCGCAAGTAATCTGCAGTAGCCGAACGCAAGAA‑‑GTCTGGCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAagag < 4:298407/145‑1 (MQ=255) aGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGcc < 3:346805/149‑1 (MQ=255) cTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCACTACCCGAACGCAAGAC‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCgg < 1:88095/149‑1 (MQ=255) cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAACTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCCGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTAAACGGCAATGCACGCCAAGCTGCAGGcaca > 6:123513/1‑148 (MQ=255) cAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACaa > 8:33672/1‑148 (MQ=255) gacgcgcGACAGCGTTGCCCTGAAAGCCCGCAAGTAAGCTGCAGTAGCCTAACGCAAGAC‑‑GGCGGCCCTCGGGCTGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTATGAGGCCGGCACAGACACCACAAAAc < 2:204937/146‑1 (MQ=255) gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCt > 5:100023/1‑148 (MQ=255) cgcgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCa < 7:112242/149‑1 (MQ=255) cgccACTTCGTTGCCCTCAACCCCCCCACGTACGCGCCTGTACCCCACCCCAACAC‑‑GGCTCCCCGGGGGCCG‑CTTTTTTTTTTTGCCCGGTGCCGCCCCCTCCGCGGCCTTGCCCCCTACGCGGCCGGCACAGACACCACAAAACCTCa < 2:290833/149‑1 (MQ=255) gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa > 4:226435/1‑117 (MQ=255) gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa < 3:226435/117‑1 (MQ=255) aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc < 2:294202/119‑1 (MQ=255) tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc > 2:52917/1‑149 (MQ=255) gAAAGACCGCAAGTAAGCTGCAGTAGCCGAATGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGCCACCACAAAACCTCAGTTTTTCTCCGCCCGCt > 1:37761/1‑149 (MQ=255) aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAAGCGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTACGCCCGCTc > 7:182309/1‑149 (MQ=255) cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGCAGAGCCCGGCACCGCCACCACAACACCGCACTTTTTGACCCCCCCCCCACGCAg > 7:196566/1‑149 (MQ=255) aGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCttt > 1:44697/1‑147 (MQ=255) aGCGGCAGTAGCCGAACGCTTGAA‑‑GGCTGCCCTGGGGCTG‑CTTTTTTTATTTGCCCTTGGCCGGCCTCTGCGGGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa < 5:123513/149‑1 (MQ=255) gCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa > 5:14066/1‑84 (MQ=38) gCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa < 6:14066/84‑1 (MQ=38) aGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTAt > 7:242609/1‑149 (MQ=255) ccGAACGCAAGTACCGCCTGCCCCCGGGCAG‑CGTTTTTTATTTGTCCTGGGCCGGCCTGTCCGCGGCTTTCCCCGCCATGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGa < 1:303745/149‑1 (MQ=255) ccGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc > 8:97205/1‑149 (MQ=255) aaCGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc < 7:97205/149‑1 (MQ=255) aCGCAAGAA‑‑GGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCGCACGCAGCCCTGTCAGGGTATTGAACGgcg > 3:286848/1‑149 (MQ=255) aagaa‑‑gGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc < 6:100023/149‑1 (MQ=255) gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac < 3:118388/149‑1 (MQ=255) tGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCGGGCACAGCCACCCAAAAACCGCAGTTTTTTTCCGCCCGCGCACGCAGCCCTTTAAGGGTTTTGAACCGCGCATCCCCCCCg > 3:204024/1‑148 (MQ=255) ccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAG‑‑TTTCTCAGCCCGCTCACGCAGCCCGTTAAGGGTATTGAAAGGCGCATCCACCACGAACttg > 3:285664/1‑149 (MQ=255) cgagggCAGCC‑TTTTTTATTTGACGTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACtt < 7:39156/146‑1 (MQ=255) cGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCCTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgt > 5:210491/1‑149 (MQ=255) | CACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAA‑‑GGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGT > NC_002947/4740659‑4740945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |