Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,424,490 Δ1 bp 8.0% coding (89/915 nt) PP_4764 → histone deacetylase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,424,4900A. 8.0% 149.3 / 7.9 50coding (89/915 nt)PP_4764histone deacetylase family protein
Reads supporting (aligned to +/- strand):  ref base A (28/18);  new base . (2/2);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATTGCAGTCACCCGTCATGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAGGACCAGCGCC  >  NC_002947/5424345‑5424636
                                                                                                                                                 |                                                                                                                                                   
ccATTGCAGTCACCCGTCATGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCAGATCTACCACGAAGATTACAGCCCGCAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGa                                                                                                                                                  >  5:78089/1‑149 (MQ=255)
  aTTGCAGTCACCCGTCATGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCACGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGacc                                                                                                                                                >  8:303150/1‑149 (MQ=255)
       aGTCACGCGTCATGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACGACGTAGATTACAGCCCGGATTTCACCGCTGAACACCGCTTCCCGATGTACAAGTTCCGCCTTCTGCACGACCACCt                                                                                                                                            <  1:298636/148‑1 (MQ=255)
                tCATGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGc                                                                                                                                 >  7:240705/1‑149 (MQ=255)
                   tGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCggg                                                                                                                              >  7:72528/1‑149 (MQ=255)
                   tGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCCGCTGC‑CGACCAGGTGGTCGTGCAGCagg                                                                                                                              <  8:55527/149‑1 (MQ=255)
                        aGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGTAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGac                                                                                                                         <  1:19705/149‑1 (MQ=255)
                        aGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGac                                                                                                                         <  2:63643/149‑1 (MQ=255)
                         gATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGccc                                                                                                                        >  8:178284/1‑149 (MQ=255)
                             aGCACTGGCCCTGATTGCGACCCCATCCATGACGTTGCCGCTGAGCTCCCACGAAGATTACAGCCCGGAGTGCCCCGCTGAACACCGCTTCCCGATGGACACGTTACGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCg                                                                                                                    >  6:329099/1‑149 (MQ=255)
                                          aTTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCctgct                                                                                                       <  7:303150/149‑1 (MQ=255)
                                                 ccccATCCATGCGGTTGCCGCTGATCTACCACGACGATTACCGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCGTGCTGCGCCCg                                                                                                <  6:78089/149‑1 (MQ=255)
                                                 ccccATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCctgctg                                                                                                      >  4:55956/1‑143 (MQ=255)
                                                 ccccATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCctgctg                                                                                                      <  3:55956/143‑1 (MQ=255)
                                                 ccccATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCg                                                                                                <  8:72528/149‑1 (MQ=255)
                                                         atGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTACACGCTGAACACCTCTTCCCTATGCACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACAAGGCCCTCCTGCGCCCGGACATCTg                                                                                        >  8:101698/1‑149 (MQ=255)
                                                                           tACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGCCCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGCCATCCTCGc                                                                      >  2:318328/1‑149 (MQ=255)
                                                                           tACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGc                                                                      >  6:134375/1‑149 (MQ=255)
                                                                            aCCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGcc                                                                     >  5:262328/1‑149 (MQ=255)
                                                                                       tACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGATGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGa                                                          >  5:305482/1‑149 (MQ=255)
                                                                                          aGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCg                                                       >  4:227231/1‑149 (MQ=255)
                                                                                               ggAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGc                                                   >  7:262493/1‑148 (MQ=255)
                                                                                                   ttCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACAt                                              >  6:297255/1‑149 (MQ=255)
                                                                                                     ccccGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATc                                             <  3:227231/148‑1 (MQ=255)
                                                                                                      cccGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCCACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATc                                             >  7:231575/1‑147 (MQ=255)
                                                                                                       ccGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGc                                                   <  4:234015/140‑1 (MQ=255)
                                                                                                       ccGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGc                                                   >  3:234015/1‑140 (MQ=255)
                                                                                                        cGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAgc                                         <  1:318328/149‑1 (MQ=255)
                                                                                                                     ttCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCg                                            <  3:283132/133‑1 (MQ=255)
                                                                                                                     ttCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCg                                            >  4:283132/1‑133 (MQ=255)
                                                                                                                        ccGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGa                         <  8:240705/149‑1 (MQ=255)
                                                                                                                         cGATGGACAAGTTCCGCCAGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAg                        >  2:264438/1‑149 (MQ=255)
                                                                                                                              gACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTc                   >  1:186147/1‑149 (MQ=255)
                                                                                                                                cAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGaa                               <  1:8309/135‑1 (MQ=255)
                                                                                                                                cAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGaa                               >  2:8309/1‑135 (MQ=255)
                                                                                                                                cAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTcgc                 <  7:178284/149‑1 (MQ=255)
                                                                                                                                 aaGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGCCTGACCACCGACCACGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCCCCCTGGCCCATGACCGCAGCTACATCCAGCCCGACATGAACGGCGAGCTGTcgcg                >  8:51732/1‑149 (MQ=255)
                                                                                                                                   gTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTcgcgcg              <  5:297255/149‑1 (MQ=255)
                                                                                                                                   gTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATCAACGGCGAGCTGTcgcgc               >  4:306935/1‑148 (MQ=255)
                                                                                                                                   gTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGCGCTGACCACCGACCAGGACCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCAAGACCCCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTcgagc               >  6:46582/1‑148 (MQ=255)
                                                                                                                                    ttCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTcgcgc               >  6:277998/1‑147 (MQ=255)
                                                                                                                                      ccGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAg            >  2:187361/1‑148 (MQ=255)
                                                                                                                                       cGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAgg           <  5:277998/148‑1 (MQ=255)
                                                                                                                                        gCCCGCTGC‑CGACCAGGTGGTCGTGCAGCAGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCt                      >  5:69501/1‑136 (MQ=38)
                                                                                                                                        gCCCGCTGC‑CGACCAGGTGGTCGTGCAGCAGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCt                      <  6:69501/136‑1 (MQ=38)
                                                                                                                                        gCCCGCTGC‑CGACCAGGTGGTCGTGCAGCAGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAGGAc         >  3:149048/1‑149 (MQ=255)
                                                                                                                                          ctgctgCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAGGAc         >  3:247893/1‑147 (MQ=255)
                                                                                                                                           tgctgcACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTcgc                 >  5:306892/1‑138 (MQ=255)
                                                                                                                                           tgctgcACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTcgc                 <  6:306892/138‑1 (MQ=255)
                                                                                                                                               gcACGACCACCTTGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAGGACCAGcgcc  <  6:152981/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCATTGCAGTCACCCGTCATGGCAAGATCAGCACTGGCCCTGATTGCGACCCCATCCATGCCGTTGCCGCTGATCTACCACGAAGATTACAGCCCGGAGTTCCCCGCTGAACACCGCTTCCCGATGGACAAGTTCCGCCTGCTGCACGACCACCTGGTCG‑GCAGCGGGCTGACCACCGACCAGGCCCTGCTGCGCCCGGACATCTGCCCCAACGACATCCTCGCCCTGGCCCATGACCGCAGCTACATCGAGCGCTACATGAACGGCGAGCTGTCGCGCGAGGACCAGCGCC  >  NC_002947/5424345‑5424636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑