Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,674,753	G→C	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	245.0 / NA	71	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (36/35);  total (36/35)

CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674613‑5674888                                                                                                                                              |                                                                                                                                          cAGCGCTGCTGACTCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCgg                                                                                                                                    <  1:124942/149‑1 (MQ=255)   gcgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                  <  3:166037/149‑1 (MQ=255)   gcgaTGCTGACCAGCAATGGCTGATTGATGTACCGAGTGTGCTGCTTCGCAGCCCTATAACGACACAAGTCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCATTGTGTCGTTATAGGGCCGCAAAGCGGCCCCCTCGGtt                                                                                                                                  <  8:61446/149‑1 (MQ=255)        gctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAg                                                                                                                             >  2:207436/1‑149 (MQ=255)           gACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTg                                                                                                                          >  4:258874/1‑149 (MQ=255)            aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                           <  5:110314/147‑1 (MQ=255)              ccGCAAGGGCTGATAGAAGTCCCGAGGGGGCTGCTTCGCAGCCCTATCACGACCCAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCa                                                                                                                       <  6:184470/149‑1 (MQ=37)               cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTgg                                                                                                                         >  4:219547/1‑146 (MQ=255)                gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  1:8466/1‑148 (MQ=255)                gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  1:243616/1‑148 (MQ=255)                gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGGTTTCAGCTGGCAg                                                                                                                      >  4:244578/1‑148 (MQ=255)                  aaGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGaaa                                                                                                                   <  2:307240/149‑1 (MQ=255)                          aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTc                                                                                                            <  3:258874/148‑1 (MQ=255)                              aaGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCt                                                                                                        >  4:118349/1‑148 (MQ=255)                               aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc                                                                                                      <  4:273104/149‑1 (MQ=255)                                     gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCt                                                                                                <  3:118349/149‑1 (MQ=255)                                       gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                              <  7:342040/149‑1 (MQ=255)                                         gggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                            <  6:7059/149‑1 (MQ=255)                                          ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                            >  3:100099/1‑148 (MQ=255)                                              gctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑Tg                                                                                      <  2:243616/149‑1 (MQ=255)                                               ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGa                                                                                     >  5:269669/1‑149 (MQ=255)                                                ttCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGa                                                                                     >  1:146396/1‑148 (MQ=255)                                                  cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                          >  6:215920/1‑142 (MQ=255)                                                  cGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGaca                                                                                          <  5:215920/142‑1 (MQ=255)                                                   gCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtc                                                                                 >  7:69648/1‑149 (MQ=255)                                                    cAGCTCTATCACGACAGTAGGCCTGTCCTGCAGAGAATTTCTTTAGATCTACATTTGCTGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtc                                                                                 <  3:21681/148‑1 (MQ=255)                                                     aGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtcca                                                                               <  3:219547/149‑1 (MQ=255)                                                         cTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtccaggtc                                                                           <  4:120555/149‑1 (MQ=255)                                                           aTCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtccaggtc                                                                           >  5:144189/1‑147 (MQ=255)                                                            tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGCCAC‑TGATAtccaggtccag                                                                        >  6:122024/1‑149 (MQ=255)                                                            tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtccaggtccag                                                                        <  2:146396/149‑1 (MQ=255)                                                             cACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtccaggtccagg                                                                       >  6:35901/1‑149 (MQ=255)                                                             cACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAtccaggtccagg                                                                       >  2:117317/1‑149 (MQ=255)                                                               cGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑t                                                                    >  4:165098/1‑149 (MQ=255)                                                                    caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCg                                                               <  2:92961/149‑1 (MQ=255)                                                                     aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACtt                                                                                                             <  7:25973/104‑1 (MQ=255)                                                                     aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACtt                                                                                                             >  8:25973/1‑104 (MQ=255)                                                                     aaGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTACAGGG‑TCTGCg                                                               >  8:135492/1‑148 (MQ=255)                                                                        gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                           <  6:227805/87‑1 (MQ=25)                                                                        gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCt                                                                                                                           >  5:227805/1‑87 (MQ=25)                                                                        gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGt                                                           <  5:119096/149‑1 (MQ=255)                                                                             tCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATAGCCAGGTCCCCTG‑TCTGCGAAGTATCAc                                                      >  8:159520/1‑149 (MQ=38)                                                                                tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCg                                                   >  3:275351/1‑149 (MQ=255)                                                                                 gCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCgg                                                  >  8:68430/1‑149 (MQ=255)                                                                                  cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCggg                                                 >  5:345289/1‑149 (MQ=255)                                                                                   agagAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGc                                                <  8:69648/149‑1 (MQ=255)                                                                                       aaTTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCttt                                            <  6:345289/149‑1 (MQ=255)                                                                                         tttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCt                                                                                                >  8:132246/1‑97 (MQ=255)                                                                                         tttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCt                                                                                                <  7:132246/97‑1 (MQ=255)                                                                                          ttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGAt                                         >  5:349143/1‑149 (MQ=255)                                                                                           tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGAt                                         <  6:269669/148‑1 (MQ=255)                                                                                            cTTGATCTCTACAGGCGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATg                                        <  6:24832/148‑1 (MQ=255)                                                                                               gctctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                     <  5:35901/146‑1 (MQ=255)                                                                                                 tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  1:207436/148‑1 (MQ=255)                                                                                                     tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  3:165098/144‑1 (MQ=255)                                                                                                     tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                   <  1:324639/144‑1 (MQ=255)                                                                                                     caCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgt                              >  8:277904/2‑149 (MQ=255)                                                                                                          gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATg                                        <  2:236230/134‑1 (MQ=255)                                                                                                          gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         >  4:257456/1‑149 (MQ=255)                                                                                                          gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         >  3:193524/1‑149 (MQ=255)                                                                                                          gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcagg                         >  3:146502/1‑149 (MQ=255)                                                                                                                       tCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg             <  4:275351/148‑1 (MQ=255)                                                                                                                       tCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtg             <  1:117317/148‑1 (MQ=255)                                                                                                                         gTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          >  6:263217/1‑149 (MQ=255)                                                                                                                         gTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg          >  4:332101/1‑149 (MQ=255)                                                                                                                          tGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACATGA‑ATCCAGGTCCAGGGAGATGGGAAGTTGCCCCCCGGCCATTTATTCCCAATTGGTTAAGGGTAAGCgtggtgggg          >  1:112408/1‑147 (MQ=255)                                                                                                                          tGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCTGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGtggtggtgg         >  3:120636/1‑149 (MQ=255)                                                                                                                           gATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGc        >  3:120188/1‑149 (MQ=255)                                                                                                                             tAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt      <  7:68430/149‑1 (MQ=255)                                                                                                                                 gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa  <  6:144189/149‑1 (MQ=255)                                                                                                                                 gCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCATCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg    >  3:106054/1‑147 (MQ=255)                                                                                                                                              |                                                                                                                                          CAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC‑TGATATCCAGGTCCAGGG‑TCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAA  >  NC_002947/5674613‑5674888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑