| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,508 | +C | intergenic (+85/‑79) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,506 | 1 | . | C | 96.3% | 67.1 / ‑4.6 | 27 | intergenic (+83/‑81) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (13/13); minor base G (0/1); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAC > NC_002947/1499360‑1499643 | cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑‑c < 6:135742/149‑1 (MQ=37) gAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGGCGGTGTTAGCGGGCAcg‑‑cccgc > 5:88309/1‑146 (MQ=37) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGCGGAAAAGGCGCCGCGGGGGATGGCACCGGCTTTGCCGGTGTTAGCGGGCAcg‑‑cccgct > 4:87412/1‑145 (MQ=25) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑‑cccgct < 1:88116/149‑5 (MQ=25) aGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAGCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCAcg‑‑cccgctc < 3:87412/149‑6 (MQ=25) cccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTccc < 4:255970/149‑1 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCca > 4:210914/1‑148 (MQ=25) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTAGCGGGCAAGC‑CCGCGACCACAGAATGAtt > 6:250474/1‑149 (MQ=21) cATTCAGAGCCTTAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGACACAGGCGCCGCGGAGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGAtttc < 5:250474/149‑3 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCg > 6:143540/1‑149 (MQ=21) cTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCgg < 5:143540/149‑1 (MQ=21) tGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGTGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCggg > 1:14590/1‑149 (MQ=21) cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCt > 6:338057/1‑148 (MQ=21) gCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt > 4:280230/1‑149 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCGCGGTGCATGGCACCCGCGTTGCCCGTGTTAGCGGGCCCGC‑CCCCGCCCACAGCCTGATTGCCAATAAGCGGGCCAATCGCGACAGTGa > 8:87773/1‑149 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa < 3:210914/149‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 7:87773/147‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 1:8159/147‑1 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgtt < 5:273836/149‑1 (MQ=21) tcttccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGTGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 1:35597/145‑1 (MQ=21) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCTAAACCGGCGCCGCGGGGAATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTAc < 4:296436/127‑1 (MQ=18) gCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCCCGC‑CCGCGCCCCCACACTGATTGCCATTAAGCGGGCCTATCGCGAc > 3:296436/1‑127 (MQ=17) aaaCGGTGGGAGCGGGCCTGCCCGCGAAACAGGCGCCGCGGGGCATGGCACCGGCATTGCCGGTGTTCGCGGGCCCGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCGATCGCGACAGTGACGGCCCGTTTTTTGTTTCCTCGc > 5:132405/1‑149 (MQ=21) tcGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 5:262165/148‑1 (MQ=25) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 5:338057/149‑1 (MQ=25) gAAACAGGCGCCGCGGGGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAAc < 6:3515/149‑1 (MQ=37) cggggccgcGGTGAATGGAACCGGCTTTGCGGTTGTT‑GGGGGCAAGGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt < 1:332163/145‑1 (MQ=255) aGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTAt > 7:310062/1‑149 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc > 2:69758/1‑148 (MQ=37) gcgcCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCa < 6:88309/149‑1 (MQ=37) tGCCGGTGTTCGCGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATCACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActt > 5:247454/1‑148 (MQ=255) tcgcGGGCACGC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc > 7:307547/4‑149 (MQ=255) | CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑TCGGGCACG‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAC > NC_002947/1499360‑1499643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |