Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,665,085:1	+T	5.3%	coding (620/834 nt)	PP_4973 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,665,085	1	.	T	5.3%	307.6 / 11.3	75	coding (620/834 nt)	PP_4973	membrane protein
Reads supporting (aligned to +/- strand):  ref base . (37/34);  new base T (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTTTGAGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTGCCCGTCGAACC  >  NC_002947/5664940‑5665232                                                                                                                                                    |                                                                                                                                                    ttCTTTGAGGTAGCGCATGCCCAGCAGCACAACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATCGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑gg                                                                                                                                                   <  2:54421/148‑1 (MQ=255)  tCTTTGAGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑Ggc                                                                                                                                                  <  5:7005/148‑1 (MQ=255)    tttGAGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAATGgcgc                                                                                                                                                >  3:30718/1‑149 (MQ=255)    tttGAGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑Ggcgc                                                                                                                                                >  1:186289/1‑148 (MQ=255)     ttGAGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAATGGCGcc                                                                                                                                               >  4:219230/1‑149 (MQ=255)        aGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGCGCGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCAtt                                                                                                                                            >  1:85142/1‑149 (MQ=255)         ggTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTgg                                                                                                                                          <  6:139495/149‑1 (MQ=255)          gTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACTCTACCGCCAACCCAA‑GGCGCCATTggg                                                                                                                                         <  6:164232/149‑1 (MQ=255)          gTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTggg                                                                                                                                         >  8:178041/1‑149 (MQ=255)            aGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCAttcgtac                                                                                                                                       >  3:182119/1‑144 (MQ=255)               gcATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGc                                                                                                                                    >  5:256459/1‑149 (MQ=255)                cATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGCCCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGc                                                                                                                                    >  3:20449/1‑148 (MQ=255)                cATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAATGGCGCC‑TTGGGTTGGcg                                                                                                                                   <  3:219230/149‑2 (MQ=255)                cATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGc                                                                                                                                    >  8:10996/1‑148 (MQ=255)                cATGCCCAGCAGCACCACCAGGATCACGCTCAACTCACGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGc                                                                                                                                    >  1:196428/1‑148 (MQ=255)                  tGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGCGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGc                                                                                                                                    >  8:144664/1‑146 (MQ=255)                     ccAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAATGGCGCC‑TTGGGTTGGcggtagg                                                                                                                              <  4:30718/149‑7 (MQ=255)                     ccAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGaa                                                                                                                               >  3:181044/1‑148 (MQ=255)                              ccaccaGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAAt                                                                                                                     <  2:180285/149‑1 (MQ=255)                                 ccaGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGgc                                                                                                                  >  4:196164/1‑149 (MQ=255)                                 ccaGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGgc                                                                                                                  <  6:256459/149‑1 (MQ=255)                                 ccaGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGgc                                                                                                                  <  2:85142/149‑1 (MQ=255)                                    ggATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGcgcc                                                                                                               >  4:316754/1‑149 (MQ=255)                                     gATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGcgccc                                                                                                              >  4:267420/1‑149 (MQ=255)                                            cTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCgcgc                                                                                                       >  5:127035/1‑149 (MQ=255)                                            cTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCgcgc                                                                                                       <  5:74110/149‑1 (MQ=255)                                             tCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCCGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCt                                                                                                      >  3:184517/1‑149 (MQ=255)                                             tCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCgcgc                                                                                                       >  5:7202/1‑148 (MQ=255)                                              cAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTa                                                                                                     >  3:7145/1‑149 (MQ=255)                                                  tCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCt                                                                                                 >  7:157620/1‑149 (MQ=255)                                                    ccGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTc                                                                                                <  8:157620/148‑1 (MQ=255)                                                    ccGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACAGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAg                                                                                                                            >  4:125765/1‑120 (MQ=255)                                                    ccGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACAGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAg                                                                                                                            <  3:125765/120‑1 (MQ=255)                                                     cGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGc                                                                                              >  7:116438/1‑149 (MQ=255)                                                        aGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCcaa                                                                                           <  3:316754/149‑1 (MQ=255)                                                            c‑cgccgCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTAc                                                                                                                         >  5:315055/1‑115 (MQ=255)                                                            c‑cgccgCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTAc                                                                                                                         <  6:315055/115‑1 (MQ=255)                                                              cgccgcTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACg                                                                                      <  3:143417/149‑1 (MQ=255)                                                              cgccgcTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACg                                                                                      <  7:178041/149‑1 (MQ=255)                                                                ccgcTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACggg                                                                                    <  8:77455/149‑1 (MQ=255)                                                                      cGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAAtt                                                                                <  5:310372/147‑1 (MQ=255)                                                                      cGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAAtt                                                                                <  8:116438/147‑1 (MQ=255)                                                                         ccACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAg                                                                           <  6:127035/149‑1 (MQ=255)                                                                          cACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAgg                                                                          <  1:177457/149‑1 (MQ=255)                                                                           aCTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGgc                                                                         <  4:181044/149‑1 (MQ=255)                                                                               aCCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCt                                                                      >  5:150532/1‑148 (MQ=255)                                                                                 cccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAg                                                                   >  1:123500/1‑149 (MQ=255)                                                                                 cccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAg                                                                   >  2:70133/1‑149 (MQ=255)                                                                                  ccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGc                                                                              <  5:100798/137‑1 (MQ=255)                                                                                  ccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGc                                                                              >  6:100798/1‑137 (MQ=255)                                                                                  ccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAg                                                                   >  6:254839/1‑148 (MQ=255)                                                                                  ccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGc                                                                  <  5:86018/149‑1 (MQ=255)                                                                                  ccAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGc                                                                  <  3:5632/149‑1 (MQ=255)                                                                                     ggTGCATTGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCTCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAgg                                                               <  2:186289/149‑1 (MQ=255)                                                                                     ggTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAgg                                                               <  4:7145/149‑1 (MQ=255)                                                                                     ggTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAgg                                                               <  7:144664/149‑1 (MQ=255)                                                                                     ggTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAgg                                                               <  3:196164/149‑1 (MQ=255)                                                                                         cATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGc                                                                                              >  8:218515/1‑114 (MQ=255)                                                                                         cATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGc                                                                                              <  7:218515/114‑1 (MQ=255)                                                                                            ggCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGc                                                         >  1:51147/1‑148 (MQ=255)                                                                                             gCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGc                                                         >  3:60919/1‑147 (MQ=255)                                                                                             gCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGCAATTGCCAGGCGCTGAGCAGGGTCAGc                                                         >  2:232467/1‑147 (MQ=255)                                                                                                cacaGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGAc                                                    <  4:165678/149‑1 (MQ=255)                                                                                                        cAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAAt                                            >  5:148802/1‑149 (MQ=255)                                                                                                              aTAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGTGTCAGCCAGACCAGGTAATCCCACg                                      <  2:196428/149‑1 (MQ=255)                                                                                                                  cTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGttt                                  >  2:15236/1‑149 (MQ=255)                                                                                                                   tGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTNNTCCCACGNNTTg                                 >  8:14001/1‑149 (MQ=255)                                                                                                                     aaCAATACGCAGAACCCTACCGCCAACCCCA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGc                                <  6:150532/148‑1 (MQ=255)                                                                                                                               aGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGgcg                      >  8:77579/1‑148 (MQ=255)                                                                                                                               aGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGgcg                      >  6:249887/1‑148 (MQ=255)                                                                                                                               aGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGccg                      >  1:147532/1‑148 (MQ=255)                                                                                                                                        ccGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCt              <  6:7202/147‑1 (MQ=255)                                                                                                                                        ccGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTg             <  4:20449/148‑1 (MQ=255)                                                                                                                                           ccAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTGCCCg         >  5:55830/1‑149 (MQ=255)                                                                                                                                              aCCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTGCCCGTCg      <  4:60919/149‑1 (MQ=255)                                                                                                                                                 cAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTGCCCGTCGAAc   <  3:267420/149‑1 (MQ=255)                                                                                                                                                  aa‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTGCCCGTCGAAcc  <  5:254839/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TTCTTTGAGGTAGCGCATGCCCAGCAGCACCACCAGGATCACGCTCAACTCCCGCAGTGC‑CGCCGCTTCGGCCACTGACCCCAGGTGCATGGCCCACAGCACCAGGGCATAGCTGAACAATACGCAGAACCCTACCGCCAACCCAA‑GGCGCCATTGGGTTCGCCAGAACAGTACGAATGGCGCCCGCCGCGCTACGCTCGCCAACAACGGGAATTGCCAGGCGCTGAGCAGGGTCAGCCAGACCAGGTAATCCCACGGTTTGCCCCACAGGCGCACGGCCTGCCCGTCGAACC  >  NC_002947/5664940‑5665232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑