Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,458,898 Δ1 bp 5.1% intergenic (+23/‑39) leuS → / → PP_4795 leucine‑‑tRNA ligase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,458,8980T. 5.1% 240.6 / 6.8 79intergenic (+23/‑39)leuS/PP_4795leucine‑‑tRNA ligase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (35/39);  new base . (2/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

CCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGACGT  >  NC_002947/5458755‑5459046
                                                                                                                                                |                                                                                                                                                       
ccGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCgg                                                                                                                                                    <  1:216399/149‑1 (MQ=255)
ccGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCgg                                                                                                                                                    <  4:313399/149‑1 (MQ=255)
    gaagaaGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCAATCGATGCGGGTgg                                                                                                                                                <  3:120604/149‑1 (MQ=255)
         agTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑gg                                                                                                                                         >  1:40960/1‑148 (MQ=255)
             gAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAg                                                                                                                                    >  4:315926/1‑149 (MQ=255)
             gAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAg                                                                                                                                    >  5:173109/1‑149 (MQ=255)
             gAAGACGCTGCCCGCAGCAACGAGAATGTCCTGCGATTCATCGATTGCCTGACCAACAGCAAGGTCATCGTGGTACCGTGCAAGCTGTTCAACATCGTCTCCAACTGATGAAAACGCCCACCCCCATCGAATGCGGGTGGGC‑‑‑GGAACa                                                                                                                                     <  5:86916/148‑1 (MQ=255)
               aGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGc                                                                                                                                  <  1:62176/149‑1 (MQ=255)
                gCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGcc                                                                                                                                 <  2:40960/149‑1 (MQ=255)
                   gCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCa                                                                                                                               >  5:97969/1‑148 (MQ=255)
                       cccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCCTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGCCCCaaaacg                                                                                                                          >  7:23648/1‑147 (MQ=255)
                        ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAggg                                                                                                                         <  5:329909/149‑1 (MQ=255)
                        ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAgg                                                                                                                          >  7:53225/1‑148 (MQ=255)
                        ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAgg                                                                                                                          >  2:306876/1‑148 (MQ=255)
                         cGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAgg                                                                                                                          >  7:196884/1‑147 (MQ=255)
                         cGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGa                                                                                                                        <  7:268818/149‑1 (MQ=255)
                          gcagcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCaaaa                                                                                                                            <  7:133478/144‑1 (MQ=255)
                          gcagcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCaaaa                                                                                                                            >  8:133478/1‑144 (MQ=255)
                            agcaACGAGAATTTCCTGGGCTTCTTCGATGGCCTGACCATCCGCAATGTCATCGTGTTACCGGGCAAGCTGGTAAACATCGTCGCCAACTGATGAAAACGCCCACCGGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAg                                                                                                                       <  8:17572/147‑1 (MQ=255)
                                aCGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGcaacaa                                                                                                                 >  3:186114/1‑149 (MQ=255)
                                   agaATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACAt                                                                                                              <  8:53225/149‑1 (MQ=255)
                                    gaATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGCGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATg                                                                                                             <  8:230172/149‑1 (MQ=255)
                                                    aTCGATGGCCTGACCATCCGCAATGTCATCGTTGTACAGGGCAAGCTGGGCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGTC‑‑‑GGAACAGGCCCAAAAGGTAGCAACAACATGATCAAACGCAATTTGc                                                                                             <  2:32060/149‑1 (MQ=255)
                                                    aTCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGc                                                                                             >  8:61150/1‑149 (MQ=255)
                                                    aTCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACAGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCACAAAGTGAGCAACAAGATGATCAAACGCAATTTGc                                                                                             >  8:264233/1‑149 (MQ=255)
                                                     tCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCt                                                                                            <  4:186114/149‑1 (MQ=255)
                                                      cGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTg                                                                                           >  3:17534/1‑149 (MQ=255)
                                                      cGATGGCCCGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAgg                                                                                                                          <  1:214226/118‑1 (MQ=255)
                                                      cGATGGCCCGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAgg                                                                                                                          >  2:214226/1‑118 (MQ=255)
                                                       gATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTgg                                                                                          <  8:196884/149‑1 (MQ=255)
                                                            ccTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATc                                                                                     >  5:173350/1‑149 (MQ=255)
                                                             cTGCCCATCCGCAAGTTCATCGTGGTACCGGGCAAGCTGGTCAACATCGGCCCAAACTGATTAAAACGCCCACCCGCATCGATTGGGGGTGGGC‑‑‑GGAACAGGCCCAAAAgg                                                                                                                          <  5:54741/111‑1 (MQ=255)
                                                              tGACCATCCGCAAGGTCATTGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCgg                                                                                   >  3:310563/1‑149 (MQ=255)
                                                              tGACCATCCGCAAGGTCATTGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCgg                                                                                   >  3:315556/1‑149 (MQ=255)
                                                                  cATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAATGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGccag                                                                               >  1:60705/1‑147 (MQ=255)
                                                                    tCCTCAAGGTCATCGTGGTACCGTGCAAGCTGTTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGc                                                                             <  6:225820/149‑1 (MQ=255)
                                                                      cGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCgg                                                                           <  2:24274/149‑1 (MQ=255)
                                                                       gCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGt                                                                          <  3:315926/149‑1 (MQ=255)
                                                                           ggTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAggg                                                                                                                         >  7:53668/1‑98 (MQ=255)
                                                                           ggTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAggg                                                                                                                         <  8:53668/98‑1 (MQ=255)
                                                                            gTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGCCTGGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCa                                                                        <  1:265148/149‑1 (MQ=255)
                                                                            gTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGc                                                                     >  5:115426/1‑149 (MQ=255)
                                                                               aTCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCt                                                                    >  5:64459/1‑147 (MQ=255)
                                                                                  gTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAgc                                                                <  6:301688/148‑1 (MQ=255)
                                                                                   tGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAgcgc                                                              <  1:306876/149‑1 (MQ=255)
                                                                                       aCCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGc                                                          >  7:170027/1‑149 (MQ=255)
                                                                                       aCCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATAGGCCTGGCGGTCATGCTCAGCGCCTGc                                                          >  7:108932/1‑149 (MQ=255)
                                                                                        ccGGGCATGGTGGTCATCTTGGTCGCCAACTGATGAAAACGCCCACCCGCAGCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCg                                                         <  8:23648/149‑1 (MQ=255)
                                                                                        ccGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCg                                                         <  3:268704/149‑1 (MQ=255)
                                                                                          gggCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGt                                                       <  7:61150/149‑1 (MQ=255)
                                                                                                gCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAg                                                 >  6:141271/1‑149 (MQ=255)
                                                                                                   ggTCAACATCGTCGCCAACTAATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGc                                                                     >  2:296655/1‑126 (MQ=255)
                                                                                                   ggTCAACATCGTCGCCAACTAATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGc                                                                     <  1:296655/126‑1 (MQ=255)
                                                                                                    gTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGt                                                                                         <  5:311111/105‑1 (MQ=255)
                                                                                                    gTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGt                                                                                         >  6:311111/1‑105 (MQ=255)
                                                                                                    gTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTg                                              <  6:115426/148‑1 (MQ=255)
                                                                                                     tCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgcg                                            >  7:38852/1‑149 (MQ=255)
                                                                                                     tCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGAACAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCa                                                                  >  4:83991/1‑127 (MQ=255)
                                                                                                     tCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGAACAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCa                                                                  <  3:83991/127‑1 (MQ=255)
                                                                                                       aaCATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGcgcg                                          >  1:327541/1‑149 (MQ=255)
                                                                                                          aTCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCa                                       <  6:173350/149‑1 (MQ=255)
                                                                                                          aTCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTAGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCa                                       >  2:22860/1‑149 (MQ=255)
                                                                                                              tcgCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCATCTGCGCGGCACCgg                                   >  6:213751/1‑149 (MQ=255)
                                                                                                                gCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGgc                                  <  8:170027/148‑1 (MQ=255)
                                                                                                                        atgaAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAg                         >  2:303411/1‑149 (MQ=255)
                                                                                                                          gaAATCGCCCACCCGCTTCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGc                        <  8:12581/148‑1 (MQ=255)
                                                                                                                            aaaCGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCt                       >  3:39303/1‑147 (MQ=255)
                                                                                                                            aaaCGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCt                       >  6:303537/1‑147 (MQ=255)
                                                                                                                            aaaCGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGa                     <  6:64459/149‑1 (MQ=255)
                                                                                                                              aCGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGc                   <  8:38852/149‑1 (MQ=255)
                                                                                                                                 cccACCCGCAATCGATGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGt                 >  4:231171/1‑148 (MQ=255)
                                                                                                                                 cccACCCGCAATCGATGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGt                 >  1:21975/1‑148 (MQ=255)
                                                                                                                                  ccACCCGCATCGATTGCGGCTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCACGTAATCGGCCTGTCGGTCATGCTCAGCGCCTGAGGTTTCCAGCTGAGCGGCACAGGCGCCACCGAGCTGAGCCTg                >  6:96867/1‑148 (MQ=255)
                                                                                                                                   cACCCGCAATCGATGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa              <  2:21975/149‑1 (MQ=255)
                                                                                                                                   cACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa              <  4:17534/149‑1 (MQ=255)
                                                                                                                                   cACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa              <  6:122868/149‑1 (MQ=255)
                                                                                                                                   cACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa              <  6:97969/149‑1 (MQ=255)
                                                                                                                                   cACCCGCATCGATTGCGGGTAGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa              <  1:22860/149‑1 (MQ=255)
                                                                                                                                            tccaTTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATgg      <  4:198056/146‑1 (MQ=255)
                                                                                                                                                ttGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGACg   >  8:72619/1‑148 (MQ=255)
                                                                                                                                                ttGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGACGt  >  2:255106/1‑149 (MQ=255)
                                                                                                                                                ttGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGACGt  <  3:97111/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                       
CCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCATCGATTGCGGGTGGGC‑‑‑GGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAATGGACGT  >  NC_002947/5458755‑5459046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: