Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,420,598 Δ1 bp 13.0% intergenic (+81/+26) ctpH → / ← PP_t62 methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,420,5980A.13.0% 157.0 / 19.2 54intergenic (+81/+26)ctpH/PP_t62methyl‑accepting chemotaxis protein CtpH/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base . (3/4);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01

GCGGGGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTG  >  NC_002947/2420452‑2420745
                                                                                                                                                  |                                                                                                                                                    
gCGGGGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGcaca                                                                                                                                                    >  3:117362/1‑149 (MQ=255)
  ggggCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATg                                                                                                                                                  <  2:143027/149‑1 (MQ=255)
         cgcCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCt                                                                                                                                          <  4:109628/149‑1 (MQ=255)
           ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACGTG‑GCGGGCttt                                                                                                                                        <  6:185367/149‑1 (MQ=255)
           ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCttt                                                                                                                                        >  6:65221/1‑149 (MQ=255)
           ccTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCttt                                                                                                                                        <  1:50137/149‑1 (MQ=255)
              gCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt                                                                                                                                      >  5:170528/1‑148 (MQ=255)
               cATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt                                                                                                                                      <  6:278932/147‑1 (MQ=255)
               cATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGa                                                                                                                                    <  5:65221/149‑1 (MQ=255)
                        tCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGAttt                                                                                                                           <  2:309256/149‑1 (MQ=255)
                               aGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGga                                                                                                                    <  7:218870/149‑1 (MQ=255)
                               aGTTGGGCGAAGTCACATCCCTCCTGAGTGCCTGACTCGGTCTGGAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCAGATG‑GCGGGCTTTGTGAGATTGTTTTGTTCGga                                                                                                                    <  4:309661/149‑1 (MQ=255)
                                     gCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAgg                                                                                                                               >  4:141115/1‑132 (MQ=255)
                                     gCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAgg                                                                                                                               <  3:141115/132‑1 (MQ=255)
                                           tCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGAGTc                                                                                                        >  7:184416/1‑149 (MQ=255)
                                            cacaTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTc                                                                                                        >  3:104876/1‑148 (MQ=255)
                                             aCCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCg                                                                                                       >  3:128449/1‑148 (MQ=255)
                                             aCCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCg                                                                                                       >  8:172543/1‑148 (MQ=255)
                                              ccTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCTCACACAACACAAAGCCCGCACATG‑GCGTGCTTTGTGAC‑AGGATTTGGTCGGAGAGACAGGATTCGAAc                                                                                                    <  5:111805/149‑1 (MQ=255)
                                               cTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACa                                                                                                              <  7:82659/139‑1 (MQ=255)
                                               cTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACa                                                                                                              >  8:82659/1‑139 (MQ=255)
                                                  ccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt                                                                                                                                      >  6:227216/1‑112 (MQ=255)
                                                  ccGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGGGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTtgt                                                                                                                                      <  5:227216/112‑1 (MQ=255)
                                                   cGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCACAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGc                                                                                                >  8:175301/1‑149 (MQ=255)
                                                          gTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTtc                                                                                         <  8:71676/149‑1 (MQ=255)
                                                                   tCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg                                                                                                                                     >  7:40430/1‑96 (MQ=38)
                                                                   tCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGC‑CATGTGCGGGCTTtgtg                                                                                                                                     <  8:40430/96‑1 (MQ=38)
                                                                       tCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACg                                                                             >  4:232031/1‑148 (MQ=255)
                                                                          gCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAc                                                                         >  7:13266/1‑149 (MQ=255)
                                                                              gAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACgcgc                                                                     >  3:188868/1‑149 (MQ=255)
                                                                                 ttGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAcg                                                                        >  4:223188/1‑143 (MQ=255)
                                                                                 ttGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGAcg                                                                        <  3:223188/143‑1 (MQ=255)
                                                                                    cAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt                                                                <  8:52288/148‑1 (MQ=255)
                                                                                        tGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTg                                                           <  4:188868/149‑1 (MQ=255)
                                                                                          ccATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTgcg                                                         <  3:257867/149‑1 (MQ=255)
                                                                                          ccATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTgc                                                          <  4:128449/148‑1 (MQ=255)
                                                                                                  gCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGTGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt                                                                <  1:80259/134‑1 (MQ=255)
                                                                                                  gCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCt                                                                >  2:80259/1‑134 (MQ=255)
                                                                                                        gCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGa                                           <  6:170528/149‑1 (MQ=255)
                                                                                                          cgcgCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAAc                                         <  7:172543/149‑1 (MQ=255)
                                                                                                                   aCGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTa                                >  7:194501/1‑149 (MQ=255)
                                                                                                                     gTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACt                              <  7:175301/149‑1 (MQ=255)
                                                                                                                          tttCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATc                         >  3:113685/1‑149 (MQ=255)
                                                                                                                                 cacaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt                  <  3:304498/149‑1 (MQ=255)
                                                                                                                                  acaactcaaTGCCCGCACATG‑GCTGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGTACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTAGCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt                  <  2:34715/148‑1 (MQ=255)
                                                                                                                                  acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttttt                 >  3:149360/1‑149 (MQ=255)
                                                                                                                                  acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttttt                 >  3:154260/1‑149 (MQ=255)
                                                                                                                                  acaacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGtttt                  >  1:34715/1‑148 (MQ=255)
                                                                                                                                    aacacaaAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGc               >  6:293763/1‑149 (MQ=255)
                                                                                                                                      cacaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTc             >  8:245541/1‑149 (MQ=255)
                                                                                                                                       acaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttt                   >  6:272947/1‑142 (MQ=255)
                                                                                                                                       acaaAGCCCGC‑CATGTGCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGttt                   <  5:272947/142‑1 (MQ=255)
                                                                                                                                         aaaGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGc           >  5:2660/1‑148 (MQ=255)
                                                                                                                                         aaaGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTAGc           >  7:11661/1‑148 (MQ=255)
                                                                                                                                          aaGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCaa         <  8:194501/149‑1 (MQ=255)
                                                                                                                                                 cacaTG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCttg  <  4:104876/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCGGGGCAGCGCCTGCATGGTTTGTCAGGGCAGTTGGGCGAAGTCACCTCCCGCCTGAGTGCCTGACTCGGTCTGCAGGAGTTGCAGGTGCCATAGAGGCTGAAGCCGCGCACTGACGTATTTTTCGCACACAACACAAAGCCCGCACATG‑GCGGGCTTTGTGACAAGGATTTGGTCGGAGAGACAGGATTCGAACCTGCGGCCTTCTCGTCCCGAACGAGACGCGCTACCTGGCTGCGCTACACTCCGATGAACAAAATCCTACTGCATCCCGTTTTTGCTCGCAAGCCCTTG  >  NC_002947/2420452‑2420745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑