| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 2,921,460 | G→A | G3494R (GGG→AGG) | PP_2561 → | hemolysin‑type calcium‑binding bacteriocin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,921,460 | 0 | G | A | 95.7% | 145.2 / 0.1 | 47 | coding (10480/10827 nt) | PP_2561 | hemolysin‑type calcium‑binding bacteriocin |
| Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (23/21); minor base . (0/2); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑GGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921331‑2921600 | gCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACtt < 3:101900/149‑1 (MQ=255) gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCt > 5:131314/1‑148 (MQ=255) tAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTc > 7:203883/1‑148 (MQ=255) gACAACCTGAACGGGGGAGCTGGCGATGACAGCCTGGATGGCGGCAACCGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGAGACGAGCGTACCGACACCCTCATC‑AGGGGGGCCGGCAACGACTTCCTCAACgg > 1:228145/1‑149 (MQ=255) tctaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACg < 5:272350/144‑1 (MQ=255) cAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACCGCAATGACACCCTGCAGGGCGCCGATGCTAACGAAACATTGTTCGGCGACGTGGGTACCGACCCCCTCATC‑AGGGCGGCCGGCAACGACTTCCTCAACggag > 4:266484/1‑147 (MQ=255) aaCCTGACCGCGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGc < 5:155179/149‑1 (MQ=255) ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACg > 6:272350/1‑143 (MQ=255) cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑‑AGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCa < 5:34067/149‑1 (MQ=255) gAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAg > 2:250277/1‑149 (MQ=255) aaCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAgg > 3:160409/1‑149 (MQ=255) ggCGCTGGAGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa < 2:228145/149‑1 (MQ=255) gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACgg‑tg < 8:203883/149‑1 (MQ=255) tGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑tggtgg < 6:131314/149‑1 (MQ=255) cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑Tggtgggt < 3:266484/148‑1 (MQ=255) aGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGgtgggtgg > 8:175500/1‑149 (MQ=255) aGCCGGGATGGCGGCAACGGCAATGACACCCTTCAGGGGGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGTTCAGGGGGGCCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑Tggtgggt < 1:265894/148‑1 (MQ=255) ccTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTgggg > 4:9907/1‑149 (MQ=255) cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGc < 5:164658/149‑1 (MQ=255) aacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAgg < 5:227117/113‑1 (MQ=255) aacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAgg > 6:227117/1‑113 (MQ=255) gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCAt > 8:291213/1‑148 (MQ=255) aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGgtgggtgg > 3:2874/1‑128 (MQ=255) aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGgtgggtgg < 4:2874/128‑1 (MQ=255) aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGAt > 3:172569/1‑149 (MQ=255) tGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt > 2:349223/1‑149 (MQ=255) ggcggcGATGGTAACGCAAAATTGTTCGGCGAAGGGGGTACCGACAACCTGATC‑ACGGGGGCCGGCAAAGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTTGGTGGGTGGGGCCGGTAACGACACCa < 1:15032/131‑1 (MQ=255) ggcggcGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCa > 2:15032/1‑130 (MQ=255) ggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa > 3:1488/1‑149 (MQ=255) gTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTc < 4:160409/149‑1 (MQ=255) aCGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACTACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGCTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa < 1:250277/148‑1 (MQ=255) acacATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTc < 7:175500/149‑1 (MQ=255) ttGTTCGGCGACGTTGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGc < 8:220825/149‑1 (MQ=255) ggCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCtt < 1:349223/149‑1 (MQ=255) gCGACGTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt < 3:9907/149‑1 (MQ=255) gTGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCaa > 2:117889/1‑149 (MQ=255) gTGGGTACCGACACCCTGATC‑AGCGGGGCCGGCAACGACTTCCTCAACGGTGCGGCACGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCaa > 1:106330/1‑149 (MQ=255) tGGGTACCGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc < 4:172569/149‑1 (MQ=255) tACCGACACCCTGATC‑‑AGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCt < 2:700/149‑1 (MQ=255) cGACACCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGa > 2:78312/1‑149 (MQ=255) acCCTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat < 1:117889/149‑1 (MQ=255) ccTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt > 6:87450/1‑130 (MQ=255) ccTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt < 5:87450/130‑1 (MQ=255) ccTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc > 5:329726/1‑148 (MQ=255) ccTGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGACACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcata > 7:72974/1‑147 (MQ=255) tGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatc < 7:56997/143‑1 (MQ=255) tGATC‑AGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatc > 8:56997/1‑143 (MQ=255) | GCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑GGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC > NC_002947/2921331‑2921600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |