Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,262,544 Δ1 bp 5.4% coding (717/1488 nt) PP_4638 → methylenetetrahydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,262,5440C. 5.4% 240.7 / 7.0 74coding (717/1488 nt)PP_4638methylenetetrahydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (31/39);  new base . (2/2);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

CTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAA  >  NC_002947/5262397‑5262692
                                                                                                                                                   |                                                                                                                                                    
cTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTcc                                                                                                                                                      >  7:73509/1‑148 (MQ=255)
 tGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTcc                                                                                                                                                      <  5:129688/147‑1 (MQ=255)
 tGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTcc                                                                                                                                                      >  6:129688/1‑147 (MQ=255)
   gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAgg                                                                                                                                                  >  1:1173/1‑149 (MQ=255)
   gATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAgg                                                                                                                                                  >  3:307816/1‑149 (MQ=255)
        gcCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCttt                                                                                                                                             <  4:307816/149‑1 (MQ=255)
           gTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGcc                                                                                                                                          <  8:54292/149‑1 (MQ=255)
                gCCTCACCCAAGCCCATGCTGGCCTGCCTCTTGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc                                                                                                                                     >  1:26914/1‑149 (MQ=255)
                gCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgc                                                                                                                                     >  4:157465/1‑149 (MQ=255)
                  cTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgc                                                                                                                                   >  1:166483/1‑149 (MQ=255)
                     aCCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGgcgcg                                                                                                                                  <  3:157465/147‑1 (MQ=255)
                       ccAAGCCCATGCTGGCCTGCCTCATGAGCCTCCCCCACGGGCGCGCGACGATGCTCGACCCAGTGGCCGGCGTGACTGTCACGCCCTCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAgt                                                                                                                              >  5:13034/1‑149 (MQ=255)
                         aaGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAgtgt                                                                                                                            >  2:82293/1‑149 (MQ=255)
                          aGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGtt                                                                                                                           <  8:140334/149‑1 (MQ=255)
                                tGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGAGGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGc                                                                                                                     <  4:37772/149‑1 (MQ=255)
                                  cTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCt                                                                                                                   <  5:104836/149‑1 (MQ=255)
                                         gcctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTg                                                                                                            >  8:140291/1‑149 (MQ=255)
                                          cctCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTg                                                                                                            >  8:275376/1‑148 (MQ=255)
                                                   ccTCACCCACGGGCGCTCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCg                                                                                                  <  7:70476/149‑1 (MQ=255)
                                                   ccTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCg                                                                                                  <  7:275376/149‑1 (MQ=255)
                                                   ccTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCg                                                                                                  <  3:292486/149‑1 (MQ=255)
                                                   ccTAACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTAACGCCAACCATTCGCGACATGCTAGAAGCGGAGACGGCGCAGTCCAAGGCCTTTTCCCAGGCGCGCAGAGTTTCCCGCCAGGCCTTGCAGATTAtac                                                                                                  >  8:257122/1‑147 (MQ=255)
                                                       aCCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGt                                                                                                <  1:125623/147‑1 (MQ=255)
                                                         ccACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACa                                                                                             >  4:193301/1‑148 (MQ=255)
                                                                cgcgcgACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGAt                                                                                     <  7:192519/149‑1 (MQ=255)
                                                                 gcgcgACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTTCAAAGCTGATg                                                                                    >  8:178172/1‑149 (MQ=255)
                                                                 gcgcgACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATg                                                                                    <  4:44631/149‑1 (MQ=255)
                                                                  cgcgACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATgg                                                                                   <  2:26914/149‑1 (MQ=255)
                                                                  cgcgACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATgg                                                                                   <  2:1173/149‑1 (MQ=255)
                                                                        gaTGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCtt                                                                                                             <  4:68344/117‑1 (MQ=255)
                                                                        gaTGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCtt                                                                                                             >  3:68344/1‑117 (MQ=255)
                                                                           gCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGCCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATGGGTCCTAAGCTGATGGGCTACGCCg                                                                          >  5:178728/1‑149 (MQ=255)
                                                                            cTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCgg                                                                         >  7:65107/1‑149 (MQ=255)
                                                                               gACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGgt                                                                        <  2:92589/147‑1 (MQ=255)
                                                                                 ccACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGtgtg                                                                     >  4:155746/1‑148 (MQ=255)
                                                                                 ccACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGT‑‑‑AGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCAcc                                                                 >  6:131589/1‑149 (MQ=255)
                                                                                 ccACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGT‑‑‑AGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCAcc                                                                 >  2:232595/1‑149 (MQ=255)
                                                                                    cGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCAcc                                                                 >  5:53790/1‑149 (MQ=255)
                                                                                     gTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCt                                                                <  8:65107/149‑1 (MQ=255)
                                                                                      tggatggCGTGACAGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTg                                                               >  7:30714/6‑149 (MQ=255)
                                                                                       ggCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATggg                                                                                  <  1:192682/129‑1 (MQ=255)
                                                                                       ggCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATggg                                                                                  >  2:192682/1‑129 (MQ=255)
                                                                                          cGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAg                                                           <  6:145813/149‑1 (MQ=255)
                                                                                            gCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAggg                                                         <  7:140291/149‑1 (MQ=255)
                                                                                               tGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGtt                                                      <  3:155746/149‑1 (MQ=255)
                                                                                                gACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGtt                                                      >  2:37871/1‑148 (MQ=255)
                                                                                                gACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTc                                                     <  8:73509/149‑1 (MQ=255)
                                                                                                 aCTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTc                                                     <  3:193301/148‑1 (MQ=255)
                                                                                                  cTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTcac                                                   <  3:110032/149‑1 (MQ=255)
                                                                                                   tGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTcaca                                                  >  5:294662/1‑149 (MQ=255)
                                                                                                       aCGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGc                                               <  2:166483/148‑1 (MQ=255)
                                                                                                       aCGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCt                                              >  6:14259/1‑149 (MQ=255)
                                                                                                        cGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCt                                                                <  6:323221/130‑1 (MQ=255)
                                                                                                        cGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCt                                                                >  5:323221/1‑130 (MQ=255)
                                                                                                        cGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTc                                             <  1:82293/149‑1 (MQ=255)
                                                                                                         gCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTc                                             >  3:145936/1‑148 (MQ=255)
                                                                                                          ccatccatGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCg                                            >  1:6342/1‑148 (MQ=255)
                                                                                                           catccatGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGaa                                          >  8:244374/1‑149 (MQ=255)
                                                                                                            atccatGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGaa                                          >  3:296099/1‑148 (MQ=255)
                                                                                                               catGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAg                                       <  7:257122/148‑1 (MQ=255)
                                                                                                                      gACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGc                               <  3:10666/149‑1 (MQ=255)
                                                                                                                         aTGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCt                            <  6:294662/149‑1 (MQ=255)
                                                                                                                            cTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGga                         <  5:181433/149‑1 (MQ=255)
                                                                                                                               gAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGgaga                       <  4:145936/148‑1 (MQ=255)
                                                                                                                               gAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGAc                      >  4:108099/1‑149 (MQ=255)
                                                                                                                                 aGCGGCGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCGGGCCGTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGGTCACACGCTCGAACAGCTGCTGGCCCTGGAGAccc                    <  6:13034/149‑1 (MQ=255)
                                                                                                                                     gagaCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTAt                >  7:123770/1‑149 (MQ=255)
                                                                                                                                     gagaCGGCGCAGT‑‑‑AGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGa             <  1:232595/149‑1 (MQ=255)
                                                                                                                                     gagaCGGCGCAGT‑‑‑AGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGa             <  5:131589/149‑1 (MQ=255)
                                                                                                                                      agaCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATc               <  3:108099/149‑1 (MQ=255)
                                                                                                                                       gaCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATc               >  1:165209/1‑148 (MQ=255)
                                                                                                                                        aCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGa             <  6:53790/149‑1 (MQ=255)
                                                                                                                                        aCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGa             <  2:65206/149‑1 (MQ=255)
                                                                                                                                        aCGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGa             <  5:14259/149‑1 (MQ=255)
                                                                                                                                            cgcAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTAc         <  8:30714/149‑1 (MQ=255)
                                                                                                                                               aGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTgg      >  1:63658/1‑149 (MQ=255)
                                                                                                                                                gTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGc     <  2:63658/149‑1 (MQ=255)
                                                                                                                                                   cAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCaaa  >  2:117517/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGGATGCGCCGTCAGGCCTCACCCAAGCCCATGCTGGCCTGCCTCATGAGCCTCACCCACGGGCGCGCGACGATGCTCGACCACGTGGCCGGCGTGACTGTCACGCCATCCATGCGCGACATGCTCGAAGCGGAGACGGCGCAGTCCAAGGCCTTTGCCCAGGCGCGCAGTGTTGACCGCCTGGCCTTGCAGATTATCGGTACAAAGCTGATGGGCTACGCCGGTGTGCACCTGTCAGGGGTTCACACGCTCGAACAGCTGCTGGCCCTGGAGACCCGTATCGAGTACTGGCAAA  >  NC_002947/5262397‑5262692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑