mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-69_S69_L001_R1_001.good.fq | 362,338 | 51,634,607 | 100.0% | 142.5 bases | 149 bases | 94.1% |
| errors | ALE-69_S69_L001_R2_001.good.fq | 362,338 | 51,430,287 | 100.0% | 141.9 bases | 149 bases | 94.6% |
| errors | ALE-69_S69_L002_R1_001.good.fq | 355,764 | 50,594,663 | 100.0% | 142.2 bases | 149 bases | 95.3% |
| errors | ALE-69_S69_L002_R2_001.good.fq | 355,764 | 50,442,827 | 100.0% | 141.8 bases | 149 bases | 95.1% |
| errors | ALE-69_S69_L003_R1_001.good.fq | 369,662 | 52,550,544 | 100.0% | 142.2 bases | 149 bases | 95.2% |
| errors | ALE-69_S69_L003_R2_001.good.fq | 369,662 | 52,369,414 | 100.0% | 141.7 bases | 149 bases | 95.6% |
| errors | ALE-69_S69_L004_R1_001.good.fq | 342,091 | 48,636,689 | 100.0% | 142.2 bases | 149 bases | 95.1% |
| errors | ALE-69_S69_L004_R2_001.good.fq | 342,091 | 48,484,570 | 100.0% | 141.7 bases | 149 bases | 94.7% |
| total | 2,859,710 | 406,143,601 | 100.0% | 142.0 bases | 149 bases | 95.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 61.8 | 2.3 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 52239 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 604 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.030 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.81695 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 17:17:23 17 Feb 2020 | 17:18:05 17 Feb 2020 | 42 seconds |
| Read alignment to reference genome | 17:18:05 17 Feb 2020 | 17:24:14 17 Feb 2020 | 6 minutes 9 seconds |
| Preprocessing alignments for candidate junction identification | 17:24:14 17 Feb 2020 | 17:24:54 17 Feb 2020 | 40 seconds |
| Preliminary analysis of coverage distribution | 17:24:54 17 Feb 2020 | 17:26:31 17 Feb 2020 | 1 minute 37 seconds |
| Identifying junction candidates | 17:26:31 17 Feb 2020 | 17:31:50 17 Feb 2020 | 5 minutes 19 seconds |
| Re-alignment to junction candidates | 17:31:50 17 Feb 2020 | 17:33:49 17 Feb 2020 | 1 minute 59 seconds |
| Resolving best read alignments | 17:33:49 17 Feb 2020 | 17:34:51 17 Feb 2020 | 1 minute 2 seconds |
| Creating BAM files | 17:34:51 17 Feb 2020 | 17:36:20 17 Feb 2020 | 1 minute 29 seconds |
| Tabulating error counts | 17:36:20 17 Feb 2020 | 17:36:50 17 Feb 2020 | 30 seconds |
| Re-calibrating base error rates | 17:36:50 17 Feb 2020 | 17:36:52 17 Feb 2020 | 2 seconds |
| Examining read alignment evidence | 17:36:52 17 Feb 2020 | 17:42:35 17 Feb 2020 | 5 minutes 43 seconds |
| Polymorphism statistics | 17:42:35 17 Feb 2020 | 17:42:36 17 Feb 2020 | 1 second |
| Output | 17:42:36 17 Feb 2020 | 17:43:15 17 Feb 2020 | 39 seconds |
| Total | 25 minutes 52 seconds | ||
